Study of influence of metal ions in the diagnosis of recombinant hepatitis B surface antigen (HBsAg) using ELISA technique
Objectives: Preliminary diagnosis of hepatitis B is performed by measuring the levels of serological markers using enzyme-linked immunosorbent assay (ELISA). Errors in diagnosis of the disease using serum markers might occur due to the presence of metallic salts in diagnostic samples. The present st...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722006814 |
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| author | Hassan A. Alhazmi Nasser Shubayr Mohammed Albratty Asim Najmi Saeed Alshahrani Hafiz A. Makeen Mohamed Eltaib Elmobark Sivakumar Sivagurunathan Moni Asaad Khalid Waquar Ahsan |
| author_facet | Hassan A. Alhazmi Nasser Shubayr Mohammed Albratty Asim Najmi Saeed Alshahrani Hafiz A. Makeen Mohamed Eltaib Elmobark Sivakumar Sivagurunathan Moni Asaad Khalid Waquar Ahsan |
| author_sort | Hassan A. Alhazmi |
| collection | DOAJ |
| description | Objectives: Preliminary diagnosis of hepatitis B is performed by measuring the levels of serological markers using enzyme-linked immunosorbent assay (ELISA). Errors in diagnosis of the disease using serum markers might occur due to the presence of metallic salts in diagnostic samples. The present study was designed to evaluate the effects of various metal ions on the diagnosis of recombinant hepatitis B surface antigen (HBsAg). Methods: A simple step sandwich ELISA technique was used to evaluate the effects of metal ions on the diagnosis of HBsAg. Further, the zeta potential, electrical conductivity and redox potential of solutions with proteins and metal salts were measured to evaluate the interaction of these metal ions with the protein. Results: The results showed that recombinant HBsAg significantly interacted with the tested metal ions, with lithium ion showing the highest interaction, followed by barium, aluminium, and nickel as the percent decrease in the free HBsAg concentration in presence of these metal ions were found to be 95.5, 94.1, 93.5, and 91.6%, respectively. Zeta potential of the protein in reaction mixture also changed considerably and marked decrease in the concentration of free HBsAg was observed. This decrease in the concentration of free HBsAg resulted into error in the ELISA test which was negative in presence of these metal ions due to strong interaction with the HBsAg protein. Conclusions: The findings of this study clearly demonstrated that the presence of metal ions in pathological samples can result in interaction with the antigen leading to possible diagnostic errors. |
| first_indexed | 2024-04-10T22:53:10Z |
| format | Article |
| id | doaj.art-ef06d05e2b8348b2a8bdbb53c535a29c |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-10T22:53:10Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-ef06d05e2b8348b2a8bdbb53c535a29c2023-01-15T04:21:20ZengElsevierJournal of King Saud University: Science1018-36472023-02-01352102500Study of influence of metal ions in the diagnosis of recombinant hepatitis B surface antigen (HBsAg) using ELISA techniqueHassan A. Alhazmi0Nasser Shubayr1Mohammed Albratty2Asim Najmi3Saeed Alshahrani4Hafiz A. Makeen5Mohamed Eltaib Elmobark6Sivakumar Sivagurunathan Moni7Asaad Khalid8Waquar Ahsan9Department of Pharmaceutical Chemistry and Pharmacognosy, College of Pharmacy, Jazan University, Jazan, Saudi Arabia; Substance Abuse and Toxicology Research Center, Jazan University, Jazan, Saudi ArabiaDiagnostic Radiography Technology Department, College of Applied Medical Sciences, Jazan University, Jazan, Saudi Arabia; Medical Research Center, Jazan University, Jazan, Saudi ArabiaSubstance Abuse and Toxicology Research Center, Jazan University, Jazan, Saudi ArabiaDepartment of Pharmaceutical Chemistry and Pharmacognosy, College of Pharmacy, Jazan University, Jazan, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, Jazan University, Jazan, Saudi ArabiaDepartment of Pharmacy Practice, College of Pharmacy, Jazan University, Jazan, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Jazan University, Jazan, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Jazan University, Jazan, Saudi ArabiaSubstance Abuse and Toxicology Research Center, Jazan University, Jazan, Saudi ArabiaDepartment of Pharmaceutical Chemistry and Pharmacognosy, College of Pharmacy, Jazan University, Jazan, Saudi Arabia; Corresponding author.Objectives: Preliminary diagnosis of hepatitis B is performed by measuring the levels of serological markers using enzyme-linked immunosorbent assay (ELISA). Errors in diagnosis of the disease using serum markers might occur due to the presence of metallic salts in diagnostic samples. The present study was designed to evaluate the effects of various metal ions on the diagnosis of recombinant hepatitis B surface antigen (HBsAg). Methods: A simple step sandwich ELISA technique was used to evaluate the effects of metal ions on the diagnosis of HBsAg. Further, the zeta potential, electrical conductivity and redox potential of solutions with proteins and metal salts were measured to evaluate the interaction of these metal ions with the protein. Results: The results showed that recombinant HBsAg significantly interacted with the tested metal ions, with lithium ion showing the highest interaction, followed by barium, aluminium, and nickel as the percent decrease in the free HBsAg concentration in presence of these metal ions were found to be 95.5, 94.1, 93.5, and 91.6%, respectively. Zeta potential of the protein in reaction mixture also changed considerably and marked decrease in the concentration of free HBsAg was observed. This decrease in the concentration of free HBsAg resulted into error in the ELISA test which was negative in presence of these metal ions due to strong interaction with the HBsAg protein. Conclusions: The findings of this study clearly demonstrated that the presence of metal ions in pathological samples can result in interaction with the antigen leading to possible diagnostic errors.http://www.sciencedirect.com/science/article/pii/S1018364722006814ELISADiagnostic errorsHepatitis B antigenMetal ionsInteraction |
| spellingShingle | Hassan A. Alhazmi Nasser Shubayr Mohammed Albratty Asim Najmi Saeed Alshahrani Hafiz A. Makeen Mohamed Eltaib Elmobark Sivakumar Sivagurunathan Moni Asaad Khalid Waquar Ahsan Study of influence of metal ions in the diagnosis of recombinant hepatitis B surface antigen (HBsAg) using ELISA technique Journal of King Saud University: Science ELISA Diagnostic errors Hepatitis B antigen Metal ions Interaction |
| title | Study of influence of metal ions in the diagnosis of recombinant hepatitis B surface antigen (HBsAg) using ELISA technique |
| title_full | Study of influence of metal ions in the diagnosis of recombinant hepatitis B surface antigen (HBsAg) using ELISA technique |
| title_fullStr | Study of influence of metal ions in the diagnosis of recombinant hepatitis B surface antigen (HBsAg) using ELISA technique |
| title_full_unstemmed | Study of influence of metal ions in the diagnosis of recombinant hepatitis B surface antigen (HBsAg) using ELISA technique |
| title_short | Study of influence of metal ions in the diagnosis of recombinant hepatitis B surface antigen (HBsAg) using ELISA technique |
| title_sort | study of influence of metal ions in the diagnosis of recombinant hepatitis b surface antigen hbsag using elisa technique |
| topic | ELISA Diagnostic errors Hepatitis B antigen Metal ions Interaction |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722006814 |
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