High strength HA-PEG/NAGA-Gelma double network hydrogel for annulus fibrosus rupture repair
The low back pain (LBP) caused by intervertebral disc (IVD) herniation and degeneration bring huge physical and economic burden to individuals and families. Since current clinical treatment methods could not change the degeneration process of the intervertebral disc, annulus fibrosus (AF) repair as...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-01-01
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| Series: | Smart Materials in Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590183421000508 |
| _version_ | 1811173317588025344 |
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| author | Yichen Zhang Huichang Gao Huitong Luo Dafu Chen Zhiyu Zhou Xiaodong Cao |
| author_facet | Yichen Zhang Huichang Gao Huitong Luo Dafu Chen Zhiyu Zhou Xiaodong Cao |
| author_sort | Yichen Zhang |
| collection | DOAJ |
| description | The low back pain (LBP) caused by intervertebral disc (IVD) herniation and degeneration bring huge physical and economic burden to individuals and families. Since current clinical treatment methods could not change the degeneration process of the intervertebral disc, annulus fibrosus (AF) repair as a method to seal defects of the IVD and prevent reherniation after lumbar discectomy has attracted increasing interest. In this study, a porous HA-PEG/NAGA-GelMA double network hydrogel with the multiple hydrogen bonds was designed and prepared by a two-step method, in which the multiple hydrogen bonds produced by NAGA endowed excellent mechanical properties (compressive strength up to 1.19 MPa and compressive modulus up to 0.55 MPa) to resist the pressure from the AF and the hyaluronic acid provided good biological activity. In vitro cell experiment indicated that the double network hydrogel possessed good cytocompatibility and supported cell proliferation and penetration. In addition, further organ experiments also showed that the double network hydrogel could restore the disc height and mechanical function of the injured intervertebral disc, and display good integration with the host AF, showing a promising application in annulus fibrosus tissue repair after lumbar discectomy. |
| first_indexed | 2024-04-10T17:45:59Z |
| format | Article |
| id | doaj.art-ef371afe601240cb946e4ac1d477cce4 |
| institution | Directory Open Access Journal |
| issn | 2590-1834 |
| language | English |
| last_indexed | 2024-04-10T17:45:59Z |
| publishDate | 2022-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Smart Materials in Medicine |
| spelling | doaj.art-ef371afe601240cb946e4ac1d477cce42023-02-03T05:01:01ZengKeAi Communications Co., Ltd.Smart Materials in Medicine2590-18342022-01-013128138High strength HA-PEG/NAGA-Gelma double network hydrogel for annulus fibrosus rupture repairYichen Zhang0Huichang Gao1Huitong Luo2Dafu Chen3Zhiyu Zhou4Xiaodong Cao5School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, PR China; National Engineering Research Centre for Tissue Restoration and Reconstruction, Guangzhou, 510006, PR ChinaSchool of Medicine, South China University of Technology, Guangzhou, 510006, PR China; National Engineering Research Centre for Tissue Restoration and Reconstruction, Guangzhou, 510006, PR ChinaSchool of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, PR China; National Engineering Research Centre for Tissue Restoration and Reconstruction, Guangzhou, 510006, PR ChinaLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, Beijing Research Institute of Orthopaedics and Traumatology, Beijing JiShuiTan Hospital, Beijing, 100035, PR ChinaInnovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury, Department of Orthopedic Surgery, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, PR China; Guangdong Provincial Key Laboratory of Orthopedics and Traumatology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, PR ChinaSchool of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, PR China; National Engineering Research Centre for Tissue Restoration and Reconstruction, Guangzhou, 510006, PR China; Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou, 510006, PR China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou, 510006, PR China; Corresponding author. School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, PR China.The low back pain (LBP) caused by intervertebral disc (IVD) herniation and degeneration bring huge physical and economic burden to individuals and families. Since current clinical treatment methods could not change the degeneration process of the intervertebral disc, annulus fibrosus (AF) repair as a method to seal defects of the IVD and prevent reherniation after lumbar discectomy has attracted increasing interest. In this study, a porous HA-PEG/NAGA-GelMA double network hydrogel with the multiple hydrogen bonds was designed and prepared by a two-step method, in which the multiple hydrogen bonds produced by NAGA endowed excellent mechanical properties (compressive strength up to 1.19 MPa and compressive modulus up to 0.55 MPa) to resist the pressure from the AF and the hyaluronic acid provided good biological activity. In vitro cell experiment indicated that the double network hydrogel possessed good cytocompatibility and supported cell proliferation and penetration. In addition, further organ experiments also showed that the double network hydrogel could restore the disc height and mechanical function of the injured intervertebral disc, and display good integration with the host AF, showing a promising application in annulus fibrosus tissue repair after lumbar discectomy.http://www.sciencedirect.com/science/article/pii/S2590183421000508annulus fibrosus repairHyaluronic acidDouble network hydrogelSpine biomechanicsIntervertebral disc degeneration |
| spellingShingle | Yichen Zhang Huichang Gao Huitong Luo Dafu Chen Zhiyu Zhou Xiaodong Cao High strength HA-PEG/NAGA-Gelma double network hydrogel for annulus fibrosus rupture repair Smart Materials in Medicine annulus fibrosus repair Hyaluronic acid Double network hydrogel Spine biomechanics Intervertebral disc degeneration |
| title | High strength HA-PEG/NAGA-Gelma double network hydrogel for annulus fibrosus rupture repair |
| title_full | High strength HA-PEG/NAGA-Gelma double network hydrogel for annulus fibrosus rupture repair |
| title_fullStr | High strength HA-PEG/NAGA-Gelma double network hydrogel for annulus fibrosus rupture repair |
| title_full_unstemmed | High strength HA-PEG/NAGA-Gelma double network hydrogel for annulus fibrosus rupture repair |
| title_short | High strength HA-PEG/NAGA-Gelma double network hydrogel for annulus fibrosus rupture repair |
| title_sort | high strength ha peg naga gelma double network hydrogel for annulus fibrosus rupture repair |
| topic | annulus fibrosus repair Hyaluronic acid Double network hydrogel Spine biomechanics Intervertebral disc degeneration |
| url | http://www.sciencedirect.com/science/article/pii/S2590183421000508 |
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