Investigation of Intestinal Microbiota and Fecal Calprotectin in Non-Toxigenic and Toxigenic <i>Clostridioides difficile</i> Colonization and Infection
In this study, we aimed to evaluate the composition of the intestinal microbiota and level of fecal calprotectin in <i>Clostridioides difficile-</i>colonized patients. We included 102 <i>C. difficile</i> non-colonized (group I), 93 <i>C. difficile</i> colonized su...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/6/882 |
| _version_ | 1797417117070393344 |
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| author | Sung-Hee Han Joowon Yi Ji-Hoon Kim Hee-Won Moon |
| author_facet | Sung-Hee Han Joowon Yi Ji-Hoon Kim Hee-Won Moon |
| author_sort | Sung-Hee Han |
| collection | DOAJ |
| description | In this study, we aimed to evaluate the composition of the intestinal microbiota and level of fecal calprotectin in <i>Clostridioides difficile-</i>colonized patients. We included 102 <i>C. difficile</i> non-colonized (group I), 93 <i>C. difficile</i> colonized subjects (group II), and 89 diarrhea patients with <i>C. difficile</i> (group III). Chao1 index for alpha diversity and principal coordinate analysis was performed for beta diversity using QIIME. The mean relative abundance in each group was compared at the phylum and genus levels. Fecal calprotectin was measured using EliA calprotectin (Thermo Fisher Scientific). Group II showed significantly lower levels of <i>Sutterella</i>, <i>Blautia</i>, <i>Ruminococcus</i>, <i>Faecalibacterium</i>, <i>Bilophila</i>, and <i>Ruminococcaceae</i> and higher levels of <i>Enterobacteriaceae</i> compared to group I (<i>p</i> = 0.012, 0.003, 0.002, 0.001, 0.027, 0.022, and 0.036, respectively). Toxigenic <i>C. difficile</i> colonized subjects showed significantly lower levels of <i>Prevotella</i>, <i>Phascolarctobacterium</i>, <i>Succinivibrio</i>, <i>Blautia</i>, and higher levels of <i>Bacteroides</i>. The level of fecal calprotectin in group III was significantly higher than those in group I and group II (<i>p</i> < 0.001 for both). These data could be valuable in understanding <i>C. difficile</i> colonization process and the microbiota and inflammatory markers could be further studied to differentiate colonization from CDI. |
| first_indexed | 2024-03-09T06:14:10Z |
| format | Article |
| id | doaj.art-ef09583180d74cac88980353db444c48 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T06:14:10Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ef09583180d74cac88980353db444c482023-12-03T11:55:16ZengMDPI AGMicroorganisms2076-26072020-06-018688210.3390/microorganisms8060882Investigation of Intestinal Microbiota and Fecal Calprotectin in Non-Toxigenic and Toxigenic <i>Clostridioides difficile</i> Colonization and InfectionSung-Hee Han0Joowon Yi1Ji-Hoon Kim2Hee-Won Moon3BioCore Co. Ltd., Biotechnology, Yongin 64844, KoreaSamkwang Medical Laboratories, Seoul 06742, KoreaAdvanced BioVision Inc., Incheon 21999, KoreaDepartment of Laboratory Medicine, Konkuk University School of Medicine, Seoul 05030, KoreaIn this study, we aimed to evaluate the composition of the intestinal microbiota and level of fecal calprotectin in <i>Clostridioides difficile-</i>colonized patients. We included 102 <i>C. difficile</i> non-colonized (group I), 93 <i>C. difficile</i> colonized subjects (group II), and 89 diarrhea patients with <i>C. difficile</i> (group III). Chao1 index for alpha diversity and principal coordinate analysis was performed for beta diversity using QIIME. The mean relative abundance in each group was compared at the phylum and genus levels. Fecal calprotectin was measured using EliA calprotectin (Thermo Fisher Scientific). Group II showed significantly lower levels of <i>Sutterella</i>, <i>Blautia</i>, <i>Ruminococcus</i>, <i>Faecalibacterium</i>, <i>Bilophila</i>, and <i>Ruminococcaceae</i> and higher levels of <i>Enterobacteriaceae</i> compared to group I (<i>p</i> = 0.012, 0.003, 0.002, 0.001, 0.027, 0.022, and 0.036, respectively). Toxigenic <i>C. difficile</i> colonized subjects showed significantly lower levels of <i>Prevotella</i>, <i>Phascolarctobacterium</i>, <i>Succinivibrio</i>, <i>Blautia</i>, and higher levels of <i>Bacteroides</i>. The level of fecal calprotectin in group III was significantly higher than those in group I and group II (<i>p</i> < 0.001 for both). These data could be valuable in understanding <i>C. difficile</i> colonization process and the microbiota and inflammatory markers could be further studied to differentiate colonization from CDI.https://www.mdpi.com/2076-2607/8/6/882<i>Clostridioides difficile</i> infectioncolonizationmicrobiotacalprotectin |
| spellingShingle | Sung-Hee Han Joowon Yi Ji-Hoon Kim Hee-Won Moon Investigation of Intestinal Microbiota and Fecal Calprotectin in Non-Toxigenic and Toxigenic <i>Clostridioides difficile</i> Colonization and Infection Microorganisms <i>Clostridioides difficile</i> infection colonization microbiota calprotectin |
| title | Investigation of Intestinal Microbiota and Fecal Calprotectin in Non-Toxigenic and Toxigenic <i>Clostridioides difficile</i> Colonization and Infection |
| title_full | Investigation of Intestinal Microbiota and Fecal Calprotectin in Non-Toxigenic and Toxigenic <i>Clostridioides difficile</i> Colonization and Infection |
| title_fullStr | Investigation of Intestinal Microbiota and Fecal Calprotectin in Non-Toxigenic and Toxigenic <i>Clostridioides difficile</i> Colonization and Infection |
| title_full_unstemmed | Investigation of Intestinal Microbiota and Fecal Calprotectin in Non-Toxigenic and Toxigenic <i>Clostridioides difficile</i> Colonization and Infection |
| title_short | Investigation of Intestinal Microbiota and Fecal Calprotectin in Non-Toxigenic and Toxigenic <i>Clostridioides difficile</i> Colonization and Infection |
| title_sort | investigation of intestinal microbiota and fecal calprotectin in non toxigenic and toxigenic i clostridioides difficile i colonization and infection |
| topic | <i>Clostridioides difficile</i> infection colonization microbiota calprotectin |
| url | https://www.mdpi.com/2076-2607/8/6/882 |
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