Soybean Antigen Protein-Induced Intestinal Barrier Damage by Trigging Endoplasmic Reticulum Stress and Disordering Gut Microbiota in Weaned Piglets
Endoplasmic reticulum (ER) stress is a crucial factor in the pathogenesis of intestinal diseases. Soybean antigenic proteins (<i>β</i>-conglycinin and soy glycinin) induce hypersensitivity reactions and intestinal barrier damage. However, whether this damage is associated with ER stress,...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/18/6500 |
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| author | Lei Wang Yujia Liu Daoliang Zhang Hongyan Ding Shibin Feng Chang Zhao Jinjie Wu Xichun Wang |
| author_facet | Lei Wang Yujia Liu Daoliang Zhang Hongyan Ding Shibin Feng Chang Zhao Jinjie Wu Xichun Wang |
| author_sort | Lei Wang |
| collection | DOAJ |
| description | Endoplasmic reticulum (ER) stress is a crucial factor in the pathogenesis of intestinal diseases. Soybean antigenic proteins (<i>β</i>-conglycinin and soy glycinin) induce hypersensitivity reactions and intestinal barrier damage. However, whether this damage is associated with ER stress, autophagy, and the gut microbiome is largely unclear. Therefore, in this study, we aimed to investigate the effect of dietary supplementation with soy glycinin (11S glycinin) and <i>β</i>-conglycinin (7S glycinin) on intestinal ER stress, autophagy, and flora in weaned piglets. Thirty healthy 21-day-old weaned “Duroc × Long White × Yorkshire” piglets were randomly divided into three groups and fed a basic, 7S-supplemented, or 11S-supplemented diet for one week. The results indicated that 7S/11S glycinin disrupted growth performance, damaged intestinal barrier integrity, and impaired goblet cell function in piglets (<i>p</i> < 0.05). Moreover, 7S/11S glycinin induced ER stress and blocked autophagic flux in the jejunum (<i>p</i> < 0.05) and increased the relative abundance of pathogenic flora (<i>p</i> < 0.01) and decreased that of beneficial flora (<i>p</i> < 0.05). In conclusion, 7S/11S glycinin induces intestinal ER stress, autophagic flux blockage, microbiota imbalance, and intestinal barrier damage in piglets. |
| first_indexed | 2024-03-10T22:25:38Z |
| format | Article |
| id | doaj.art-ef4958b6025049128bb1f99b44f4859d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T22:25:38Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ef4958b6025049128bb1f99b44f4859d2023-11-19T12:08:14ZengMDPI AGMolecules1420-30492023-09-012818650010.3390/molecules28186500Soybean Antigen Protein-Induced Intestinal Barrier Damage by Trigging Endoplasmic Reticulum Stress and Disordering Gut Microbiota in Weaned PigletsLei Wang0Yujia Liu1Daoliang Zhang2Hongyan Ding3Shibin Feng4Chang Zhao5Jinjie Wu6Xichun Wang7College of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, ChinaAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety Engineering, Institute of Animal Husbandry and Veterinary Medicine, Medicine Academy of Agricultural Sciences, Hefei 230061, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, ChinaEndoplasmic reticulum (ER) stress is a crucial factor in the pathogenesis of intestinal diseases. Soybean antigenic proteins (<i>β</i>-conglycinin and soy glycinin) induce hypersensitivity reactions and intestinal barrier damage. However, whether this damage is associated with ER stress, autophagy, and the gut microbiome is largely unclear. Therefore, in this study, we aimed to investigate the effect of dietary supplementation with soy glycinin (11S glycinin) and <i>β</i>-conglycinin (7S glycinin) on intestinal ER stress, autophagy, and flora in weaned piglets. Thirty healthy 21-day-old weaned “Duroc × Long White × Yorkshire” piglets were randomly divided into three groups and fed a basic, 7S-supplemented, or 11S-supplemented diet for one week. The results indicated that 7S/11S glycinin disrupted growth performance, damaged intestinal barrier integrity, and impaired goblet cell function in piglets (<i>p</i> < 0.05). Moreover, 7S/11S glycinin induced ER stress and blocked autophagic flux in the jejunum (<i>p</i> < 0.05) and increased the relative abundance of pathogenic flora (<i>p</i> < 0.01) and decreased that of beneficial flora (<i>p</i> < 0.05). In conclusion, 7S/11S glycinin induces intestinal ER stress, autophagic flux blockage, microbiota imbalance, and intestinal barrier damage in piglets.https://www.mdpi.com/1420-3049/28/18/6500endoplasmic reticulum stresssoybean glycinin<i>β</i>-conglycininautophagygut microbiota |
| spellingShingle | Lei Wang Yujia Liu Daoliang Zhang Hongyan Ding Shibin Feng Chang Zhao Jinjie Wu Xichun Wang Soybean Antigen Protein-Induced Intestinal Barrier Damage by Trigging Endoplasmic Reticulum Stress and Disordering Gut Microbiota in Weaned Piglets Molecules endoplasmic reticulum stress soybean glycinin <i>β</i>-conglycinin autophagy gut microbiota |
| title | Soybean Antigen Protein-Induced Intestinal Barrier Damage by Trigging Endoplasmic Reticulum Stress and Disordering Gut Microbiota in Weaned Piglets |
| title_full | Soybean Antigen Protein-Induced Intestinal Barrier Damage by Trigging Endoplasmic Reticulum Stress and Disordering Gut Microbiota in Weaned Piglets |
| title_fullStr | Soybean Antigen Protein-Induced Intestinal Barrier Damage by Trigging Endoplasmic Reticulum Stress and Disordering Gut Microbiota in Weaned Piglets |
| title_full_unstemmed | Soybean Antigen Protein-Induced Intestinal Barrier Damage by Trigging Endoplasmic Reticulum Stress and Disordering Gut Microbiota in Weaned Piglets |
| title_short | Soybean Antigen Protein-Induced Intestinal Barrier Damage by Trigging Endoplasmic Reticulum Stress and Disordering Gut Microbiota in Weaned Piglets |
| title_sort | soybean antigen protein induced intestinal barrier damage by trigging endoplasmic reticulum stress and disordering gut microbiota in weaned piglets |
| topic | endoplasmic reticulum stress soybean glycinin <i>β</i>-conglycinin autophagy gut microbiota |
| url | https://www.mdpi.com/1420-3049/28/18/6500 |
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