Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity

Abstract Background Non-thermal atmospheric pressure plasma technologies form the core of many scientific advances, including in the electronic, industrial, and biotechnological fields. The use of plasma as a cancer therapy has recently attracted significant attention due to its cancer cell killing...

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Main Authors: Yang Liu, Yoshimichi Nakatsu, Hiromasa Tanaka, Kazunori Koga, Kenji Ishikawa, Masaharu Shiratani, Masaru Hori
Format: Article
Language:English
Published: BMC 2023-01-01
Series:Genes and Environment
Subjects:
Online Access:https://doi.org/10.1186/s41021-023-00260-x
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author Yang Liu
Yoshimichi Nakatsu
Hiromasa Tanaka
Kazunori Koga
Kenji Ishikawa
Masaharu Shiratani
Masaru Hori
author_facet Yang Liu
Yoshimichi Nakatsu
Hiromasa Tanaka
Kazunori Koga
Kenji Ishikawa
Masaharu Shiratani
Masaru Hori
author_sort Yang Liu
collection DOAJ
description Abstract Background Non-thermal atmospheric pressure plasma technologies form the core of many scientific advances, including in the electronic, industrial, and biotechnological fields. The use of plasma as a cancer therapy has recently attracted significant attention due to its cancer cell killing activity. Plasma-activated Ringer’s lactate solution (PAL) exhibits such activity. In addition to ROS, PAL contains active compounds or species that cause cancer cell death, but the potential mutagenic risks of PAL have not been studied. Results PAL has a low pH value and a high concentration of H2O2. H2O2 was removed from PAL using catalase and catalase-treated PAL with a pH of 5.9 retained a killing effect on HeLa cells whereas this effect was not observed if the PAL was adjusted to pH 7.2. Catalase-treated PAL at pH 5.9 had no significant effect on mutation frequency, the expression of γH2AX, or G2 arrest in HeLa cells. Conclusion PAL contains one or more active compounds or species in addition to H2O2 that have a killing effect on HeLa cells. The compound(s) is active at lower pH conditions and apparently exhibits no genotoxicity. This study suggested that identification of the active compound(s) in PAL could lead to the development of novel anticancer drugs for future cancer therapy.
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spelling doaj.art-ef1049a8dd8d4dd19a5466c0a4f718402023-01-15T12:21:38ZengBMCGenes and Environment1880-70622023-01-0145111010.1186/s41021-023-00260-xEffects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicityYang Liu0Yoshimichi Nakatsu1Hiromasa Tanaka2Kazunori Koga3Kenji Ishikawa4Masaharu Shiratani5Masaru Hori6Graduate School of Engineering, Nagoya UniversityGraduate School of Medical Sciences, Kyushu UniversityCenter for Low-temperature Plasma Sciences, Nagoya UniversityCenter for Low-temperature Plasma Sciences, Nagoya UniversityCenter for Low-temperature Plasma Sciences, Nagoya UniversityCenter for Low-temperature Plasma Sciences, Nagoya UniversityCenter for Low-temperature Plasma Sciences, Nagoya UniversityAbstract Background Non-thermal atmospheric pressure plasma technologies form the core of many scientific advances, including in the electronic, industrial, and biotechnological fields. The use of plasma as a cancer therapy has recently attracted significant attention due to its cancer cell killing activity. Plasma-activated Ringer’s lactate solution (PAL) exhibits such activity. In addition to ROS, PAL contains active compounds or species that cause cancer cell death, but the potential mutagenic risks of PAL have not been studied. Results PAL has a low pH value and a high concentration of H2O2. H2O2 was removed from PAL using catalase and catalase-treated PAL with a pH of 5.9 retained a killing effect on HeLa cells whereas this effect was not observed if the PAL was adjusted to pH 7.2. Catalase-treated PAL at pH 5.9 had no significant effect on mutation frequency, the expression of γH2AX, or G2 arrest in HeLa cells. Conclusion PAL contains one or more active compounds or species in addition to H2O2 that have a killing effect on HeLa cells. The compound(s) is active at lower pH conditions and apparently exhibits no genotoxicity. This study suggested that identification of the active compound(s) in PAL could lead to the development of novel anticancer drugs for future cancer therapy.https://doi.org/10.1186/s41021-023-00260-xPALGenotoxicityCytotoxicity
spellingShingle Yang Liu
Yoshimichi Nakatsu
Hiromasa Tanaka
Kazunori Koga
Kenji Ishikawa
Masaharu Shiratani
Masaru Hori
Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity
Genes and Environment
PAL
Genotoxicity
Cytotoxicity
title Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity
title_full Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity
title_fullStr Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity
title_full_unstemmed Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity
title_short Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity
title_sort effects of plasma activated ringer s lactate solution on cancer cells evaluation of genotoxicity
topic PAL
Genotoxicity
Cytotoxicity
url https://doi.org/10.1186/s41021-023-00260-x
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