Real-Time PCR Data Processing Shown by the Analysis of Colorectal Specific Candidate Genes, ERCC1, RRM1 and TS in Relation to β2M as Endogenous Control

Real-Time PCR Data Processing Shown by the Analysis of Colorectal Specific Candidate Genes, ERCC1, RRM1 and TS in Relation to β2M as Endogenous Control

Currently, quantitative real-time PCR (Q-PCR) of archival formalin-fixed, paraffin embedded (FFPE) tissue is a critical tool for research and is not well established in routine diagnostics. Therefore, continuous improvement in mathematics and statistics associated with interpreting final accurate an...

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Bibliographic Details
Main Authors: Melanie Demes, Holger Bartsch, Stefanie Scheil-Bertram, Ralph Mücke, Annette Fisseler-Eckhoff
Format: Article
Language:English
Published: MDPI AG 2012-02-01
Series:Applied Sciences
Subjects:
β2M
ERCC1
Q-PCR-Efficiency
RRM1
TYMS
Online Access:http://www.mdpi.com/2076-3417/2/1/139

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