Off-target effects in CRISPR/Cas9 gene editing
Gene editing stands for the methods to precisely make changes to a specific nucleic acid sequence. With the recent development of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, gene editing has become efficient, convenient and programmable, leading to promising t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1143157/full |
| _version_ | 1811157847330783232 |
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| author | Congting Guo Congting Guo Xiaoteng Ma Fei Gao Yuxuan Guo Yuxuan Guo Yuxuan Guo Yuxuan Guo |
| author_facet | Congting Guo Congting Guo Xiaoteng Ma Fei Gao Yuxuan Guo Yuxuan Guo Yuxuan Guo Yuxuan Guo |
| author_sort | Congting Guo |
| collection | DOAJ |
| description | Gene editing stands for the methods to precisely make changes to a specific nucleic acid sequence. With the recent development of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, gene editing has become efficient, convenient and programmable, leading to promising translational studies and clinical trials for both genetic and non-genetic diseases. A major concern in the applications of the CRISPR/Cas9 system is about its off-target effects, namely the deposition of unexpected, unwanted, or even adverse alterations to the genome. To date, many methods have been developed to nominate or detect the off-target sites of CRISPR/Cas9, which laid the basis for the successful upgrades of CRISPR/Cas9 derivatives with enhanced precision. In this review, we summarize these technological advancements and discuss about the current challenges in the management of off-target effects for future gene therapy. |
| first_indexed | 2024-04-10T05:13:14Z |
| format | Article |
| id | doaj.art-ef24fdada7f846b1afbab64c81655a9b |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-10T05:13:14Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-ef24fdada7f846b1afbab64c81655a9b2023-03-09T06:11:52ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-03-011110.3389/fbioe.2023.11431571143157Off-target effects in CRISPR/Cas9 gene editingCongting Guo0Congting Guo1Xiaoteng Ma2Fei Gao3Yuxuan Guo4Yuxuan Guo5Yuxuan Guo6Yuxuan Guo7School of Basic Medical Sciences, Peking University Health Science Center, Beijing, ChinaPeking University Institute of Cardiovascular Sciences, Beijing, ChinaDepartment of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, ChinaDepartment of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, ChinaSchool of Basic Medical Sciences, Peking University Health Science Center, Beijing, ChinaPeking University Institute of Cardiovascular Sciences, Beijing, ChinaMinistry of Education Key Laboratory of Molecular Cardiovascular Science, Beijing, ChinaBeijing Key Laboratory of Cardiovascular Receptors Research, Beijing, ChinaGene editing stands for the methods to precisely make changes to a specific nucleic acid sequence. With the recent development of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, gene editing has become efficient, convenient and programmable, leading to promising translational studies and clinical trials for both genetic and non-genetic diseases. A major concern in the applications of the CRISPR/Cas9 system is about its off-target effects, namely the deposition of unexpected, unwanted, or even adverse alterations to the genome. To date, many methods have been developed to nominate or detect the off-target sites of CRISPR/Cas9, which laid the basis for the successful upgrades of CRISPR/Cas9 derivatives with enhanced precision. In this review, we summarize these technological advancements and discuss about the current challenges in the management of off-target effects for future gene therapy.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1143157/fulloff-target effectsgene editingCRISPR/Cas9gene therapyCas9/sgRNA complex |
| spellingShingle | Congting Guo Congting Guo Xiaoteng Ma Fei Gao Yuxuan Guo Yuxuan Guo Yuxuan Guo Yuxuan Guo Off-target effects in CRISPR/Cas9 gene editing Frontiers in Bioengineering and Biotechnology off-target effects gene editing CRISPR/Cas9 gene therapy Cas9/sgRNA complex |
| title | Off-target effects in CRISPR/Cas9 gene editing |
| title_full | Off-target effects in CRISPR/Cas9 gene editing |
| title_fullStr | Off-target effects in CRISPR/Cas9 gene editing |
| title_full_unstemmed | Off-target effects in CRISPR/Cas9 gene editing |
| title_short | Off-target effects in CRISPR/Cas9 gene editing |
| title_sort | off target effects in crispr cas9 gene editing |
| topic | off-target effects gene editing CRISPR/Cas9 gene therapy Cas9/sgRNA complex |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1143157/full |
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