Nuances of Responses to Two Sources of Grapevine Leafroll Disease on Pinot Noir Grown in the Field for 17 Years
Grapevine leafroll disease (GLD) is one of the most economically damaging virus diseases in grapevine, with grapevine leafroll-associated virus 1 (GLRaV-1) and grapevine leafroll-associated virus 3 (GLRaV-3) as the main contributors. This study complements a previously published transcriptomic analy...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/6/1333 |
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| author | Jean-Sébastien Reynard Justine Brodard Vivian Zufferey Markus Rienth Paul Gugerli Olivier Schumpp Arnaud G. Blouin |
| author_facet | Jean-Sébastien Reynard Justine Brodard Vivian Zufferey Markus Rienth Paul Gugerli Olivier Schumpp Arnaud G. Blouin |
| author_sort | Jean-Sébastien Reynard |
| collection | DOAJ |
| description | Grapevine leafroll disease (GLD) is one of the most economically damaging virus diseases in grapevine, with grapevine leafroll-associated virus 1 (GLRaV-1) and grapevine leafroll-associated virus 3 (GLRaV-3) as the main contributors. This study complements a previously published transcriptomic analysis and compared the impact of two different forms of GLD to a symptomless control treatment: a mildly symptomatic form infected with GLRaV-1 and a severe form with exceptionally early leafroll symptoms (up to six weeks before veraison) infected with GLRaV-1 and GLRaV-3. Vine physiology and fruit composition in 17-year-old Pinot noir vines were measured and a gradient of vigor, yield, and berry quality (sugar content and berry weight) was observed between treatments. Virome composition, confirmed by individual RT-PCR, was compared with biological indexing. Three divergent viromes were recovered, containing between four to seven viruses and two viroids. They included the first detection of grapevine asteroid mosaic-associated virus in Switzerland. This virus did not cause obvious symptoms on the indicators used in biological indexing. Moreover, the presence of grapevine virus B (GVB) did not cause the expected corky bark symptoms on the indicators, thus underlining the important limitations of the biological indexing. Transmission of GLRaV-3 alone or in combination with GVB by <i>Planococcus comstocki</i> mealybug did not reproduce the strong symptoms observed on the donor plant infected with a severe form of GLD. This result raises questions about the contribution of each virus to the symptomatology of the plant. |
| first_indexed | 2024-03-09T22:13:50Z |
| format | Article |
| id | doaj.art-ef042b75016b46b8a396fc2df53e3bb4 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T22:13:50Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-ef042b75016b46b8a396fc2df53e3bb42023-11-23T19:27:38ZengMDPI AGViruses1999-49152022-06-01146133310.3390/v14061333Nuances of Responses to Two Sources of Grapevine Leafroll Disease on Pinot Noir Grown in the Field for 17 YearsJean-Sébastien Reynard0Justine Brodard1Vivian Zufferey2Markus Rienth3Paul Gugerli4Olivier Schumpp5Arnaud G. Blouin6Groupe Viticulture, Agroscope, 1009 Pully, SwitzerlandVirology-Phytoplasmology Laboratory, Agroscope, 1260 Nyon, SwitzerlandGroupe Viticulture, Agroscope, 1009 Pully, SwitzerlandChangins College for Viticulture and Oenology, University of Sciences and Art Western Switzerland, 1260 Nyon, SwitzerlandVirology-Phytoplasmology Laboratory, Agroscope, 1260 Nyon, SwitzerlandVirology-Phytoplasmology Laboratory, Agroscope, 1260 Nyon, SwitzerlandVirology-Phytoplasmology Laboratory, Agroscope, 1260 Nyon, SwitzerlandGrapevine leafroll disease (GLD) is one of the most economically damaging virus diseases in grapevine, with grapevine leafroll-associated virus 1 (GLRaV-1) and grapevine leafroll-associated virus 3 (GLRaV-3) as the main contributors. This study complements a previously published transcriptomic analysis and compared the impact of two different forms of GLD to a symptomless control treatment: a mildly symptomatic form infected with GLRaV-1 and a severe form with exceptionally early leafroll symptoms (up to six weeks before veraison) infected with GLRaV-1 and GLRaV-3. Vine physiology and fruit composition in 17-year-old Pinot noir vines were measured and a gradient of vigor, yield, and berry quality (sugar content and berry weight) was observed between treatments. Virome composition, confirmed by individual RT-PCR, was compared with biological indexing. Three divergent viromes were recovered, containing between four to seven viruses and two viroids. They included the first detection of grapevine asteroid mosaic-associated virus in Switzerland. This virus did not cause obvious symptoms on the indicators used in biological indexing. Moreover, the presence of grapevine virus B (GVB) did not cause the expected corky bark symptoms on the indicators, thus underlining the important limitations of the biological indexing. Transmission of GLRaV-3 alone or in combination with GVB by <i>Planococcus comstocki</i> mealybug did not reproduce the strong symptoms observed on the donor plant infected with a severe form of GLD. This result raises questions about the contribution of each virus to the symptomatology of the plant.https://www.mdpi.com/1999-4915/14/6/1333viromegrapevine leafroll diseaseHTSbiological indexingtransmission<i>Pseudococcus comstocki</i> |
| spellingShingle | Jean-Sébastien Reynard Justine Brodard Vivian Zufferey Markus Rienth Paul Gugerli Olivier Schumpp Arnaud G. Blouin Nuances of Responses to Two Sources of Grapevine Leafroll Disease on Pinot Noir Grown in the Field for 17 Years Viruses virome grapevine leafroll disease HTS biological indexing transmission <i>Pseudococcus comstocki</i> |
| title | Nuances of Responses to Two Sources of Grapevine Leafroll Disease on Pinot Noir Grown in the Field for 17 Years |
| title_full | Nuances of Responses to Two Sources of Grapevine Leafroll Disease on Pinot Noir Grown in the Field for 17 Years |
| title_fullStr | Nuances of Responses to Two Sources of Grapevine Leafroll Disease on Pinot Noir Grown in the Field for 17 Years |
| title_full_unstemmed | Nuances of Responses to Two Sources of Grapevine Leafroll Disease on Pinot Noir Grown in the Field for 17 Years |
| title_short | Nuances of Responses to Two Sources of Grapevine Leafroll Disease on Pinot Noir Grown in the Field for 17 Years |
| title_sort | nuances of responses to two sources of grapevine leafroll disease on pinot noir grown in the field for 17 years |
| topic | virome grapevine leafroll disease HTS biological indexing transmission <i>Pseudococcus comstocki</i> |
| url | https://www.mdpi.com/1999-4915/14/6/1333 |
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