Testicular Characteristics and the Block to Spermatogenesis in Mature Hinny
Most hinnies (female donkey×male horse) and mules (female horse×male donkey) are sterile with few reports of equine fertile hybrids. The main cause of this sterility is thought to be a meiotic block to spermatogenesis and oogenesis. This study compared the developmental features of the testes and a...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2016-06-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-29-6-793.pdf |
| _version_ | 1818196316754280448 |
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| author | Hongmei Han Aihong Wang Liming Liu Gaoping Zhao Jie Su Biao Wang Yunxia Li Jindun Zhang Baojiang Wu Wei Sun Shuxiang Hu Shuyu Li Lixia Zhao Xihe Li |
| author_facet | Hongmei Han Aihong Wang Liming Liu Gaoping Zhao Jie Su Biao Wang Yunxia Li Jindun Zhang Baojiang Wu Wei Sun Shuxiang Hu Shuyu Li Lixia Zhao Xihe Li |
| author_sort | Hongmei Han |
| collection | DOAJ |
| description | Most hinnies (female donkey×male horse) and mules (female horse×male donkey) are sterile with few reports of equine fertile hybrids. The main cause of this sterility is thought to be a meiotic block to spermatogenesis and oogenesis. This study compared the developmental features of the testes and a histological analyses of spermatogenesis in a male hinny with those of a normal, fertile stallion and Jack donkey. Hinny testes showed a thicker tunica albuginea, fewer blood vessels and more connective tissue in the testis parenchyma than those of the stallion and Jack donkey. Although the mean number of seminiferous tubules was significantly higher in stallion and hinny than Jack donkey (p<0.01), the mean proportion of seminiferous tubules was lower in the hinny (p<0.01) which resulted in a smaller diameter of seminiferous tubules. The mean number of spermatogonia and spermatocytes per unit area were significantly lower in hinny testis (p<0.01) and no spermatids or mature spermatozoa cells were found during immunofluorescent analyses. These results indicated that defects in seminiferous tubule development and structure occur in the testis of hinnies. Furthermore, most spermatogonia and spermatocytes cease development in synapsis during mid-meiosis of spermatocytes, which results in a block to spermatogenesis that prevents the formation of spermatids and matured spermatozoa during meiosis in male hinnies. |
| first_indexed | 2024-12-12T01:32:09Z |
| format | Article |
| id | doaj.art-ef0fec16ef3f4495a5203917323b0f97 |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-12T01:32:09Z |
| publishDate | 2016-06-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-ef0fec16ef3f4495a5203917323b0f972022-12-22T00:42:57ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172016-06-0129679380010.5713/ajas.15.067023447Testicular Characteristics and the Block to Spermatogenesis in Mature HinnyHongmei Han0Aihong Wang1Liming Liu2Gaoping Zhao3Jie Su4Biao Wang5Yunxia Li6Jindun Zhang7Baojiang Wu8Wei Sun9Shuxiang Hu10Shuyu Li11Lixia Zhao12Xihe Li13 Research Center for Animal Genetic Resources of Mongolian Plateau, Inner Mongolia University, Huhhot 010021, China Research Center for Animal Genetic Resources of Mongolian Plateau, Inner Mongolia University, Huhhot 010021, China Research Center for Animal Genetic Resources of Mongolian Plateau, Inner Mongolia University, Huhhot 010021, China Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Huhhot 011517, China Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Huhhot 011517, China Research Center for Animal Genetic Resources of Mongolian Plateau, Inner Mongolia University, Huhhot 010021, China Research Center for Animal Genetic Resources of Mongolian Plateau, Inner Mongolia University, Huhhot 010021, China Research Center for Animal Genetic Resources of Mongolian Plateau, Inner Mongolia University, Huhhot 010021, China Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Huhhot 011517, China Research Center for Animal Genetic Resources of Mongolian Plateau, Inner Mongolia University, Huhhot 010021, China Research Center for Animal Genetic Resources of Mongolian Plateau, Inner Mongolia University, Huhhot 010021, China Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Huhhot 011517, China Research Center for Animal Genetic Resources of Mongolian Plateau, Inner Mongolia University, Huhhot 010021, China Research Center for Animal Genetic Resources of Mongolian Plateau, Inner Mongolia University, Huhhot 010021, ChinaMost hinnies (female donkey×male horse) and mules (female horse×male donkey) are sterile with few reports of equine fertile hybrids. The main cause of this sterility is thought to be a meiotic block to spermatogenesis and oogenesis. This study compared the developmental features of the testes and a histological analyses of spermatogenesis in a male hinny with those of a normal, fertile stallion and Jack donkey. Hinny testes showed a thicker tunica albuginea, fewer blood vessels and more connective tissue in the testis parenchyma than those of the stallion and Jack donkey. Although the mean number of seminiferous tubules was significantly higher in stallion and hinny than Jack donkey (p<0.01), the mean proportion of seminiferous tubules was lower in the hinny (p<0.01) which resulted in a smaller diameter of seminiferous tubules. The mean number of spermatogonia and spermatocytes per unit area were significantly lower in hinny testis (p<0.01) and no spermatids or mature spermatozoa cells were found during immunofluorescent analyses. These results indicated that defects in seminiferous tubule development and structure occur in the testis of hinnies. Furthermore, most spermatogonia and spermatocytes cease development in synapsis during mid-meiosis of spermatocytes, which results in a block to spermatogenesis that prevents the formation of spermatids and matured spermatozoa during meiosis in male hinnies.http://www.ajas.info/upload/pdf/ajas-29-6-793.pdfHinnyTestisSeminiferous TubuleSpermatogenesisDefectSterility |
| spellingShingle | Hongmei Han Aihong Wang Liming Liu Gaoping Zhao Jie Su Biao Wang Yunxia Li Jindun Zhang Baojiang Wu Wei Sun Shuxiang Hu Shuyu Li Lixia Zhao Xihe Li Testicular Characteristics and the Block to Spermatogenesis in Mature Hinny Asian-Australasian Journal of Animal Sciences Hinny Testis Seminiferous Tubule Spermatogenesis Defect Sterility |
| title | Testicular Characteristics and the Block to Spermatogenesis in Mature Hinny |
| title_full | Testicular Characteristics and the Block to Spermatogenesis in Mature Hinny |
| title_fullStr | Testicular Characteristics and the Block to Spermatogenesis in Mature Hinny |
| title_full_unstemmed | Testicular Characteristics and the Block to Spermatogenesis in Mature Hinny |
| title_short | Testicular Characteristics and the Block to Spermatogenesis in Mature Hinny |
| title_sort | testicular characteristics and the block to spermatogenesis in mature hinny |
| topic | Hinny Testis Seminiferous Tubule Spermatogenesis Defect Sterility |
| url | http://www.ajas.info/upload/pdf/ajas-29-6-793.pdf |
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