Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by epistasis acting on ACE2.
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infects a broader range of mammalian species than previously predicted, binding a diversity of angiotensin converting enzyme 2 (ACE2) orthologs despite extensive sequence divergence. Within this sequence degeneracy, we identify a rare sequ...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-12-01
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| Series: | PLoS Biology |
| Online Access: | https://doi.org/10.1371/journal.pbio.3001510 |
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| author | Gianni M Castiglione Lingli Zhou Zhenhua Xu Zachary Neiman Chien-Fu Hung Elia J Duh |
| author_facet | Gianni M Castiglione Lingli Zhou Zhenhua Xu Zachary Neiman Chien-Fu Hung Elia J Duh |
| author_sort | Gianni M Castiglione |
| collection | DOAJ |
| description | Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infects a broader range of mammalian species than previously predicted, binding a diversity of angiotensin converting enzyme 2 (ACE2) orthologs despite extensive sequence divergence. Within this sequence degeneracy, we identify a rare sequence combination capable of conferring SARS-CoV-2 resistance. We demonstrate that this sequence was likely unattainable during human evolution due to deleterious effects on ACE2 carboxypeptidase activity, which has vasodilatory and cardioprotective functions in vivo. Across the 25 ACE2 sites implicated in viral binding, we identify 6 amino acid substitutions unique to mouse-one of the only known mammalian species resistant to SARS-CoV-2. Substituting human variants at these positions is sufficient to confer binding of the SARS-CoV-2 S protein to mouse ACE2, facilitating cellular infection. Conversely, substituting mouse variants into either human or dog ACE2 abolishes viral binding, diminishing cellular infection. However, these same substitutions decrease human ACE2 activity by 50% and are predicted as pathogenic, consistent with the extreme rarity of human polymorphisms at these sites. This trade-off can be avoided, however, depending on genetic background; if substituted simultaneously, these same mutations have no deleterious effect on dog ACE2 nor that of the rodent ancestor estimated to exist 70 million years ago. This genetic contingency (epistasis) may have therefore opened the road to resistance for some species, while making humans susceptible to viruses that use these ACE2 surfaces for binding, as does SARS-CoV-2. |
| first_indexed | 2024-04-11T18:06:59Z |
| format | Article |
| id | doaj.art-ef2cd11c4f3441b2be1b8c76cc620b93 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-04-11T18:06:59Z |
| publishDate | 2021-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-ef2cd11c4f3441b2be1b8c76cc620b932022-12-22T04:10:18ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852021-12-011912e300151010.1371/journal.pbio.3001510Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by epistasis acting on ACE2.Gianni M CastiglioneLingli ZhouZhenhua XuZachary NeimanChien-Fu HungElia J DuhSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infects a broader range of mammalian species than previously predicted, binding a diversity of angiotensin converting enzyme 2 (ACE2) orthologs despite extensive sequence divergence. Within this sequence degeneracy, we identify a rare sequence combination capable of conferring SARS-CoV-2 resistance. We demonstrate that this sequence was likely unattainable during human evolution due to deleterious effects on ACE2 carboxypeptidase activity, which has vasodilatory and cardioprotective functions in vivo. Across the 25 ACE2 sites implicated in viral binding, we identify 6 amino acid substitutions unique to mouse-one of the only known mammalian species resistant to SARS-CoV-2. Substituting human variants at these positions is sufficient to confer binding of the SARS-CoV-2 S protein to mouse ACE2, facilitating cellular infection. Conversely, substituting mouse variants into either human or dog ACE2 abolishes viral binding, diminishing cellular infection. However, these same substitutions decrease human ACE2 activity by 50% and are predicted as pathogenic, consistent with the extreme rarity of human polymorphisms at these sites. This trade-off can be avoided, however, depending on genetic background; if substituted simultaneously, these same mutations have no deleterious effect on dog ACE2 nor that of the rodent ancestor estimated to exist 70 million years ago. This genetic contingency (epistasis) may have therefore opened the road to resistance for some species, while making humans susceptible to viruses that use these ACE2 surfaces for binding, as does SARS-CoV-2.https://doi.org/10.1371/journal.pbio.3001510 |
| spellingShingle | Gianni M Castiglione Lingli Zhou Zhenhua Xu Zachary Neiman Chien-Fu Hung Elia J Duh Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by epistasis acting on ACE2. PLoS Biology |
| title | Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by epistasis acting on ACE2. |
| title_full | Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by epistasis acting on ACE2. |
| title_fullStr | Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by epistasis acting on ACE2. |
| title_full_unstemmed | Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by epistasis acting on ACE2. |
| title_short | Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by epistasis acting on ACE2. |
| title_sort | evolutionary pathways to sars cov 2 resistance are opened and closed by epistasis acting on ace2 |
| url | https://doi.org/10.1371/journal.pbio.3001510 |
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