Accurate identification and epidemiological characterization of Burkholderia cepacia complex: an update
Abstract Bacteria belonging to the Burkholderia cepacia complex (Bcc) are among the most important pathogens isolated from cystic fibrosis (CF) patients and in hospital acquired infections (HAI). Accurate identification of Bcc is questionable by conventional biochemical methods. Clonal typing of Bur...
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| Format: | Article |
| Language: | English |
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BMC
2019-02-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12941-019-0306-0 |
| _version_ | 1828793001076326400 |
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| author | Naveen Kumar Devanga Ragupathi Balaji Veeraraghavan |
| author_facet | Naveen Kumar Devanga Ragupathi Balaji Veeraraghavan |
| author_sort | Naveen Kumar Devanga Ragupathi |
| collection | DOAJ |
| description | Abstract Bacteria belonging to the Burkholderia cepacia complex (Bcc) are among the most important pathogens isolated from cystic fibrosis (CF) patients and in hospital acquired infections (HAI). Accurate identification of Bcc is questionable by conventional biochemical methods. Clonal typing of Burkholderia is also limited due to the problem with identification. Phenotypic identification methods such as VITEK2, protein signature identification methods like VITEK MS, Bruker Biotyper, and molecular targets such as 16S rRNA, recA, hisA and rpsU were reported with varying level of discrimination to identify Bcc. rpsU and/or 16S rRNA sequencing, VITEK2, VITEK MS and Bruker Biotyper could discriminate between Burkholderia spp. and non-Burkholderia spp. Whereas, Bcc complex level identification can be given by VITEK MS, Bruker Biotyper, and 16S rRNA/rpsU/recA/hisA sequencing. For species level identification within Bcc hisA or recA sequencing are reliable. Identification of Bcc is indispensable in CF patients and HAI to ensure appropriate antimicrobial therapy. |
| first_indexed | 2024-12-12T03:19:47Z |
| format | Article |
| id | doaj.art-ef12020390ab409e9d352bb6a1349ff6 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-12-12T03:19:47Z |
| publishDate | 2019-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-ef12020390ab409e9d352bb6a1349ff62022-12-22T00:40:12ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112019-02-0118111010.1186/s12941-019-0306-0Accurate identification and epidemiological characterization of Burkholderia cepacia complex: an updateNaveen Kumar Devanga Ragupathi0Balaji Veeraraghavan1Department of Clinical Microbiology, Christian Medical CollegeDepartment of Clinical Microbiology, Christian Medical CollegeAbstract Bacteria belonging to the Burkholderia cepacia complex (Bcc) are among the most important pathogens isolated from cystic fibrosis (CF) patients and in hospital acquired infections (HAI). Accurate identification of Bcc is questionable by conventional biochemical methods. Clonal typing of Burkholderia is also limited due to the problem with identification. Phenotypic identification methods such as VITEK2, protein signature identification methods like VITEK MS, Bruker Biotyper, and molecular targets such as 16S rRNA, recA, hisA and rpsU were reported with varying level of discrimination to identify Bcc. rpsU and/or 16S rRNA sequencing, VITEK2, VITEK MS and Bruker Biotyper could discriminate between Burkholderia spp. and non-Burkholderia spp. Whereas, Bcc complex level identification can be given by VITEK MS, Bruker Biotyper, and 16S rRNA/rpsU/recA/hisA sequencing. For species level identification within Bcc hisA or recA sequencing are reliable. Identification of Bcc is indispensable in CF patients and HAI to ensure appropriate antimicrobial therapy.http://link.springer.com/article/10.1186/s12941-019-0306-0Burkholderia cepacia complexCystic fibrosisHospital acquired infectionsrecAhisArpsU |
| spellingShingle | Naveen Kumar Devanga Ragupathi Balaji Veeraraghavan Accurate identification and epidemiological characterization of Burkholderia cepacia complex: an update Annals of Clinical Microbiology and Antimicrobials Burkholderia cepacia complex Cystic fibrosis Hospital acquired infections recA hisA rpsU |
| title | Accurate identification and epidemiological characterization of Burkholderia cepacia complex: an update |
| title_full | Accurate identification and epidemiological characterization of Burkholderia cepacia complex: an update |
| title_fullStr | Accurate identification and epidemiological characterization of Burkholderia cepacia complex: an update |
| title_full_unstemmed | Accurate identification and epidemiological characterization of Burkholderia cepacia complex: an update |
| title_short | Accurate identification and epidemiological characterization of Burkholderia cepacia complex: an update |
| title_sort | accurate identification and epidemiological characterization of burkholderia cepacia complex an update |
| topic | Burkholderia cepacia complex Cystic fibrosis Hospital acquired infections recA hisA rpsU |
| url | http://link.springer.com/article/10.1186/s12941-019-0306-0 |
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