Gross Chromosomal Rearrangements in <i>Kluyveromyces marxianus</i> Revealed by Illumina and Oxford Nanopore Sequencing
<i>Kluyveromyces marxianus</i> (<i>K. marxianus</i>) is an increasingly popular industrially relevant yeast. It is known to possess a highly efficient non-homologous end joining (NHEJ) pathway that promotes random integration of non-homologous DNA fragments into its genome. T...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-09-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/19/7112 |
| Summary: | <i>Kluyveromyces marxianus</i> (<i>K. marxianus</i>) is an increasingly popular industrially relevant yeast. It is known to possess a highly efficient non-homologous end joining (NHEJ) pathway that promotes random integration of non-homologous DNA fragments into its genome. The nature of the integration events was traditionally analyzed by Southern blot hybridization. However, the precise DNA sequence at the insertion sites were not fully explored. We transformed a PCR product of the <i>Saccharomyces cerevisiae URA3</i> gene (<i>ScURA3</i>) into an uracil auxotroph <i>K. marxianus</i> otherwise wildtype strain and picked 24 stable Ura+ transformants for sequencing analysis. We took advantage of rapid advances in DNA sequencing technologies and developed a method using a combination of Illumina MiSeq and Oxford Nanopore sequencing. This approach enables us to uncover the gross chromosomal rearrangements (GCRs) that are associated with the <i>ScURA3</i> random integration. Moreover, it will shine a light on understanding DNA repair mechanisms in eukaryotes, which could potentially provide insights for cancer research. |
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| ISSN: | 1661-6596 1422-0067 |