The Characterization and Differential Analysis of m<sup>6</sup>A Methylation in Hycole Rabbit Muscle and Adipose Tissue and Prediction of Regulatory Mechanism about Intramuscular Fat
N6-methyladenosine (m<sup>6</sup>A) widely participates in various life processes of animals, including disease, memory, growth and development, etc. However, there is no report on m<sup>6</sup>A regulating intramuscular fat deposition in rabbits. In this study, m<sup>6...
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MDPI AG
2023-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/3/446 |
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| author | Gang Luo Yaotian Ai Lin Yu Shuhui Wang Zhanjun Ren |
| author_facet | Gang Luo Yaotian Ai Lin Yu Shuhui Wang Zhanjun Ren |
| author_sort | Gang Luo |
| collection | DOAJ |
| description | N6-methyladenosine (m<sup>6</sup>A) widely participates in various life processes of animals, including disease, memory, growth and development, etc. However, there is no report on m<sup>6</sup>A regulating intramuscular fat deposition in rabbits. In this study, m<sup>6</sup>A modification of Hycole rabbit muscle and adipose tissues were detected by MeRIP-Seq. In this case, 3 methylases and 12 genes modified by m<sup>6</sup>A were found to be significantly different between muscle and adipose tissues. At the same time, we found 3 methylases can regulate the expression of 12 genes in different ways and the function of 12 genes is related to fat deposition base on existing studies. 12 genes were modified by m<sup>6</sup>A methylase in rabbit muscle and adipose tissues. These results suggest that 3 methylases may regulate the expression of 12 genes through different pathways. In addition, the analysis of results showed that 6 of the 12 genes regulated eight signaling pathways, which regulated intramuscular fat deposition. RT-qPCR was used to validate the sequencing results and found the expression results of RT-qPCR and sequencing results are consistent. In summary, <i>METTL4</i>, <i>ZC3H13</i> and <i>IGF2BP2</i> regulated intramuscular fat by m<sup>6</sup>A modified gene/signaling pathways. Our work provided a new molecular basis and a new way to produce rabbit meat with good taste. |
| first_indexed | 2024-03-11T09:54:31Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T09:54:31Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ef3d73451f7e4441a3ae88800ce587cd2023-11-16T16:01:00ZengMDPI AGAnimals2076-26152023-01-0113344610.3390/ani13030446The Characterization and Differential Analysis of m<sup>6</sup>A Methylation in Hycole Rabbit Muscle and Adipose Tissue and Prediction of Regulatory Mechanism about Intramuscular FatGang Luo0Yaotian Ai1Lin Yu2Shuhui Wang3Zhanjun Ren4College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaN6-methyladenosine (m<sup>6</sup>A) widely participates in various life processes of animals, including disease, memory, growth and development, etc. However, there is no report on m<sup>6</sup>A regulating intramuscular fat deposition in rabbits. In this study, m<sup>6</sup>A modification of Hycole rabbit muscle and adipose tissues were detected by MeRIP-Seq. In this case, 3 methylases and 12 genes modified by m<sup>6</sup>A were found to be significantly different between muscle and adipose tissues. At the same time, we found 3 methylases can regulate the expression of 12 genes in different ways and the function of 12 genes is related to fat deposition base on existing studies. 12 genes were modified by m<sup>6</sup>A methylase in rabbit muscle and adipose tissues. These results suggest that 3 methylases may regulate the expression of 12 genes through different pathways. In addition, the analysis of results showed that 6 of the 12 genes regulated eight signaling pathways, which regulated intramuscular fat deposition. RT-qPCR was used to validate the sequencing results and found the expression results of RT-qPCR and sequencing results are consistent. In summary, <i>METTL4</i>, <i>ZC3H13</i> and <i>IGF2BP2</i> regulated intramuscular fat by m<sup>6</sup>A modified gene/signaling pathways. Our work provided a new molecular basis and a new way to produce rabbit meat with good taste.https://www.mdpi.com/2076-2615/13/3/446Hycole rabbitm<sup>6</sup>Agene expressionmethylaseintramuscula fat |
| spellingShingle | Gang Luo Yaotian Ai Lin Yu Shuhui Wang Zhanjun Ren The Characterization and Differential Analysis of m<sup>6</sup>A Methylation in Hycole Rabbit Muscle and Adipose Tissue and Prediction of Regulatory Mechanism about Intramuscular Fat Animals Hycole rabbit m<sup>6</sup>A gene expression methylase intramuscula fat |
| title | The Characterization and Differential Analysis of m<sup>6</sup>A Methylation in Hycole Rabbit Muscle and Adipose Tissue and Prediction of Regulatory Mechanism about Intramuscular Fat |
| title_full | The Characterization and Differential Analysis of m<sup>6</sup>A Methylation in Hycole Rabbit Muscle and Adipose Tissue and Prediction of Regulatory Mechanism about Intramuscular Fat |
| title_fullStr | The Characterization and Differential Analysis of m<sup>6</sup>A Methylation in Hycole Rabbit Muscle and Adipose Tissue and Prediction of Regulatory Mechanism about Intramuscular Fat |
| title_full_unstemmed | The Characterization and Differential Analysis of m<sup>6</sup>A Methylation in Hycole Rabbit Muscle and Adipose Tissue and Prediction of Regulatory Mechanism about Intramuscular Fat |
| title_short | The Characterization and Differential Analysis of m<sup>6</sup>A Methylation in Hycole Rabbit Muscle and Adipose Tissue and Prediction of Regulatory Mechanism about Intramuscular Fat |
| title_sort | characterization and differential analysis of m sup 6 sup a methylation in hycole rabbit muscle and adipose tissue and prediction of regulatory mechanism about intramuscular fat |
| topic | Hycole rabbit m<sup>6</sup>A gene expression methylase intramuscula fat |
| url | https://www.mdpi.com/2076-2615/13/3/446 |
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