Transcriptomics and metabolomic profiling identify molecular mechanism for Aspergillus flavus infection in grain
Abstract Aspergillus flavus is a ubiquitous saprophyte and opportunistic fungal pathogen with worldwide distribution, which causes diseases commonly in agricultural commodities and seriously threatens food security and human health. In this study, we try to reveal the mechanisms of A. flavus infecti...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-12-01
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| Series: | Food Frontiers |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fft2.303 |
| _version_ | 1797389178418233344 |
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| author | Chao Ding Tian Tian Qiang Liu Siqi Zhao Tingting Tao Haijing Wu Liping Guo |
| author_facet | Chao Ding Tian Tian Qiang Liu Siqi Zhao Tingting Tao Haijing Wu Liping Guo |
| author_sort | Chao Ding |
| collection | DOAJ |
| description | Abstract Aspergillus flavus is a ubiquitous saprophyte and opportunistic fungal pathogen with worldwide distribution, which causes diseases commonly in agricultural commodities and seriously threatens food security and human health. In this study, we try to reveal the mechanisms of A. flavus infection in grain from the perspective of apparent changes in physicochemical properties, microscopic structure, and ultrastructural changes at transcriptome–metabolomics levels. The results showed that the A. flavus infection included the preinfection (1–3 days) and postinfection period (4–7 days). The downregulated expression of creA promoted the cellulase expression and initiated infection. The metabolite oleic acid promoted infection as a nutrient carbon source and energy source. Then the entry of acetate units into the aflatoxin synthesis pathway was inhibited, whereas aflH, hypD, and hypB were significantly upregulated (p < .05) to stimulate the aflatoxin synthesis to complete the infection on the surface. During the postinfection period, the upregulation of aflS and other toxin‐producing regulatory genes accelerated aflatoxin synthesis and completed the internal infection. At the same time, the upregulation of vitamin B6 and allysine involved in amino acid metabolism, and galactose 1‐phosphate involved in energy metabolism ensured the production of acetyl‐CoA and the energy supply for A. flavus infection. The research findings highlight the underlying mechanisms of A. flavus infection at the transcriptome–metabolomics levels, which is important for developing effective strategies to prevent postharvest contamination. |
| first_indexed | 2024-03-08T22:52:04Z |
| format | Article |
| id | doaj.art-ef0bb09fc6104ed092a5e743ba0e3176 |
| institution | Directory Open Access Journal |
| issn | 2643-8429 |
| language | English |
| last_indexed | 2024-03-08T22:52:04Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Frontiers |
| spelling | doaj.art-ef0bb09fc6104ed092a5e743ba0e31762023-12-16T12:46:30ZengWileyFood Frontiers2643-84292023-12-01441845185810.1002/fft2.303Transcriptomics and metabolomic profiling identify molecular mechanism for Aspergillus flavus infection in grainChao Ding0Tian Tian1Qiang Liu2Siqi Zhao3Tingting Tao4Haijing Wu5Liping Guo6College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing Nanjing University of Finance and Economics Nanjing Jiangsu ChinaCollege of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing Nanjing University of Finance and Economics Nanjing Jiangsu ChinaCollege of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing Nanjing University of Finance and Economics Nanjing Jiangsu ChinaCollege of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing Nanjing University of Finance and Economics Nanjing Jiangsu ChinaSchool of Tea and Food Science Technology Jiangsu Vocational College of Agriculture and Forestry Jurong Jiangsu ChinaNanjing Institute for Food and Drug Control Nanjing Jiangsu ChinaCollege of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing Nanjing University of Finance and Economics Nanjing Jiangsu ChinaAbstract Aspergillus flavus is a ubiquitous saprophyte and opportunistic fungal pathogen with worldwide distribution, which causes diseases commonly in agricultural commodities and seriously threatens food security and human health. In this study, we try to reveal the mechanisms of A. flavus infection in grain from the perspective of apparent changes in physicochemical properties, microscopic structure, and ultrastructural changes at transcriptome–metabolomics levels. The results showed that the A. flavus infection included the preinfection (1–3 days) and postinfection period (4–7 days). The downregulated expression of creA promoted the cellulase expression and initiated infection. The metabolite oleic acid promoted infection as a nutrient carbon source and energy source. Then the entry of acetate units into the aflatoxin synthesis pathway was inhibited, whereas aflH, hypD, and hypB were significantly upregulated (p < .05) to stimulate the aflatoxin synthesis to complete the infection on the surface. During the postinfection period, the upregulation of aflS and other toxin‐producing regulatory genes accelerated aflatoxin synthesis and completed the internal infection. At the same time, the upregulation of vitamin B6 and allysine involved in amino acid metabolism, and galactose 1‐phosphate involved in energy metabolism ensured the production of acetyl‐CoA and the energy supply for A. flavus infection. The research findings highlight the underlying mechanisms of A. flavus infection at the transcriptome–metabolomics levels, which is important for developing effective strategies to prevent postharvest contamination.https://doi.org/10.1002/fft2.303Aspergillus flavusgraininfectionmetabolomicstranscriptomics |
| spellingShingle | Chao Ding Tian Tian Qiang Liu Siqi Zhao Tingting Tao Haijing Wu Liping Guo Transcriptomics and metabolomic profiling identify molecular mechanism for Aspergillus flavus infection in grain Food Frontiers Aspergillus flavus grain infection metabolomics transcriptomics |
| title | Transcriptomics and metabolomic profiling identify molecular mechanism for Aspergillus flavus infection in grain |
| title_full | Transcriptomics and metabolomic profiling identify molecular mechanism for Aspergillus flavus infection in grain |
| title_fullStr | Transcriptomics and metabolomic profiling identify molecular mechanism for Aspergillus flavus infection in grain |
| title_full_unstemmed | Transcriptomics and metabolomic profiling identify molecular mechanism for Aspergillus flavus infection in grain |
| title_short | Transcriptomics and metabolomic profiling identify molecular mechanism for Aspergillus flavus infection in grain |
| title_sort | transcriptomics and metabolomic profiling identify molecular mechanism for aspergillus flavus infection in grain |
| topic | Aspergillus flavus grain infection metabolomics transcriptomics |
| url | https://doi.org/10.1002/fft2.303 |
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