Phase Retrieval for Digital Holographic Microscopy With Defocused Holograms

A new phase retrieval technique in digital holographic microscopy (DHM) with three defocused holograms is proposed. Given the defocusing distance, the phase distributions of tested specimen can be reconstructed with a simple algebraic equation. We have deduced this equation in detail. To avoid the m...

Full description

Bibliographic Details
Main Authors: Dingnan Deng, Weijuan Qu, Wenqi He, Yu Wu, Xiaoli Liu, Xiang Peng
Format: Article
Language:English
Published: IEEE 2018-01-01
Series:IEEE Photonics Journal
Subjects:
Online Access:https://ieeexplore.ieee.org/document/8187637/
_version_ 1818369979322466304
author Dingnan Deng
Weijuan Qu
Wenqi He
Yu Wu
Xiaoli Liu
Xiang Peng
author_facet Dingnan Deng
Weijuan Qu
Wenqi He
Yu Wu
Xiaoli Liu
Xiang Peng
author_sort Dingnan Deng
collection DOAJ
description A new phase retrieval technique in digital holographic microscopy (DHM) with three defocused holograms is proposed. Given the defocusing distance, the phase distributions of tested specimen can be reconstructed with a simple algebraic equation. We have deduced this equation in detail. To avoid the manual operation, the defocused holograms can be flexibly and precisely obtained by introducing an electronically tunable lens based 4f system. This method is suitable for an on-axis hologram as well as the off-axis one but avoids the requirements for not only the iterative process, complex spectrum selection in off-axis DHM or additional phase-shifting devices in on-axis DHM but also the assumption of tested specimen or previous knowledge of the system. A series of simulations and the experimental results of the microlens array and water drop demonstrate the feasibility and effectiveness of the proposed method.
first_indexed 2024-12-13T23:32:26Z
format Article
id doaj.art-ef4edc47e6874bb1adf359f32f45c80d
institution Directory Open Access Journal
issn 1943-0655
language English
last_indexed 2024-12-13T23:32:26Z
publishDate 2018-01-01
publisher IEEE
record_format Article
series IEEE Photonics Journal
spelling doaj.art-ef4edc47e6874bb1adf359f32f45c80d2022-12-21T23:27:23ZengIEEEIEEE Photonics Journal1943-06552018-01-011011910.1109/JPHOT.2017.27826748187637Phase Retrieval for Digital Holographic Microscopy With Defocused HologramsDingnan Deng0https://orcid.org/0000-0003-4528-1064Weijuan Qu1Wenqi He2Yu Wu3Xiaoli Liu4Xiang Peng5College of Optoelectronics Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Guangdong Province, Shenzhen University, Shenzhen, ChinaNgee Ann Polytechnic, SingaporeCollege of Optoelectronics Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Guangdong Province, Shenzhen University, Shenzhen, ChinaCollege of Optoelectronics Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Guangdong Province, Shenzhen University, Shenzhen, ChinaCollege of Optoelectronics Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Guangdong Province, Shenzhen University, Shenzhen, ChinaCollege of Optoelectronics Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Guangdong Province, Shenzhen University, Shenzhen, ChinaA new phase retrieval technique in digital holographic microscopy (DHM) with three defocused holograms is proposed. Given the defocusing distance, the phase distributions of tested specimen can be reconstructed with a simple algebraic equation. We have deduced this equation in detail. To avoid the manual operation, the defocused holograms can be flexibly and precisely obtained by introducing an electronically tunable lens based 4f system. This method is suitable for an on-axis hologram as well as the off-axis one but avoids the requirements for not only the iterative process, complex spectrum selection in off-axis DHM or additional phase-shifting devices in on-axis DHM but also the assumption of tested specimen or previous knowledge of the system. A series of simulations and the experimental results of the microlens array and water drop demonstrate the feasibility and effectiveness of the proposed method.https://ieeexplore.ieee.org/document/8187637/Holographyphase measurementmicroscopy
spellingShingle Dingnan Deng
Weijuan Qu
Wenqi He
Yu Wu
Xiaoli Liu
Xiang Peng
Phase Retrieval for Digital Holographic Microscopy With Defocused Holograms
IEEE Photonics Journal
Holography
phase measurement
microscopy
title Phase Retrieval for Digital Holographic Microscopy With Defocused Holograms
title_full Phase Retrieval for Digital Holographic Microscopy With Defocused Holograms
title_fullStr Phase Retrieval for Digital Holographic Microscopy With Defocused Holograms
title_full_unstemmed Phase Retrieval for Digital Holographic Microscopy With Defocused Holograms
title_short Phase Retrieval for Digital Holographic Microscopy With Defocused Holograms
title_sort phase retrieval for digital holographic microscopy with defocused holograms
topic Holography
phase measurement
microscopy
url https://ieeexplore.ieee.org/document/8187637/
work_keys_str_mv AT dingnandeng phaseretrievalfordigitalholographicmicroscopywithdefocusedholograms
AT weijuanqu phaseretrievalfordigitalholographicmicroscopywithdefocusedholograms
AT wenqihe phaseretrievalfordigitalholographicmicroscopywithdefocusedholograms
AT yuwu phaseretrievalfordigitalholographicmicroscopywithdefocusedholograms
AT xiaoliliu phaseretrievalfordigitalholographicmicroscopywithdefocusedholograms
AT xiangpeng phaseretrievalfordigitalholographicmicroscopywithdefocusedholograms