Skp2 expression unfavorably impacts survival in resectable esophageal squamous cell carcinoma
<p>Abstract</p> <p>Background</p> <p>The correlation of S-phase kinase–associated protein 2 (Skp2) with metastasis and prognosis in esophageal squamous cell carcinoma (ESCC) is controversial. The purpose of this study was to explore whether there was a correlation betwe...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-05-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://www.translational-medicine.com/content/10/1/73 |
| _version_ | 1818472818475532288 |
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| author | Liang Yi Hou Xue Cui Qian Kang Tie-Bang Fu Jian-Hua Zhang Lan-Jun Luo Rong-Zhen He Jie-Hua Zeng Yi-Xin Yang Hao-Xian |
| author_facet | Liang Yi Hou Xue Cui Qian Kang Tie-Bang Fu Jian-Hua Zhang Lan-Jun Luo Rong-Zhen He Jie-Hua Zeng Yi-Xin Yang Hao-Xian |
| author_sort | Liang Yi |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The correlation of S-phase kinase–associated protein 2 (Skp2) with metastasis and prognosis in esophageal squamous cell carcinoma (ESCC) is controversial. The purpose of this study was to explore whether there was a correlation between the expression of Skp2 evaluated by immunohistochemistry and the clinical outcome of patients with operable ESCC, and to further determine the possible mechanism of the impact of Skp2 on survival.</p> <p>Methods</p> <p>Tissue microarrays that included 157 surgically resected ESCC specimens was successfully generated for immunohistochemical evaluation. The clinical/prognostic significance of Skp2 expression was analyzed. Kaplan-Meier analysis was used to compare the postoperative survival between groups. The prognostic impact of clinicopathologic variables and Skp2 expression was evaluated using a Cox proportional hazards model. A cell proliferation assay and a colony formation assay were performed in ESCC cell lines to determine the function of Skp2 on the progression of ESCC <it>in vitro</it>.</p> <p>Results</p> <p>Skp2 expression correlated closely with the T category (<it>p</it> = 0.035) and the pathological tumor-node-metastasis (TNM) stage (<it>p</it> = 0.027). High expression of Skp2 was associated with poor overall survival in resectable ESCC (<it>p</it> = 0.01). The multivariate Cox regression analysis demonstrated that pathological T category, pathological N category, cell differentiation, and negative Skp2 expression were independent factors for better overall survival. <it>In vitro</it> assays of ESCC cell lines demonstrated that Skp2 promoted the proliferative and colony-forming capacity of ESCCs.</p> <p>Conclusions</p> <p>Negative Skp2 expression in primary resected ESCC is an independent factor for better survival. Skp2 may play a pro-proliferative role in ESCC cells.</p> |
| first_indexed | 2024-04-14T04:12:25Z |
| format | Article |
| id | doaj.art-ef0ccd537d314b8d92d2cb7c5dde2a62 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-04-14T04:12:25Z |
| publishDate | 2012-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-ef0ccd537d314b8d92d2cb7c5dde2a622022-12-22T02:13:04ZengBMCJournal of Translational Medicine1479-58762012-05-011017310.1186/1479-5876-10-73Skp2 expression unfavorably impacts survival in resectable esophageal squamous cell carcinomaLiang YiHou XueCui QianKang Tie-BangFu Jian-HuaZhang Lan-JunLuo Rong-ZhenHe Jie-HuaZeng Yi-XinYang Hao-Xian<p>Abstract</p> <p>Background</p> <p>The correlation of S-phase kinase–associated protein 2 (Skp2) with metastasis and prognosis in esophageal squamous cell carcinoma (ESCC) is controversial. The purpose of this study was to explore whether there was a correlation between the expression of Skp2 evaluated by immunohistochemistry and the clinical outcome of patients with operable ESCC, and to further determine the possible mechanism of the impact of Skp2 on survival.</p> <p>Methods</p> <p>Tissue microarrays that included 157 surgically resected ESCC specimens was successfully generated for immunohistochemical evaluation. The clinical/prognostic significance of Skp2 expression was analyzed. Kaplan-Meier analysis was used to compare the postoperative survival between groups. The prognostic impact of clinicopathologic variables and Skp2 expression was evaluated using a Cox proportional hazards model. A cell proliferation assay and a colony formation assay were performed in ESCC cell lines to determine the function of Skp2 on the progression of ESCC <it>in vitro</it>.</p> <p>Results</p> <p>Skp2 expression correlated closely with the T category (<it>p</it> = 0.035) and the pathological tumor-node-metastasis (TNM) stage (<it>p</it> = 0.027). High expression of Skp2 was associated with poor overall survival in resectable ESCC (<it>p</it> = 0.01). The multivariate Cox regression analysis demonstrated that pathological T category, pathological N category, cell differentiation, and negative Skp2 expression were independent factors for better overall survival. <it>In vitro</it> assays of ESCC cell lines demonstrated that Skp2 promoted the proliferative and colony-forming capacity of ESCCs.</p> <p>Conclusions</p> <p>Negative Skp2 expression in primary resected ESCC is an independent factor for better survival. Skp2 may play a pro-proliferative role in ESCC cells.</p>http://www.translational-medicine.com/content/10/1/73Esophageal surgerySkp2StatisticsSurvival analysis |
| spellingShingle | Liang Yi Hou Xue Cui Qian Kang Tie-Bang Fu Jian-Hua Zhang Lan-Jun Luo Rong-Zhen He Jie-Hua Zeng Yi-Xin Yang Hao-Xian Skp2 expression unfavorably impacts survival in resectable esophageal squamous cell carcinoma Journal of Translational Medicine Esophageal surgery Skp2 Statistics Survival analysis |
| title | Skp2 expression unfavorably impacts survival in resectable esophageal squamous cell carcinoma |
| title_full | Skp2 expression unfavorably impacts survival in resectable esophageal squamous cell carcinoma |
| title_fullStr | Skp2 expression unfavorably impacts survival in resectable esophageal squamous cell carcinoma |
| title_full_unstemmed | Skp2 expression unfavorably impacts survival in resectable esophageal squamous cell carcinoma |
| title_short | Skp2 expression unfavorably impacts survival in resectable esophageal squamous cell carcinoma |
| title_sort | skp2 expression unfavorably impacts survival in resectable esophageal squamous cell carcinoma |
| topic | Esophageal surgery Skp2 Statistics Survival analysis |
| url | http://www.translational-medicine.com/content/10/1/73 |
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