Beneficial Effects of Gosha-jinki-gan and Green Tea Extract in Rats With Chemical Cystitis
The aim of this study was to characterize pharmacological effects of gosha-jinki-gan (GJG) and green tea extract (GTE), on urodynamic parameters, bladder receptors, and urinary cytokines in rats with cyclophosphamide (CYP)-induced cystitis. Urodynamic parameters in CYP-treated rats were measured usi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2013-01-01
|
| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S134786131930297X |
| _version_ | 1819115811508322304 |
|---|---|
| author | Sweety Nasrin Ayaka Osano Yoshihiko Ito Shizuo Yamada |
| author_facet | Sweety Nasrin Ayaka Osano Yoshihiko Ito Shizuo Yamada |
| author_sort | Sweety Nasrin |
| collection | DOAJ |
| description | The aim of this study was to characterize pharmacological effects of gosha-jinki-gan (GJG) and green tea extract (GTE), on urodynamic parameters, bladder receptors, and urinary cytokines in rats with cyclophosphamide (CYP)-induced cystitis. Urodynamic parameters in CYP-treated rats were measured using the cystometric method. Muscarinic and purinergic receptors in rat tissues were measured by radioreceptor assays. Urinary cytokine levels were measured with ELISA kits. GJG and GTE were orally administered to rats once a day for 7 days. The GJG treatment significantly ameliorated changes in urodynamic parameters in CYP-treated rats. Similar treatment with GTE slightly attenuated changes in urodynamic parameters. The maximal number of binding sites for [3H]NMS and [3H]αβ-MeATP in the bladder was significantly lower in CYP-treated rats than in sham rats. Such a reduction in receptor density was significantly attenuated by the GJG treatment. GTE treatment also significantly attenuated the down-regulation of muscarinic receptors, but not P2X receptors in bladders of rats with CYP-induced cystitis. The elevation in urinary cytokine levels in CYP-treated rats was effectively attenuated by GJG treatment. The elevation in cytokine levels in CYP-treated rats was alleviated by GTE treatment. In conclusion, GJG may be a pharmacologically useful plant extract for cystitis. Keywords:: plant extract, muscarinic and purinergic receptor, cyclophosphamide, cystitis, urinary cytokine |
| first_indexed | 2024-12-22T05:07:07Z |
| format | Article |
| id | doaj.art-ef221e5182654ec59df5c94b4c107833 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-22T05:07:07Z |
| publishDate | 2013-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-ef221e5182654ec59df5c94b4c1078332022-12-21T18:38:04ZengElsevierJournal of Pharmacological Sciences1347-86132013-01-011224270277Beneficial Effects of Gosha-jinki-gan and Green Tea Extract in Rats With Chemical CystitisSweety Nasrin0Ayaka Osano1Yoshihiko Ito2Shizuo Yamada3Department of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, JapanDepartment of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, JapanDepartment of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, JapanDepartment of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan; Corresponding author. yamada@u-shizuoka-ken.ac.jpThe aim of this study was to characterize pharmacological effects of gosha-jinki-gan (GJG) and green tea extract (GTE), on urodynamic parameters, bladder receptors, and urinary cytokines in rats with cyclophosphamide (CYP)-induced cystitis. Urodynamic parameters in CYP-treated rats were measured using the cystometric method. Muscarinic and purinergic receptors in rat tissues were measured by radioreceptor assays. Urinary cytokine levels were measured with ELISA kits. GJG and GTE were orally administered to rats once a day for 7 days. The GJG treatment significantly ameliorated changes in urodynamic parameters in CYP-treated rats. Similar treatment with GTE slightly attenuated changes in urodynamic parameters. The maximal number of binding sites for [3H]NMS and [3H]αβ-MeATP in the bladder was significantly lower in CYP-treated rats than in sham rats. Such a reduction in receptor density was significantly attenuated by the GJG treatment. GTE treatment also significantly attenuated the down-regulation of muscarinic receptors, but not P2X receptors in bladders of rats with CYP-induced cystitis. The elevation in urinary cytokine levels in CYP-treated rats was effectively attenuated by GJG treatment. The elevation in cytokine levels in CYP-treated rats was alleviated by GTE treatment. In conclusion, GJG may be a pharmacologically useful plant extract for cystitis. Keywords:: plant extract, muscarinic and purinergic receptor, cyclophosphamide, cystitis, urinary cytokinehttp://www.sciencedirect.com/science/article/pii/S134786131930297X |
| spellingShingle | Sweety Nasrin Ayaka Osano Yoshihiko Ito Shizuo Yamada Beneficial Effects of Gosha-jinki-gan and Green Tea Extract in Rats With Chemical Cystitis Journal of Pharmacological Sciences |
| title | Beneficial Effects of Gosha-jinki-gan and Green Tea Extract in Rats With Chemical Cystitis |
| title_full | Beneficial Effects of Gosha-jinki-gan and Green Tea Extract in Rats With Chemical Cystitis |
| title_fullStr | Beneficial Effects of Gosha-jinki-gan and Green Tea Extract in Rats With Chemical Cystitis |
| title_full_unstemmed | Beneficial Effects of Gosha-jinki-gan and Green Tea Extract in Rats With Chemical Cystitis |
| title_short | Beneficial Effects of Gosha-jinki-gan and Green Tea Extract in Rats With Chemical Cystitis |
| title_sort | beneficial effects of gosha jinki gan and green tea extract in rats with chemical cystitis |
| url | http://www.sciencedirect.com/science/article/pii/S134786131930297X |
| work_keys_str_mv | AT sweetynasrin beneficialeffectsofgoshajinkiganandgreenteaextractinratswithchemicalcystitis AT ayakaosano beneficialeffectsofgoshajinkiganandgreenteaextractinratswithchemicalcystitis AT yoshihikoito beneficialeffectsofgoshajinkiganandgreenteaextractinratswithchemicalcystitis AT shizuoyamada beneficialeffectsofgoshajinkiganandgreenteaextractinratswithchemicalcystitis |