Time-lapse and cleared imaging of mouse embryonic lung explants to study three-dimensional cell morphology and topology dynamics
Summary: Here, we present a protocol for collecting high-spatiotemporal-resolution datasets of undisturbed mouse embryonic epithelial rudiments using light-sheet fluorescence microscopy. We describe steps for rudiment dissection, clearing, and embedding for cleared and live imaging. We then detail p...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723001454 |
| _version_ | 1797862147538026496 |
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| author | Harold Fernando Gómez Nikolaos Doumpas Dagmar Iber |
| author_facet | Harold Fernando Gómez Nikolaos Doumpas Dagmar Iber |
| author_sort | Harold Fernando Gómez |
| collection | DOAJ |
| description | Summary: Here, we present a protocol for collecting high-spatiotemporal-resolution datasets of undisturbed mouse embryonic epithelial rudiments using light-sheet fluorescence microscopy. We describe steps for rudiment dissection, clearing, and embedding for cleared and live imaging. We then detail procedures for light-sheet imaging followed by image processing and morphometric analysis. We provide protocol variations for imaging both growing and optically cleared lung explants to encourage the quantitative exploration of three-dimensional cell shapes, cell organization, and complex cell-cell dynamics.For complete details on the use and execution of this protocol, please refer to Gómez et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-09T22:15:40Z |
| format | Article |
| id | doaj.art-ef3b6eddc36d4912b9813dadc04f3c36 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-09T22:15:40Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-ef3b6eddc36d4912b9813dadc04f3c362023-03-23T04:37:01ZengElsevierSTAR Protocols2666-16672023-06-0142102187Time-lapse and cleared imaging of mouse embryonic lung explants to study three-dimensional cell morphology and topology dynamicsHarold Fernando Gómez0Nikolaos Doumpas1Dagmar Iber2Department of Biosystems Science and Engineering (D-BSSE), ETH Zürich, Basel, Switzerland; Swiss Institute of Bioinformatics (SIB), Basel, Switzerland; Corresponding authorDepartment of Biosystems Science and Engineering (D-BSSE), ETH Zürich, Basel, Switzerland; Swiss Institute of Bioinformatics (SIB), Basel, SwitzerlandDepartment of Biosystems Science and Engineering (D-BSSE), ETH Zürich, Basel, Switzerland; Swiss Institute of Bioinformatics (SIB), Basel, Switzerland; Corresponding authorSummary: Here, we present a protocol for collecting high-spatiotemporal-resolution datasets of undisturbed mouse embryonic epithelial rudiments using light-sheet fluorescence microscopy. We describe steps for rudiment dissection, clearing, and embedding for cleared and live imaging. We then detail procedures for light-sheet imaging followed by image processing and morphometric analysis. We provide protocol variations for imaging both growing and optically cleared lung explants to encourage the quantitative exploration of three-dimensional cell shapes, cell organization, and complex cell-cell dynamics.For complete details on the use and execution of this protocol, please refer to Gómez et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723001454Cell BiologyDevelopmental BiologyMicroscopyModel Organisms |
| spellingShingle | Harold Fernando Gómez Nikolaos Doumpas Dagmar Iber Time-lapse and cleared imaging of mouse embryonic lung explants to study three-dimensional cell morphology and topology dynamics STAR Protocols Cell Biology Developmental Biology Microscopy Model Organisms |
| title | Time-lapse and cleared imaging of mouse embryonic lung explants to study three-dimensional cell morphology and topology dynamics |
| title_full | Time-lapse and cleared imaging of mouse embryonic lung explants to study three-dimensional cell morphology and topology dynamics |
| title_fullStr | Time-lapse and cleared imaging of mouse embryonic lung explants to study three-dimensional cell morphology and topology dynamics |
| title_full_unstemmed | Time-lapse and cleared imaging of mouse embryonic lung explants to study three-dimensional cell morphology and topology dynamics |
| title_short | Time-lapse and cleared imaging of mouse embryonic lung explants to study three-dimensional cell morphology and topology dynamics |
| title_sort | time lapse and cleared imaging of mouse embryonic lung explants to study three dimensional cell morphology and topology dynamics |
| topic | Cell Biology Developmental Biology Microscopy Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723001454 |
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