Determination of some antibiotic residues (tetracycline, oxytetracycline and penicillin-G) in beef sold for public consumption at Dukem and Bishoftu (Debre Zeyit) towns, central Ethiopia by LC/MS/MS
AbstractAntibiotic use is an integral part of livestock farms, but their overuse or misuse may result in the occurrence of unsafe level of residues that cause deleterious health effects. A cross-sectional study was performed from December 10/2020 and January 15/2021 to detect and estimate the level...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Cogent Food & Agriculture |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/23311932.2023.2242633 |
| _version_ | 1797435559871774720 |
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| author | Gudeta Uma Ayenew Ashenef |
| author_facet | Gudeta Uma Ayenew Ashenef |
| author_sort | Gudeta Uma |
| collection | DOAJ |
| description | AbstractAntibiotic use is an integral part of livestock farms, but their overuse or misuse may result in the occurrence of unsafe level of residues that cause deleterious health effects. A cross-sectional study was performed from December 10/2020 and January 15/2021 to detect and estimate the level of tetracycline, oxytetracycline and penicillin G residues in beef samples intended for public consumption at Dukem and Bishoftu towns, central Ethiopia. Muscle samples were randomly collected from butcheries. Residue levels were determined using liquid chromatography coupled with triple quadrupole mass spectrometer (LC- ESI-QqQ). From 100 beef samples analysed, 10 % contained detectable level of residues of oxytetracycline. It is distributed as 12 % (6/50) at Dukem and 8 % (4/50) at Bishoftu towns. Tetracycline as residue was detected only in one sample that was collected from Dukem, at 9.35 µg/kg simultaneously with oxytetracycline. However, penicillin G was not detected in any of the samples. The levels of oxytetracycline residues (in µg/kg) range from 18.9 to 26.1 at Dukem and from 7.63 to 19.73 at Bishoftu. The mean determined residue levels of oxytetracycline were 22.32 ± 2.38 µg/kg and 15.99 ± 13.2 µg/kg at the two towns, respectively. The levels were much lower than the Maximum Residue Limit (MRL) established for Tetracyclines (TCs) either by EU (100 μg/kg) or Codex (200 μg/kg) guidelines. The determined residue levels are minimum and thus minimizing health risks associated with their consumption, although their detection in few samples and their repeated ingestion might be still associated with a rising concern of antibiotic resistance. Thus, the beef in the study area was acceptable for public consumption with respect to the analyzed three antibiotics. |
| first_indexed | 2024-03-09T10:49:28Z |
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| issn | 2331-1932 |
| language | English |
| last_indexed | 2024-03-09T10:49:28Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cogent Food & Agriculture |
| spelling | doaj.art-ef0fc71a9308472a89c654282d5199262023-12-01T08:31:55ZengTaylor & Francis GroupCogent Food & Agriculture2331-19322023-12-019110.1080/23311932.2023.2242633Determination of some antibiotic residues (tetracycline, oxytetracycline and penicillin-G) in beef sold for public consumption at Dukem and Bishoftu (Debre Zeyit) towns, central Ethiopia by LC/MS/MSGudeta Uma0Ayenew Ashenef1Animal Products, Veterinary Drug and Feed Quality Assessment Centre, Agricultural Authority of Ethiopia, Addis Ababa, EthiopiaDepartment of Pharmaceutical Chemistry and Pharmacognosy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, EthiopiaAbstractAntibiotic use is an integral part of livestock farms, but their overuse or misuse may result in the occurrence of unsafe level of residues that cause deleterious health effects. A cross-sectional study was performed from December 10/2020 and January 15/2021 to detect and estimate the level of tetracycline, oxytetracycline and penicillin G residues in beef samples intended for public consumption at Dukem and Bishoftu towns, central Ethiopia. Muscle samples were randomly collected from butcheries. Residue levels were determined using liquid chromatography coupled with triple quadrupole mass spectrometer (LC- ESI-QqQ). From 100 beef samples analysed, 10 % contained detectable level of residues of oxytetracycline. It is distributed as 12 % (6/50) at Dukem and 8 % (4/50) at Bishoftu towns. Tetracycline as residue was detected only in one sample that was collected from Dukem, at 9.35 µg/kg simultaneously with oxytetracycline. However, penicillin G was not detected in any of the samples. The levels of oxytetracycline residues (in µg/kg) range from 18.9 to 26.1 at Dukem and from 7.63 to 19.73 at Bishoftu. The mean determined residue levels of oxytetracycline were 22.32 ± 2.38 µg/kg and 15.99 ± 13.2 µg/kg at the two towns, respectively. The levels were much lower than the Maximum Residue Limit (MRL) established for Tetracyclines (TCs) either by EU (100 μg/kg) or Codex (200 μg/kg) guidelines. The determined residue levels are minimum and thus minimizing health risks associated with their consumption, although their detection in few samples and their repeated ingestion might be still associated with a rising concern of antibiotic resistance. Thus, the beef in the study area was acceptable for public consumption with respect to the analyzed three antibiotics.https://www.tandfonline.com/doi/10.1080/23311932.2023.2242633antibiotic residuepenicillin GtetracyclineoxytetracyclinebeefLC- ESI-MS/MS |
| spellingShingle | Gudeta Uma Ayenew Ashenef Determination of some antibiotic residues (tetracycline, oxytetracycline and penicillin-G) in beef sold for public consumption at Dukem and Bishoftu (Debre Zeyit) towns, central Ethiopia by LC/MS/MS Cogent Food & Agriculture antibiotic residue penicillin G tetracycline oxytetracycline beef LC- ESI-MS/MS |
| title | Determination of some antibiotic residues (tetracycline, oxytetracycline and penicillin-G) in beef sold for public consumption at Dukem and Bishoftu (Debre Zeyit) towns, central Ethiopia by LC/MS/MS |
| title_full | Determination of some antibiotic residues (tetracycline, oxytetracycline and penicillin-G) in beef sold for public consumption at Dukem and Bishoftu (Debre Zeyit) towns, central Ethiopia by LC/MS/MS |
| title_fullStr | Determination of some antibiotic residues (tetracycline, oxytetracycline and penicillin-G) in beef sold for public consumption at Dukem and Bishoftu (Debre Zeyit) towns, central Ethiopia by LC/MS/MS |
| title_full_unstemmed | Determination of some antibiotic residues (tetracycline, oxytetracycline and penicillin-G) in beef sold for public consumption at Dukem and Bishoftu (Debre Zeyit) towns, central Ethiopia by LC/MS/MS |
| title_short | Determination of some antibiotic residues (tetracycline, oxytetracycline and penicillin-G) in beef sold for public consumption at Dukem and Bishoftu (Debre Zeyit) towns, central Ethiopia by LC/MS/MS |
| title_sort | determination of some antibiotic residues tetracycline oxytetracycline and penicillin g in beef sold for public consumption at dukem and bishoftu debre zeyit towns central ethiopia by lc ms ms |
| topic | antibiotic residue penicillin G tetracycline oxytetracycline beef LC- ESI-MS/MS |
| url | https://www.tandfonline.com/doi/10.1080/23311932.2023.2242633 |
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