Biofertilizers regulate the soil microbial community and enhance Panax ginseng yields
Abstract Background Panax ginseng is widely used as functional food and traditional Chinese medicine. To satisfy the market supply and medication safety, biofertilizers are used as agents to stimulate the growth and production of P. ginseng. Methods In this study, we used high-throughput sequencing...
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Chinese Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s13020-019-0241-1 |
| _version_ | 1818896710526566400 |
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| author | Linlin Dong Yong Li Jiang Xu Juan Yang Guangfei Wei Liang Shen Wanlong Ding Shilin Chen |
| author_facet | Linlin Dong Yong Li Jiang Xu Juan Yang Guangfei Wei Liang Shen Wanlong Ding Shilin Chen |
| author_sort | Linlin Dong |
| collection | DOAJ |
| description | Abstract Background Panax ginseng is widely used as functional food and traditional Chinese medicine. To satisfy the market supply and medication safety, biofertilizers are used as agents to stimulate the growth and production of P. ginseng. Methods In this study, we used high-throughput sequencing and quantitative polymerase chain reaction to analyze microbial community in soils treated with biofertilizers during the development stages of P. ginseng. Ginsenoside content was detected using high-performance liquid chromatography analysis to evaluate the effects of biofertilizer application. Results In this study, the incidence rate of P. ginseng root rot significantly declined by 40.3–47.3% after application of disease-biocontrol biofertilizers. Bacterial diversity showed increasing trends in soils treated with 3.0–4.5 ml kg−1 of disease-biocontrol biofertilizers compared with those in untreated soils. Principal coordinate analysis ordination revealed that bacterial communities were changed by biofertilizers depending on their application concentration. Relative abundance of potentially beneficial bacterial agents, such as Bacillus, Burkholderia, Rhizobium, Streptomyces, and Mycobacterium, significantly increased compared with that in control. Fusarium of low abundance observed in soils treated with biofertilizers compared with that in untreated soils. P. ginseng yield was enhanced by 17.0–19.1%, and ginsenoside (Rg1 and Rb1) contents were improved after biofertilizer application. Conclusions Our results reveal that biofertilizers reduced the incidence rate of root rot, increased bacterial diversity, promoted the relative abundance of potentially beneficial bacterial taxa, decreased the abundance of potentially harmful bacterial agents, and then enhanced the yield and quality of P. ginseng. |
| first_indexed | 2024-12-19T19:04:37Z |
| format | Article |
| id | doaj.art-ef41da172f734630ba4dd13d8d6b755b |
| institution | Directory Open Access Journal |
| issn | 1749-8546 |
| language | English |
| last_indexed | 2024-12-19T19:04:37Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Chinese Medicine |
| spelling | doaj.art-ef41da172f734630ba4dd13d8d6b755b2022-12-21T20:09:28ZengBMCChinese Medicine1749-85462019-05-0114111410.1186/s13020-019-0241-1Biofertilizers regulate the soil microbial community and enhance Panax ginseng yieldsLinlin Dong0Yong Li1Jiang Xu2Juan Yang3Guangfei Wei4Liang Shen5Wanlong Ding6Shilin Chen7Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical CollegeKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical CollegeKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesAbstract Background Panax ginseng is widely used as functional food and traditional Chinese medicine. To satisfy the market supply and medication safety, biofertilizers are used as agents to stimulate the growth and production of P. ginseng. Methods In this study, we used high-throughput sequencing and quantitative polymerase chain reaction to analyze microbial community in soils treated with biofertilizers during the development stages of P. ginseng. Ginsenoside content was detected using high-performance liquid chromatography analysis to evaluate the effects of biofertilizer application. Results In this study, the incidence rate of P. ginseng root rot significantly declined by 40.3–47.3% after application of disease-biocontrol biofertilizers. Bacterial diversity showed increasing trends in soils treated with 3.0–4.5 ml kg−1 of disease-biocontrol biofertilizers compared with those in untreated soils. Principal coordinate analysis ordination revealed that bacterial communities were changed by biofertilizers depending on their application concentration. Relative abundance of potentially beneficial bacterial agents, such as Bacillus, Burkholderia, Rhizobium, Streptomyces, and Mycobacterium, significantly increased compared with that in control. Fusarium of low abundance observed in soils treated with biofertilizers compared with that in untreated soils. P. ginseng yield was enhanced by 17.0–19.1%, and ginsenoside (Rg1 and Rb1) contents were improved after biofertilizer application. Conclusions Our results reveal that biofertilizers reduced the incidence rate of root rot, increased bacterial diversity, promoted the relative abundance of potentially beneficial bacterial taxa, decreased the abundance of potentially harmful bacterial agents, and then enhanced the yield and quality of P. ginseng.http://link.springer.com/article/10.1186/s13020-019-0241-1Panax ginsengBiofertilizerSoil microbial communityRoot-rotHigh-throughput sequencing |
| spellingShingle | Linlin Dong Yong Li Jiang Xu Juan Yang Guangfei Wei Liang Shen Wanlong Ding Shilin Chen Biofertilizers regulate the soil microbial community and enhance Panax ginseng yields Chinese Medicine Panax ginseng Biofertilizer Soil microbial community Root-rot High-throughput sequencing |
| title | Biofertilizers regulate the soil microbial community and enhance Panax ginseng yields |
| title_full | Biofertilizers regulate the soil microbial community and enhance Panax ginseng yields |
| title_fullStr | Biofertilizers regulate the soil microbial community and enhance Panax ginseng yields |
| title_full_unstemmed | Biofertilizers regulate the soil microbial community and enhance Panax ginseng yields |
| title_short | Biofertilizers regulate the soil microbial community and enhance Panax ginseng yields |
| title_sort | biofertilizers regulate the soil microbial community and enhance panax ginseng yields |
| topic | Panax ginseng Biofertilizer Soil microbial community Root-rot High-throughput sequencing |
| url | http://link.springer.com/article/10.1186/s13020-019-0241-1 |
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