Comparison of Multiplex Nested RT-PCR with Virus Isolation for Detection of Influenza Viruses A and B
Background: Influenza A viruses bearing all known HA and NA subtypes have been isolated from avian hosts: but only the A/H1N1,A/H2N2 and A/H3N2 influenza subtypes have been associated with widespread epidemics in humans un­til now .While influenza B detected only in humans. Subtype H5 in...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2007-09-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/3808.pdf&manuscript_id=3808 |
| _version_ | 1818741128705343488 |
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| author | T Mokhtari-Azad F Rezaie-Khollari AR Nadji V Salimi Z Noroozbabaie M Naseri AR Mirzaie Z Saadatmand MM Gouya AR Esteghamati R Hamkar |
| author_facet | T Mokhtari-Azad F Rezaie-Khollari AR Nadji V Salimi Z Noroozbabaie M Naseri AR Mirzaie Z Saadatmand MM Gouya AR Esteghamati R Hamkar |
| author_sort | T Mokhtari-Azad |
| collection | DOAJ |
| description | Background: Influenza A viruses bearing all known HA and NA subtypes have been isolated from avian hosts: but only the A/H1N1,A/H2N2 and A/H3N2 influenza subtypes have been associated with widespread epidemics in humans un­til now .While influenza B detected only in humans. Subtype H5 infection in humans occurred for the first time dur­ing 1997-1998 in Hong Kong, then China in 2003, Vietnam and Thailand in 2004 and continued to 2005-2006 in Tur­key and Egypt. In this study a Multiplex Nested RT-PCR was developed for simultaneous detection of influenza virus A and B in clinical samples. Methods: Throat swab and throat wash was collected between October 2005 and September 2006 from 689 patients with a suspected influenza virus infection. Samples were transported to the National Influenza Center (Tehran Univer­sity of Medical Science, School of Public Health). Each sample is divided into two parts in order to virus isolation and nested RT-PCR. Results: The RT-PCR detected in 75 cases of influenza A and B, compared to 26 cases by virus isolation and 74 cases are positive by RT-PCR. In total, 50 out of 74 RT-PCR positive specimens were negative by virus isolation. The RT-PCR provides a sensitive and specific method for detecting and typing influenza viruses A and B. Conclusion: The RT-PCR provides a sensitive and specific method for detecting and typing influenza viruses A and B. Multi­plex RT-PCR can also be used to accurately detect more than one viral template in the same reaction mixture, allow­ing viral confections to be identified with the same respiratory specimen. |
| first_indexed | 2024-12-18T01:51:42Z |
| format | Article |
| id | doaj.art-ef0c591c2d7549b1938568e50c6b73af |
| institution | Directory Open Access Journal |
| issn | 2251-6085 |
| language | English |
| last_indexed | 2024-12-18T01:51:42Z |
| publishDate | 2007-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-ef0c591c2d7549b1938568e50c6b73af2022-12-21T21:25:01ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852007-09-0136317Comparison of Multiplex Nested RT-PCR with Virus Isolation for Detection of Influenza Viruses A and BT Mokhtari-AzadF Rezaie-KhollariAR NadjiV SalimiZ NoroozbabaieM NaseriAR MirzaieZ SaadatmandMM GouyaAR EsteghamatiR HamkarBackground: Influenza A viruses bearing all known HA and NA subtypes have been isolated from avian hosts: but only the A/H1N1,A/H2N2 and A/H3N2 influenza subtypes have been associated with widespread epidemics in humans un­til now .While influenza B detected only in humans. Subtype H5 infection in humans occurred for the first time dur­ing 1997-1998 in Hong Kong, then China in 2003, Vietnam and Thailand in 2004 and continued to 2005-2006 in Tur­key and Egypt. In this study a Multiplex Nested RT-PCR was developed for simultaneous detection of influenza virus A and B in clinical samples. Methods: Throat swab and throat wash was collected between October 2005 and September 2006 from 689 patients with a suspected influenza virus infection. Samples were transported to the National Influenza Center (Tehran Univer­sity of Medical Science, School of Public Health). Each sample is divided into two parts in order to virus isolation and nested RT-PCR. Results: The RT-PCR detected in 75 cases of influenza A and B, compared to 26 cases by virus isolation and 74 cases are positive by RT-PCR. In total, 50 out of 74 RT-PCR positive specimens were negative by virus isolation. The RT-PCR provides a sensitive and specific method for detecting and typing influenza viruses A and B. Conclusion: The RT-PCR provides a sensitive and specific method for detecting and typing influenza viruses A and B. Multi­plex RT-PCR can also be used to accurately detect more than one viral template in the same reaction mixture, allow­ing viral confections to be identified with the same respiratory specimen.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/3808.pdf&manuscript_id=3808Influenza viruses |
| spellingShingle | T Mokhtari-Azad F Rezaie-Khollari AR Nadji V Salimi Z Noroozbabaie M Naseri AR Mirzaie Z Saadatmand MM Gouya AR Esteghamati R Hamkar Comparison of Multiplex Nested RT-PCR with Virus Isolation for Detection of Influenza Viruses A and B Iranian Journal of Public Health Influenza viruses |
| title | Comparison of Multiplex Nested RT-PCR with Virus Isolation for Detection of Influenza Viruses A and B |
| title_full | Comparison of Multiplex Nested RT-PCR with Virus Isolation for Detection of Influenza Viruses A and B |
| title_fullStr | Comparison of Multiplex Nested RT-PCR with Virus Isolation for Detection of Influenza Viruses A and B |
| title_full_unstemmed | Comparison of Multiplex Nested RT-PCR with Virus Isolation for Detection of Influenza Viruses A and B |
| title_short | Comparison of Multiplex Nested RT-PCR with Virus Isolation for Detection of Influenza Viruses A and B |
| title_sort | comparison of multiplex nested rt pcr with vi rus isolation for detection of influenza viruses a and b |
| topic | Influenza viruses |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/3808.pdf&manuscript_id=3808 |
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