Identification of Endogenous Genes for Normalizing Titer Variation of Citrus Tristeza Virus in Aphids at Different Post-acquisition Feeding Times
Citrus tristeza virus (CTV) is efficiently transmitted in a semi-persistent manner by the brown citrus aphid (Toxoptera citricida (Kirkaldy)). Currently, the most sensitive method for detecting plant viruses in insect vectors is reverse-transcription quantitative polymerase chain reaction (RT-qPCR)....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2022-08-01
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| Series: | The Plant Pathology Journal |
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| Online Access: | http://www.ppjonline.org/upload/pdf/PPJ-OA-01-2022-0011.pdf |
| _version_ | 1811214695385792512 |
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| author | Hongsu Wang Qi Chen Luqin Liu Yan Zhou Huanhuan Wang Zhongan Li Jinxiang Liu |
| author_facet | Hongsu Wang Qi Chen Luqin Liu Yan Zhou Huanhuan Wang Zhongan Li Jinxiang Liu |
| author_sort | Hongsu Wang |
| collection | DOAJ |
| description | Citrus tristeza virus (CTV) is efficiently transmitted in a semi-persistent manner by the brown citrus aphid (Toxoptera citricida (Kirkaldy)). Currently, the most sensitive method for detecting plant viruses in insect vectors is reverse-transcription quantitative polymerase chain reaction (RT-qPCR). In this study, the elongation factor-1 alpha (EF-1α) gene and acidic p0 ribosomal protein (RPAP0) gene were confirmed to be suitable reference genes for RT-qPCR normalization in viruliferous T. citricida aphids using the geNorm, NormFinder, and BestKeeper tools. Then the relative CTV titer in aphids (T. citricida) at different post-acquisition feeding times on healthy plants was quantified by RT-qPCR using EF-1α and RPAP0 as reference genes. The relative CTV titer retained in the aphids gradually decreased with increasing feeding time. During the first 0.5 h of feeding time on healthy plants, the remaining CTV titer in aphids showed about 80% rapid loss for the highly transmissible isolate CT11A and 40% loss for the poorly transmissible isolate CTLJ. The relative CTV titer in aphids during more than 12 h post-acquisition times for CT11A was significantly lower than at the other feeding times, which is similar to the trend found for CTLJ. To our knowledge, this is the first report about the relative titer variation of CTV remaining in T. citricida at different post-acquisition feeding times on healthy plants. |
| first_indexed | 2024-04-12T06:08:57Z |
| format | Article |
| id | doaj.art-ef2902f8cb7a493b8f476537dc3361c9 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-04-12T06:08:57Z |
| publishDate | 2022-08-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-ef2902f8cb7a493b8f476537dc3361c92022-12-22T03:44:46ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802022-08-0138428729510.5423/PPJ.OA.01.2022.00112323Identification of Endogenous Genes for Normalizing Titer Variation of Citrus Tristeza Virus in Aphids at Different Post-acquisition Feeding TimesHongsu Wang0Qi Chen1Luqin Liu2Yan Zhou3Huanhuan Wang4Zhongan Li5Jinxiang Liu6 Citrus Research Institute, Southwest University, Chongqing 400712, China Citrus Research Institute, Southwest University, Chongqing 400712, China Citrus Research Institute, Southwest University, Chongqing 400712, China Citrus Research Institute, Southwest University, Chongqing 400712, China Citrus Research Institute, Southwest University, Chongqing 400712, China Citrus Research Institute, Southwest University, Chongqing 400712, China Citrus Research Institute, Southwest University, Chongqing 400712, ChinaCitrus tristeza virus (CTV) is efficiently transmitted in a semi-persistent manner by the brown citrus aphid (Toxoptera citricida (Kirkaldy)). Currently, the most sensitive method for detecting plant viruses in insect vectors is reverse-transcription quantitative polymerase chain reaction (RT-qPCR). In this study, the elongation factor-1 alpha (EF-1α) gene and acidic p0 ribosomal protein (RPAP0) gene were confirmed to be suitable reference genes for RT-qPCR normalization in viruliferous T. citricida aphids using the geNorm, NormFinder, and BestKeeper tools. Then the relative CTV titer in aphids (T. citricida) at different post-acquisition feeding times on healthy plants was quantified by RT-qPCR using EF-1α and RPAP0 as reference genes. The relative CTV titer retained in the aphids gradually decreased with increasing feeding time. During the first 0.5 h of feeding time on healthy plants, the remaining CTV titer in aphids showed about 80% rapid loss for the highly transmissible isolate CT11A and 40% loss for the poorly transmissible isolate CTLJ. The relative CTV titer in aphids during more than 12 h post-acquisition times for CT11A was significantly lower than at the other feeding times, which is similar to the trend found for CTLJ. To our knowledge, this is the first report about the relative titer variation of CTV remaining in T. citricida at different post-acquisition feeding times on healthy plants.http://www.ppjonline.org/upload/pdf/PPJ-OA-01-2022-0011.pdfcitrus tristeza virusrelative titer variation |
| spellingShingle | Hongsu Wang Qi Chen Luqin Liu Yan Zhou Huanhuan Wang Zhongan Li Jinxiang Liu Identification of Endogenous Genes for Normalizing Titer Variation of Citrus Tristeza Virus in Aphids at Different Post-acquisition Feeding Times The Plant Pathology Journal citrus tristeza virus relative titer variation |
| title | Identification of Endogenous Genes for Normalizing Titer Variation of Citrus Tristeza Virus in Aphids at Different Post-acquisition Feeding Times |
| title_full | Identification of Endogenous Genes for Normalizing Titer Variation of Citrus Tristeza Virus in Aphids at Different Post-acquisition Feeding Times |
| title_fullStr | Identification of Endogenous Genes for Normalizing Titer Variation of Citrus Tristeza Virus in Aphids at Different Post-acquisition Feeding Times |
| title_full_unstemmed | Identification of Endogenous Genes for Normalizing Titer Variation of Citrus Tristeza Virus in Aphids at Different Post-acquisition Feeding Times |
| title_short | Identification of Endogenous Genes for Normalizing Titer Variation of Citrus Tristeza Virus in Aphids at Different Post-acquisition Feeding Times |
| title_sort | identification of endogenous genes for normalizing titer variation of citrus tristeza virus in aphids at different post acquisition feeding times |
| topic | citrus tristeza virus relative titer variation |
| url | http://www.ppjonline.org/upload/pdf/PPJ-OA-01-2022-0011.pdf |
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