Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity
Abstract Background Non-thermal atmospheric pressure plasma technologies form the core of many scientific advances, including in the electronic, industrial, and biotechnological fields. The use of plasma as a cancer therapy has recently attracted significant attention due to its cancer cell killing...
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Format: | Article |
Language: | English |
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BMC
2023-01-01
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Series: | Genes and Environment |
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Online Access: | https://doi.org/10.1186/s41021-023-00260-x |
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author | Yang Liu Yoshimichi Nakatsu Hiromasa Tanaka Kazunori Koga Kenji Ishikawa Masaharu Shiratani Masaru Hori |
author_facet | Yang Liu Yoshimichi Nakatsu Hiromasa Tanaka Kazunori Koga Kenji Ishikawa Masaharu Shiratani Masaru Hori |
author_sort | Yang Liu |
collection | DOAJ |
description | Abstract Background Non-thermal atmospheric pressure plasma technologies form the core of many scientific advances, including in the electronic, industrial, and biotechnological fields. The use of plasma as a cancer therapy has recently attracted significant attention due to its cancer cell killing activity. Plasma-activated Ringer’s lactate solution (PAL) exhibits such activity. In addition to ROS, PAL contains active compounds or species that cause cancer cell death, but the potential mutagenic risks of PAL have not been studied. Results PAL has a low pH value and a high concentration of H2O2. H2O2 was removed from PAL using catalase and catalase-treated PAL with a pH of 5.9 retained a killing effect on HeLa cells whereas this effect was not observed if the PAL was adjusted to pH 7.2. Catalase-treated PAL at pH 5.9 had no significant effect on mutation frequency, the expression of γH2AX, or G2 arrest in HeLa cells. Conclusion PAL contains one or more active compounds or species in addition to H2O2 that have a killing effect on HeLa cells. The compound(s) is active at lower pH conditions and apparently exhibits no genotoxicity. This study suggested that identification of the active compound(s) in PAL could lead to the development of novel anticancer drugs for future cancer therapy. |
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format | Article |
id | doaj.art-ef1049a8dd8d4dd19a5466c0a4f71840 |
institution | Directory Open Access Journal |
issn | 1880-7062 |
language | English |
last_indexed | 2024-04-10T22:45:29Z |
publishDate | 2023-01-01 |
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series | Genes and Environment |
spelling | doaj.art-ef1049a8dd8d4dd19a5466c0a4f718402023-01-15T12:21:38ZengBMCGenes and Environment1880-70622023-01-0145111010.1186/s41021-023-00260-xEffects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicityYang Liu0Yoshimichi Nakatsu1Hiromasa Tanaka2Kazunori Koga3Kenji Ishikawa4Masaharu Shiratani5Masaru Hori6Graduate School of Engineering, Nagoya UniversityGraduate School of Medical Sciences, Kyushu UniversityCenter for Low-temperature Plasma Sciences, Nagoya UniversityCenter for Low-temperature Plasma Sciences, Nagoya UniversityCenter for Low-temperature Plasma Sciences, Nagoya UniversityCenter for Low-temperature Plasma Sciences, Nagoya UniversityCenter for Low-temperature Plasma Sciences, Nagoya UniversityAbstract Background Non-thermal atmospheric pressure plasma technologies form the core of many scientific advances, including in the electronic, industrial, and biotechnological fields. The use of plasma as a cancer therapy has recently attracted significant attention due to its cancer cell killing activity. Plasma-activated Ringer’s lactate solution (PAL) exhibits such activity. In addition to ROS, PAL contains active compounds or species that cause cancer cell death, but the potential mutagenic risks of PAL have not been studied. Results PAL has a low pH value and a high concentration of H2O2. H2O2 was removed from PAL using catalase and catalase-treated PAL with a pH of 5.9 retained a killing effect on HeLa cells whereas this effect was not observed if the PAL was adjusted to pH 7.2. Catalase-treated PAL at pH 5.9 had no significant effect on mutation frequency, the expression of γH2AX, or G2 arrest in HeLa cells. Conclusion PAL contains one or more active compounds or species in addition to H2O2 that have a killing effect on HeLa cells. The compound(s) is active at lower pH conditions and apparently exhibits no genotoxicity. This study suggested that identification of the active compound(s) in PAL could lead to the development of novel anticancer drugs for future cancer therapy.https://doi.org/10.1186/s41021-023-00260-xPALGenotoxicityCytotoxicity |
spellingShingle | Yang Liu Yoshimichi Nakatsu Hiromasa Tanaka Kazunori Koga Kenji Ishikawa Masaharu Shiratani Masaru Hori Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity Genes and Environment PAL Genotoxicity Cytotoxicity |
title | Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity |
title_full | Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity |
title_fullStr | Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity |
title_full_unstemmed | Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity |
title_short | Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity |
title_sort | effects of plasma activated ringer s lactate solution on cancer cells evaluation of genotoxicity |
topic | PAL Genotoxicity Cytotoxicity |
url | https://doi.org/10.1186/s41021-023-00260-x |
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