Association between eNOS gene promoter polymorphism (-786T>C) and idiopathic recurrent pregnancy loss in Iranian women
Objective: To investigate the frequency of -786T>C variant in endothelial nitric oxide synthase (eNOS) gene promoter in Iranian women with recurrent pregnancy loss. Methods: Blood samples were obtained from 100 unrelated women affected by recurrent pregnancy loss and 100 unaffected women as the c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Asian Pacific Journal of Reproduction |
| Subjects: | |
| Online Access: | http://www.apjr.net/article.asp?issn=2305-0500;year=2021;volume=10;issue=6;spage=269;epage=273;aulast=Jalili |
| _version_ | 1818837975473061888 |
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| author | Maryam Sadat Jalili Samira Asadollahi Seyed Morteza Seifati Hamid Reza Ashrafzadeh Nasrin Ghasemi |
| author_facet | Maryam Sadat Jalili Samira Asadollahi Seyed Morteza Seifati Hamid Reza Ashrafzadeh Nasrin Ghasemi |
| author_sort | Maryam Sadat Jalili |
| collection | DOAJ |
| description | Objective: To investigate the frequency of -786T>C variant in endothelial nitric oxide synthase (eNOS) gene promoter in Iranian women with recurrent pregnancy loss.
Methods: Blood samples were obtained from 100 unrelated women affected by recurrent pregnancy loss and 100 unaffected women as the controls. Genomic DNA was extracted and -786T>C polymorphism in eNOS gene promoter was investigated by PCR-RFLP method. Statistical analyses and Hardy-Weinberg equilibrium in the groups of patients and controls were performed by Chi-square test and SPSS standard software (Version 21).
Results: The frequency of homozygous TT was 40% in cases and 46% in the control group; the frequency of CC was 7% in cases and 5% in the control group; frequency heterozygote TC was 53% in cases and 49% in the control group. Genotype frequencies between the two groups showed no significant differences (P>0.05).
Conclusions: The -786T>C polymorphism is not more frequent in recurrent pregnancy loss in this population. |
| first_indexed | 2024-12-19T03:31:02Z |
| format | Article |
| id | doaj.art-ef0ff91c99634dee978764e2830eebd0 |
| institution | Directory Open Access Journal |
| issn | 2305-0500 2305-0519 |
| language | English |
| last_indexed | 2024-12-19T03:31:02Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Reproduction |
| spelling | doaj.art-ef0ff91c99634dee978764e2830eebd02022-12-21T20:37:30ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Reproduction2305-05002305-05192021-01-0110626927310.4103/2305-0500.331264Association between eNOS gene promoter polymorphism (-786T>C) and idiopathic recurrent pregnancy loss in Iranian womenMaryam Sadat JaliliSamira AsadollahiSeyed Morteza SeifatiHamid Reza AshrafzadehNasrin GhasemiObjective: To investigate the frequency of -786T>C variant in endothelial nitric oxide synthase (eNOS) gene promoter in Iranian women with recurrent pregnancy loss. Methods: Blood samples were obtained from 100 unrelated women affected by recurrent pregnancy loss and 100 unaffected women as the controls. Genomic DNA was extracted and -786T>C polymorphism in eNOS gene promoter was investigated by PCR-RFLP method. Statistical analyses and Hardy-Weinberg equilibrium in the groups of patients and controls were performed by Chi-square test and SPSS standard software (Version 21). Results: The frequency of homozygous TT was 40% in cases and 46% in the control group; the frequency of CC was 7% in cases and 5% in the control group; frequency heterozygote TC was 53% in cases and 49% in the control group. Genotype frequencies between the two groups showed no significant differences (P>0.05). Conclusions: The -786T>C polymorphism is not more frequent in recurrent pregnancy loss in this population.http://www.apjr.net/article.asp?issn=2305-0500;year=2021;volume=10;issue=6;spage=269;epage=273;aulast=Jalilirepeated pregnancy loss; endothelial nitric oxide synthase; enos; -786t>c variant; pcr-rflp; iranian women |
| spellingShingle | Maryam Sadat Jalili Samira Asadollahi Seyed Morteza Seifati Hamid Reza Ashrafzadeh Nasrin Ghasemi Association between eNOS gene promoter polymorphism (-786T>C) and idiopathic recurrent pregnancy loss in Iranian women Asian Pacific Journal of Reproduction repeated pregnancy loss; endothelial nitric oxide synthase; enos; -786t>c variant; pcr-rflp; iranian women |
| title | Association between eNOS gene promoter polymorphism (-786T>C) and idiopathic recurrent pregnancy loss in Iranian women |
| title_full | Association between eNOS gene promoter polymorphism (-786T>C) and idiopathic recurrent pregnancy loss in Iranian women |
| title_fullStr | Association between eNOS gene promoter polymorphism (-786T>C) and idiopathic recurrent pregnancy loss in Iranian women |
| title_full_unstemmed | Association between eNOS gene promoter polymorphism (-786T>C) and idiopathic recurrent pregnancy loss in Iranian women |
| title_short | Association between eNOS gene promoter polymorphism (-786T>C) and idiopathic recurrent pregnancy loss in Iranian women |
| title_sort | association between enos gene promoter polymorphism 786t c and idiopathic recurrent pregnancy loss in iranian women |
| topic | repeated pregnancy loss; endothelial nitric oxide synthase; enos; -786t>c variant; pcr-rflp; iranian women |
| url | http://www.apjr.net/article.asp?issn=2305-0500;year=2021;volume=10;issue=6;spage=269;epage=273;aulast=Jalili |
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