In vitro culture of Neoechinorhynchus buttnerae (Acanthocephala: Neoechinorhynchidae): Influence of temperature and culture media
Abstract Infection by the acantocephalan Neoechinorhynchus buttnerae is considered one of most important concerns for tambaqui fish (Colossoma macropomum ) production. Treatment strategies have been the focus of several in vivo studies; however, few studies have been undertaken on in vitro protocol...
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Colégio Brasileiro de Parasitologia Veterinaria
2018-11-01
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000400562&lng=en&tlng=en |
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| author | Carinne Moreira de Souza Costa Talissa Beatriz Costa Lima Matheus Gomes da Cruz Daniela Volcan Almeida Maurício Laterça Martins Gabriela Tomas Jerônimo |
| author_facet | Carinne Moreira de Souza Costa Talissa Beatriz Costa Lima Matheus Gomes da Cruz Daniela Volcan Almeida Maurício Laterça Martins Gabriela Tomas Jerônimo |
| author_sort | Carinne Moreira de Souza Costa |
| collection | DOAJ |
| description | Abstract Infection by the acantocephalan Neoechinorhynchus buttnerae is considered one of most important concerns for tambaqui fish (Colossoma macropomum ) production. Treatment strategies have been the focus of several in vivo studies; however, few studies have been undertaken on in vitro protocols for parasite maintenance. The aim of the present study was to develop the best in vitro culture condition for N. buttnerae to ensure its survival and adaptation out of the host to allow for the testing of substances to be used to control the parasite. To achieve this, parasites were collected from naturally infected fish and distributed in 6-well culture plates under the following treatments in triplicate: 0.9% NaCl, sterile tank water, L-15 Leibovitz culture medium, L-15 Leibovitz + agar 2% culture medium, RPMI 1640 culture medium, and RPMI 1640 + agar 2% culture medium. The plates containing the parasites were maintained at 24 °C, 28 °C, and 32 °C. The RPMI 1640 + agar 2% culture medium showed the best survival of 24 days at 24 °C. No body alterations such as swollen parasites, body deformation, dehydration and hardening were observed in the RPMI 1640 + 2% culture medium. |
| first_indexed | 2024-12-12T11:37:23Z |
| format | Article |
| id | doaj.art-eefdc524e726491cb5facfd0dfb3ff81 |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-12-12T11:37:23Z |
| publishDate | 2018-11-01 |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-eefdc524e726491cb5facfd0dfb3ff812022-12-22T00:25:37ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-29612018-11-0127456256910.1590/s1984-296120180079S1984-29612018000400562In vitro culture of Neoechinorhynchus buttnerae (Acanthocephala: Neoechinorhynchidae): Influence of temperature and culture mediaCarinne Moreira de Souza CostaTalissa Beatriz Costa LimaMatheus Gomes da CruzDaniela Volcan AlmeidaMaurício Laterça MartinsGabriela Tomas JerônimoAbstract Infection by the acantocephalan Neoechinorhynchus buttnerae is considered one of most important concerns for tambaqui fish (Colossoma macropomum ) production. Treatment strategies have been the focus of several in vivo studies; however, few studies have been undertaken on in vitro protocols for parasite maintenance. The aim of the present study was to develop the best in vitro culture condition for N. buttnerae to ensure its survival and adaptation out of the host to allow for the testing of substances to be used to control the parasite. To achieve this, parasites were collected from naturally infected fish and distributed in 6-well culture plates under the following treatments in triplicate: 0.9% NaCl, sterile tank water, L-15 Leibovitz culture medium, L-15 Leibovitz + agar 2% culture medium, RPMI 1640 culture medium, and RPMI 1640 + agar 2% culture medium. The plates containing the parasites were maintained at 24 °C, 28 °C, and 32 °C. The RPMI 1640 + agar 2% culture medium showed the best survival of 24 days at 24 °C. No body alterations such as swollen parasites, body deformation, dehydration and hardening were observed in the RPMI 1640 + 2% culture medium.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000400562&lng=en&tlng=enFish farmingtambaquiacantocephalanculturesurvival |
| spellingShingle | Carinne Moreira de Souza Costa Talissa Beatriz Costa Lima Matheus Gomes da Cruz Daniela Volcan Almeida Maurício Laterça Martins Gabriela Tomas Jerônimo In vitro culture of Neoechinorhynchus buttnerae (Acanthocephala: Neoechinorhynchidae): Influence of temperature and culture media Revista Brasileira de Parasitologia Veterinária Fish farming tambaqui acantocephalan culture survival |
| title | In vitro culture of Neoechinorhynchus buttnerae (Acanthocephala: Neoechinorhynchidae): Influence of temperature and culture media |
| title_full | In vitro culture of Neoechinorhynchus buttnerae (Acanthocephala: Neoechinorhynchidae): Influence of temperature and culture media |
| title_fullStr | In vitro culture of Neoechinorhynchus buttnerae (Acanthocephala: Neoechinorhynchidae): Influence of temperature and culture media |
| title_full_unstemmed | In vitro culture of Neoechinorhynchus buttnerae (Acanthocephala: Neoechinorhynchidae): Influence of temperature and culture media |
| title_short | In vitro culture of Neoechinorhynchus buttnerae (Acanthocephala: Neoechinorhynchidae): Influence of temperature and culture media |
| title_sort | in vitro culture of neoechinorhynchus buttnerae acanthocephala neoechinorhynchidae influence of temperature and culture media |
| topic | Fish farming tambaqui acantocephalan culture survival |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000400562&lng=en&tlng=en |
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