Presence of Multidrug Resistance (MDR) Escherichia coli in the Citarum River located in Greater Bandung Area, Indonesia
Background: One of the main problems of the Citarum River is the contamination of E. coli due to livestock activities, washing toilets, and industry. In addition, irrational use of antibiotics in the community and livestock can increase E. coli resistant strains to antibiotics. This study aimed to i...
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| Format: | Article |
| Language: | English |
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Universitas Padjadjaran
2023-09-01
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| Series: | Althea Medical Journal |
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| Online Access: | https://journal.fk.unpad.ac.id/index.php/amj/article/view/2765 |
| _version_ | 1797372756095926272 |
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| author | Imam Megantara Azmi Raffi Muhtado Gita Widya Pradini Hanna Goenawan Nova Sylviana |
| author_facet | Imam Megantara Azmi Raffi Muhtado Gita Widya Pradini Hanna Goenawan Nova Sylviana |
| author_sort | Imam Megantara |
| collection | DOAJ |
| description | Background: One of the main problems of the Citarum River is the contamination of E. coli due to livestock activities, washing toilets, and industry. In addition, irrational use of antibiotics in the community and livestock can increase E. coli resistant strains to antibiotics. This study aimed to identify the presence of multidrug resistance (MDR) and extended-spectrum β-lactamase (ESBL) E. coli strains in Citarum river clusters, namely industrial, livestock, and residential clusters.
Methods: This was a descriptive study. A sample of 100 mL surface water from each Citarum cluster. Culture, antibiotic sensitivity test, and PCR to identify blaCTX-M-15 gene carriers of ESBL E. coli were carried out in the sample.
Results: There were 37 isolates of E. coli, with 24% of these isolates showing MDR properties, which can be found in industrial, livestock, and residential clusters at 13%, 8%, and 3% respectively. The most E. coli-resistant antibiotics found in these samples were ampicillin (45%), followed by tetracycline (37%), and azithromycin (29%). The PCR examination did not find the blaCTX-M-15 gene carrying ESBL properties in all three Citarum river clusters.
Conclusion: The presence of E. coli isolates in each Citarum river cluster suggests the occurrence of river pollution due to animal, human or industrial waste. Therefore, it is necessary to make better government regulations regarding sanitation and education for the surrounding community regarding the importance of keeping the river clean. |
| first_indexed | 2024-03-08T18:40:25Z |
| format | Article |
| id | doaj.art-ef0c4f272593489b91466cdb3ef92605 |
| institution | Directory Open Access Journal |
| issn | 2337-4330 |
| language | English |
| last_indexed | 2024-03-08T18:40:25Z |
| publishDate | 2023-09-01 |
| publisher | Universitas Padjadjaran |
| record_format | Article |
| series | Althea Medical Journal |
| spelling | doaj.art-ef0c4f272593489b91466cdb3ef926052023-12-29T08:54:35ZengUniversitas PadjadjaranAlthea Medical Journal2337-43302023-09-0110310.15850/amj.v10n3.27651620Presence of Multidrug Resistance (MDR) Escherichia coli in the Citarum River located in Greater Bandung Area, IndonesiaImam Megantara0Azmi Raffi Muhtado1Gita Widya Pradini2Hanna Goenawan3Nova Sylviana4Department of Biomedical Sciences, Faculty of Medicine Universitas PadjadjaranFaculty of Medicine Universitas PadjadjaranDepartment of Biomedical Sciences, Faculty of Medicine Universitas PadjadjaranDepartment of Biomedical Sciences, Faculty of Medicine Universitas PadjadjaranDepartment of Biomedical Sciences, Faculty of Medicine Universitas PadjadjaranBackground: One of the main problems of the Citarum River is the contamination of E. coli due to livestock activities, washing toilets, and industry. In addition, irrational use of antibiotics in the community and livestock can increase E. coli resistant strains to antibiotics. This study aimed to identify the presence of multidrug resistance (MDR) and extended-spectrum β-lactamase (ESBL) E. coli strains in Citarum river clusters, namely industrial, livestock, and residential clusters. Methods: This was a descriptive study. A sample of 100 mL surface water from each Citarum cluster. Culture, antibiotic sensitivity test, and PCR to identify blaCTX-M-15 gene carriers of ESBL E. coli were carried out in the sample. Results: There were 37 isolates of E. coli, with 24% of these isolates showing MDR properties, which can be found in industrial, livestock, and residential clusters at 13%, 8%, and 3% respectively. The most E. coli-resistant antibiotics found in these samples were ampicillin (45%), followed by tetracycline (37%), and azithromycin (29%). The PCR examination did not find the blaCTX-M-15 gene carrying ESBL properties in all three Citarum river clusters. Conclusion: The presence of E. coli isolates in each Citarum river cluster suggests the occurrence of river pollution due to animal, human or industrial waste. Therefore, it is necessary to make better government regulations regarding sanitation and education for the surrounding community regarding the importance of keeping the river clean.https://journal.fk.unpad.ac.id/index.php/amj/article/view/2765escherichia colicitarumextended-spectrum β-lactamasemultidrug-resistance |
| spellingShingle | Imam Megantara Azmi Raffi Muhtado Gita Widya Pradini Hanna Goenawan Nova Sylviana Presence of Multidrug Resistance (MDR) Escherichia coli in the Citarum River located in Greater Bandung Area, Indonesia Althea Medical Journal escherichia coli citarum extended-spectrum β-lactamase multidrug-resistance |
| title | Presence of Multidrug Resistance (MDR) Escherichia coli in the Citarum River located in Greater Bandung Area, Indonesia |
| title_full | Presence of Multidrug Resistance (MDR) Escherichia coli in the Citarum River located in Greater Bandung Area, Indonesia |
| title_fullStr | Presence of Multidrug Resistance (MDR) Escherichia coli in the Citarum River located in Greater Bandung Area, Indonesia |
| title_full_unstemmed | Presence of Multidrug Resistance (MDR) Escherichia coli in the Citarum River located in Greater Bandung Area, Indonesia |
| title_short | Presence of Multidrug Resistance (MDR) Escherichia coli in the Citarum River located in Greater Bandung Area, Indonesia |
| title_sort | presence of multidrug resistance mdr escherichia coli in the citarum river located in greater bandung area indonesia |
| topic | escherichia coli citarum extended-spectrum β-lactamase multidrug-resistance |
| url | https://journal.fk.unpad.ac.id/index.php/amj/article/view/2765 |
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