Phytophthora capsici sterol reductase PcDHCR7 has a role in mycelium development and pathogenicity
The de novo biosynthesis of sterols is critical for the majority of eukaryotes; however, some organisms lack this pathway, including most oomycetes. Phytophthora spp. are sterol auxotrophic but, remarkably, have retained a few genes encoding enzymes in the sterol biosynthesis pathway. Here, we show...
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| Format: | Article |
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The Royal Society
2022-04-01
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| Series: | Open Biology |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsob.210282 |
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| author | Weizhen Wang Fan Zhang Sicong Zhang Zhaolin Xue Linfang Xie Francine Govers Xili Liu |
| author_facet | Weizhen Wang Fan Zhang Sicong Zhang Zhaolin Xue Linfang Xie Francine Govers Xili Liu |
| author_sort | Weizhen Wang |
| collection | DOAJ |
| description | The de novo biosynthesis of sterols is critical for the majority of eukaryotes; however, some organisms lack this pathway, including most oomycetes. Phytophthora spp. are sterol auxotrophic but, remarkably, have retained a few genes encoding enzymes in the sterol biosynthesis pathway. Here, we show that PcDHCR7, a gene in Phytophthora capsici predicted to encode Δ7-sterol reductase, displays multiple functions. When expressed in Saccharomyces cerevisiae, PcDHCR7 showed the Δ7-sterol reductase activity. Knocking out PcDHCR7 in P. capsici resulted in loss of the capacity to transform ergosterol into brassicasterol, which means PcDHCR7 has the Δ7-sterol reductase activity in P. capsici itself. This enables P. capsici to transform sterols recruited from the environment for better use. The biological characteristics of ΔPcDHCR7 transformants were compared with those of the wild-type strain and a PcDHCR7 complemented transformant, and the results showed that PcDHCR7 plays a key role in mycelium development and pathogenicity of zoospores. Further analysis of the transcriptome indicated that the expression of many genes changed in the ΔPcDHCR7 transformant, which involve in different biological processes. It is possible that P. capsici compensates for the defects caused by the loss of PcDHCR7 by remodelling its transcriptome. |
| first_indexed | 2024-04-09T15:28:33Z |
| format | Article |
| id | doaj.art-ef2aa861cb38442f9053f5331bac85a6 |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-04-09T15:28:33Z |
| publishDate | 2022-04-01 |
| publisher | The Royal Society |
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| series | Open Biology |
| spelling | doaj.art-ef2aa861cb38442f9053f5331bac85a62023-04-28T11:08:27ZengThe Royal SocietyOpen Biology2046-24412022-04-0112410.1098/rsob.210282Phytophthora capsici sterol reductase PcDHCR7 has a role in mycelium development and pathogenicityWeizhen Wang0Fan Zhang1Sicong Zhang2Zhaolin Xue3Linfang Xie4Francine Govers5Xili Liu6Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, People's Republic of ChinaDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, People's Republic of ChinaDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, People's Republic of ChinaDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, People's Republic of ChinaLaboratory of Phytopathology, Wageningen University & Research, Wageningen, The NetherlandsLaboratory of Phytopathology, Wageningen University & Research, Wageningen, The NetherlandsDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, People's Republic of ChinaThe de novo biosynthesis of sterols is critical for the majority of eukaryotes; however, some organisms lack this pathway, including most oomycetes. Phytophthora spp. are sterol auxotrophic but, remarkably, have retained a few genes encoding enzymes in the sterol biosynthesis pathway. Here, we show that PcDHCR7, a gene in Phytophthora capsici predicted to encode Δ7-sterol reductase, displays multiple functions. When expressed in Saccharomyces cerevisiae, PcDHCR7 showed the Δ7-sterol reductase activity. Knocking out PcDHCR7 in P. capsici resulted in loss of the capacity to transform ergosterol into brassicasterol, which means PcDHCR7 has the Δ7-sterol reductase activity in P. capsici itself. This enables P. capsici to transform sterols recruited from the environment for better use. The biological characteristics of ΔPcDHCR7 transformants were compared with those of the wild-type strain and a PcDHCR7 complemented transformant, and the results showed that PcDHCR7 plays a key role in mycelium development and pathogenicity of zoospores. Further analysis of the transcriptome indicated that the expression of many genes changed in the ΔPcDHCR7 transformant, which involve in different biological processes. It is possible that P. capsici compensates for the defects caused by the loss of PcDHCR7 by remodelling its transcriptome.https://royalsocietypublishing.org/doi/10.1098/rsob.210282oomycetePhytophthoraDHCR7sterolreductasemycelium development |
| spellingShingle | Weizhen Wang Fan Zhang Sicong Zhang Zhaolin Xue Linfang Xie Francine Govers Xili Liu Phytophthora capsici sterol reductase PcDHCR7 has a role in mycelium development and pathogenicity Open Biology oomycete Phytophthora DHCR7 sterol reductase mycelium development |
| title | Phytophthora capsici sterol reductase PcDHCR7 has a role in mycelium development and pathogenicity |
| title_full | Phytophthora capsici sterol reductase PcDHCR7 has a role in mycelium development and pathogenicity |
| title_fullStr | Phytophthora capsici sterol reductase PcDHCR7 has a role in mycelium development and pathogenicity |
| title_full_unstemmed | Phytophthora capsici sterol reductase PcDHCR7 has a role in mycelium development and pathogenicity |
| title_short | Phytophthora capsici sterol reductase PcDHCR7 has a role in mycelium development and pathogenicity |
| title_sort | phytophthora capsici sterol reductase pcdhcr7 has a role in mycelium development and pathogenicity |
| topic | oomycete Phytophthora DHCR7 sterol reductase mycelium development |
| url | https://royalsocietypublishing.org/doi/10.1098/rsob.210282 |
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