Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma
Basal cell carcinoma (BCC) is the most common skin malignancy worldwide. Current evidence suggests tumour-infiltrating lymphocytes (TILs) may influence the clinical outcomes of patients with BCC. The present study aimed to profile the infiltrative characteristics of stromal TILs and regulatory T cel...
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MDPI AG
2022-11-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/12/2987 |
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| author | Paranita Ferronika Safira Alya Dhiyani Tri Budiarti Irianiwati Widodo Hanggoro Tri Rinonce Sumadi Lukman Anwar |
| author_facet | Paranita Ferronika Safira Alya Dhiyani Tri Budiarti Irianiwati Widodo Hanggoro Tri Rinonce Sumadi Lukman Anwar |
| author_sort | Paranita Ferronika |
| collection | DOAJ |
| description | Basal cell carcinoma (BCC) is the most common skin malignancy worldwide. Current evidence suggests tumour-infiltrating lymphocytes (TILs) may influence the clinical outcomes of patients with BCC. The present study aimed to profile the infiltrative characteristics of stromal TILs and regulatory T cells (Treg cells) in the tumour centre (TC), tumour periphery (TP), and normal adjacent tissue (NAT) of BCC. A total of 111 samples from 43 cutaneous BCC cases were examined for TIL (CD3<sup>+</sup>) and Treg cell (FOXP3<sup>+</sup>/CD3<sup>+</sup>) expression using immunohistochemical techniques. The correlations of Treg cells with TILs, invasion depth, and tumour morphological risk were analysed. We identified a high mean proportion of Treg cells within the tumour (TC = 46.9%, TP = 56.1%, NAT = 51.8%) despite a relatively low median of TILs (TC = 12.7%, TP = 10.3%, NAT = 3.6%), supporting the classification of BCC as a cold tumour. A significant positive correlation was observed between the proportion of Treg cells and sTILs (ρ = 0.325, <i>p <</i> 0.001), suggesting a predominant role of TILs in the infiltration of Treg cells. An inverse correlation discovered between Treg cells and tumour invasion depth (r = −0.36, <i>p =</i> 0.017) might indicate Treg cells’ anti-tumour capacity in BCC. |
| first_indexed | 2024-03-09T17:08:52Z |
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| id | doaj.art-ef39c635847741f3ad2f89781eebdab6 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T17:08:52Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-ef39c635847741f3ad2f89781eebdab62023-11-24T14:16:41ZengMDPI AGDiagnostics2075-44182022-11-011212298710.3390/diagnostics12122987Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell CarcinomaParanita Ferronika0Safira Alya Dhiyani1Tri Budiarti2Irianiwati Widodo3Hanggoro Tri Rinonce4Sumadi Lukman Anwar5Department of Anatomical Pathology, Faculty of Medicine, Public Health and Nursing, Dr. Sardjito Hospital, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Anatomical Pathology, Faculty of Medicine, Public Health and Nursing, Dr. Sardjito Hospital, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Anatomical Pathology, Faculty of Medicine, Public Health and Nursing, Dr. Sardjito Hospital, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Anatomical Pathology, Faculty of Medicine, Public Health and Nursing, Dr. Sardjito Hospital, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Anatomical Pathology, Faculty of Medicine, Public Health and Nursing, Dr. Sardjito Hospital, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Surgery, Faculty of Medicine, Public Health and Nursing, Dr. Sardjito Hospital, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaBasal cell carcinoma (BCC) is the most common skin malignancy worldwide. Current evidence suggests tumour-infiltrating lymphocytes (TILs) may influence the clinical outcomes of patients with BCC. The present study aimed to profile the infiltrative characteristics of stromal TILs and regulatory T cells (Treg cells) in the tumour centre (TC), tumour periphery (TP), and normal adjacent tissue (NAT) of BCC. A total of 111 samples from 43 cutaneous BCC cases were examined for TIL (CD3<sup>+</sup>) and Treg cell (FOXP3<sup>+</sup>/CD3<sup>+</sup>) expression using immunohistochemical techniques. The correlations of Treg cells with TILs, invasion depth, and tumour morphological risk were analysed. We identified a high mean proportion of Treg cells within the tumour (TC = 46.9%, TP = 56.1%, NAT = 51.8%) despite a relatively low median of TILs (TC = 12.7%, TP = 10.3%, NAT = 3.6%), supporting the classification of BCC as a cold tumour. A significant positive correlation was observed between the proportion of Treg cells and sTILs (ρ = 0.325, <i>p <</i> 0.001), suggesting a predominant role of TILs in the infiltration of Treg cells. An inverse correlation discovered between Treg cells and tumour invasion depth (r = −0.36, <i>p =</i> 0.017) might indicate Treg cells’ anti-tumour capacity in BCC.https://www.mdpi.com/2075-4418/12/12/2987basal cell carcinomatumour-infiltrating lymphocyteregulatory T celltumour invasion depth |
| spellingShingle | Paranita Ferronika Safira Alya Dhiyani Tri Budiarti Irianiwati Widodo Hanggoro Tri Rinonce Sumadi Lukman Anwar Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma Diagnostics basal cell carcinoma tumour-infiltrating lymphocyte regulatory T cell tumour invasion depth |
| title | Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma |
| title_full | Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma |
| title_fullStr | Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma |
| title_full_unstemmed | Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma |
| title_short | Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma |
| title_sort | regulatory t cells but not tumour infiltrating lymphocytes correlate with tumour invasion depth in basal cell carcinoma |
| topic | basal cell carcinoma tumour-infiltrating lymphocyte regulatory T cell tumour invasion depth |
| url | https://www.mdpi.com/2075-4418/12/12/2987 |
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