Assessment of DNA Methylation and Oxidative Changes in the Heart and Brain of Rats Receiving a High-Fat Diet Supplemented with Various Forms of Chromium
The aim of the study was to determine how feeding rats a high-fat diet supplemented with various forms of chromium affects DNA methylation and oxidation reactions as well as the histology of heart and brain tissue. The rats received standard diet or high-fat diet and chromium at 0.3 mg/kg body weigh...
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MDPI AG
2020-08-01
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| Online Access: | https://www.mdpi.com/2076-2615/10/9/1470 |
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| author | Wojciech Dworzański Ewelina Cholewińska Bartosz Fotschki Jerzy Juśkiewicz Piotr Listos Katarzyna Ognik |
| author_facet | Wojciech Dworzański Ewelina Cholewińska Bartosz Fotschki Jerzy Juśkiewicz Piotr Listos Katarzyna Ognik |
| author_sort | Wojciech Dworzański |
| collection | DOAJ |
| description | The aim of the study was to determine how feeding rats a high-fat diet supplemented with various forms of chromium affects DNA methylation and oxidation reactions as well as the histology of heart and brain tissue. The rats received standard diet or high-fat diet and chromium at 0.3 mg/kg body weight (BW) in form of chromium (III) picolinate, chromium (III)-methionine, or nano-sized chromium. The content of malondialdehyde (MDA), protein carbonyl (PC), and 8-hydroxydeoxyguanosine (8-OHDG), the level of global DNA methylation and the activity of selected DNA repair enzymes were determined in the blood. In the brain and heart, the content of MDA, PC, 8-OHDG, and levels of global DNA methylation were determined. The brain was subjected to histological examination. The use of a high-fat diet was found to intensify epigenetic changes and oxidation reactions in the heart and brain. It was concluded that epigenetic changes and oxidation of lipids, proteins, and DNA in the heart and brain of rats resulting from the use of a high-fat diet cannot be limited by supplementing the diet with chromium. It was established that the use of chromium to supplement a high-fat diet intensifies the negative epigenetic and oxidative changes in the heart and brain, especially in the case of chromium nanoparticles. |
| first_indexed | 2024-03-10T17:03:25Z |
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| id | doaj.art-ef104da613784ef6b6ce056ce13baf1f |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T17:03:25Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ef104da613784ef6b6ce056ce13baf1f2023-11-20T10:54:26ZengMDPI AGAnimals2076-26152020-08-01109147010.3390/ani10091470Assessment of DNA Methylation and Oxidative Changes in the Heart and Brain of Rats Receiving a High-Fat Diet Supplemented with Various Forms of ChromiumWojciech Dworzański0Ewelina Cholewińska1Bartosz Fotschki2Jerzy Juśkiewicz3Piotr Listos4Katarzyna Ognik5Chair and Department of Human Anatomy, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, PolandDepartment of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, PolandAnimal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10–748 Olsztyn, PolandAnimal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10–748 Olsztyn, PolandSub-Department of Pathomorphology and Forensic Medicine, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, PolandDepartment of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, PolandThe aim of the study was to determine how feeding rats a high-fat diet supplemented with various forms of chromium affects DNA methylation and oxidation reactions as well as the histology of heart and brain tissue. The rats received standard diet or high-fat diet and chromium at 0.3 mg/kg body weight (BW) in form of chromium (III) picolinate, chromium (III)-methionine, or nano-sized chromium. The content of malondialdehyde (MDA), protein carbonyl (PC), and 8-hydroxydeoxyguanosine (8-OHDG), the level of global DNA methylation and the activity of selected DNA repair enzymes were determined in the blood. In the brain and heart, the content of MDA, PC, 8-OHDG, and levels of global DNA methylation were determined. The brain was subjected to histological examination. The use of a high-fat diet was found to intensify epigenetic changes and oxidation reactions in the heart and brain. It was concluded that epigenetic changes and oxidation of lipids, proteins, and DNA in the heart and brain of rats resulting from the use of a high-fat diet cannot be limited by supplementing the diet with chromium. It was established that the use of chromium to supplement a high-fat diet intensifies the negative epigenetic and oxidative changes in the heart and brain, especially in the case of chromium nanoparticles.https://www.mdpi.com/2076-2615/10/9/1470oxidative stressepigenetic changesDNA repair enzymeschromium picolinatechromium (III)-methioninechromium nanoparticles |
| spellingShingle | Wojciech Dworzański Ewelina Cholewińska Bartosz Fotschki Jerzy Juśkiewicz Piotr Listos Katarzyna Ognik Assessment of DNA Methylation and Oxidative Changes in the Heart and Brain of Rats Receiving a High-Fat Diet Supplemented with Various Forms of Chromium Animals oxidative stress epigenetic changes DNA repair enzymes chromium picolinate chromium (III)-methionine chromium nanoparticles |
| title | Assessment of DNA Methylation and Oxidative Changes in the Heart and Brain of Rats Receiving a High-Fat Diet Supplemented with Various Forms of Chromium |
| title_full | Assessment of DNA Methylation and Oxidative Changes in the Heart and Brain of Rats Receiving a High-Fat Diet Supplemented with Various Forms of Chromium |
| title_fullStr | Assessment of DNA Methylation and Oxidative Changes in the Heart and Brain of Rats Receiving a High-Fat Diet Supplemented with Various Forms of Chromium |
| title_full_unstemmed | Assessment of DNA Methylation and Oxidative Changes in the Heart and Brain of Rats Receiving a High-Fat Diet Supplemented with Various Forms of Chromium |
| title_short | Assessment of DNA Methylation and Oxidative Changes in the Heart and Brain of Rats Receiving a High-Fat Diet Supplemented with Various Forms of Chromium |
| title_sort | assessment of dna methylation and oxidative changes in the heart and brain of rats receiving a high fat diet supplemented with various forms of chromium |
| topic | oxidative stress epigenetic changes DNA repair enzymes chromium picolinate chromium (III)-methionine chromium nanoparticles |
| url | https://www.mdpi.com/2076-2615/10/9/1470 |
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