A sustainable approach for the reliable and simultaneous determination of terpenoids and cannabinoids in hemp inflorescences by vacuum assisted headspace solid-phase microextraction
Cannabis sativa L. is an intriguing plant that has been exploited since ancient times for recreational, medical, textile and food purposes. The plant's most promising bioactive constituents discovered so far belong to the terpenoid and cannabinoid classes. These specialised metabolites are high...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Advances in Sample Preparation |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772582022000110 |
| _version_ | 1818524015846752256 |
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| author | Francesca Capetti Patrizia Rubiolo Giulia Mastellone Arianna Marengo Barbara Sgorbini Cecilia Cagliero |
| author_facet | Francesca Capetti Patrizia Rubiolo Giulia Mastellone Arianna Marengo Barbara Sgorbini Cecilia Cagliero |
| author_sort | Francesca Capetti |
| collection | DOAJ |
| description | Cannabis sativa L. is an intriguing plant that has been exploited since ancient times for recreational, medical, textile and food purposes. The plant's most promising bioactive constituents discovered so far belong to the terpenoid and cannabinoid classes. These specialised metabolites are highly concentrated in the plant aerial parts and their chemical characterisation is crucial to guarantee the safe and efficient use of the plant material irrespective of which use it is. This study investigates for the first time the use of vacuum assisted HS-SPME as a sample preparation process in an analytical protocol based on HS-SPME combined to fast GC-MS analysis that aims at comprehensively characterising both the terpenoid and cannabinoid profiles of Cannabis inflorescences in a single step. The results proved that vacuum in the HS should be preferred over atmospheric pressure conditions as it ensures the fast recovery of cannabinoid markers at relatively lower sampling temperatures (i.e., 90°C) that do not discriminate the most volatile fraction nor cause the formation of artefacts when the sampling time is minimised. |
| first_indexed | 2024-12-11T05:51:58Z |
| format | Article |
| id | doaj.art-ef51c5f3df3344d6afb49bd3874a4163 |
| institution | Directory Open Access Journal |
| issn | 2772-5820 |
| language | English |
| last_indexed | 2024-12-11T05:51:58Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Sample Preparation |
| spelling | doaj.art-ef51c5f3df3344d6afb49bd3874a41632022-12-22T01:18:48ZengElsevierAdvances in Sample Preparation2772-58202022-03-012100014A sustainable approach for the reliable and simultaneous determination of terpenoids and cannabinoids in hemp inflorescences by vacuum assisted headspace solid-phase microextractionFrancesca Capetti0Patrizia Rubiolo1Giulia Mastellone2Arianna Marengo3Barbara Sgorbini4Cecilia Cagliero5Dipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via P. Giuria 9, Turin 10125, ItalyDipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via P. Giuria 9, Turin 10125, ItalyDipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via P. Giuria 9, Turin 10125, ItalyDipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via P. Giuria 9, Turin 10125, ItalyDipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via P. Giuria 9, Turin 10125, ItalyCorresponding author.; Dipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via P. Giuria 9, Turin 10125, ItalyCannabis sativa L. is an intriguing plant that has been exploited since ancient times for recreational, medical, textile and food purposes. The plant's most promising bioactive constituents discovered so far belong to the terpenoid and cannabinoid classes. These specialised metabolites are highly concentrated in the plant aerial parts and their chemical characterisation is crucial to guarantee the safe and efficient use of the plant material irrespective of which use it is. This study investigates for the first time the use of vacuum assisted HS-SPME as a sample preparation process in an analytical protocol based on HS-SPME combined to fast GC-MS analysis that aims at comprehensively characterising both the terpenoid and cannabinoid profiles of Cannabis inflorescences in a single step. The results proved that vacuum in the HS should be preferred over atmospheric pressure conditions as it ensures the fast recovery of cannabinoid markers at relatively lower sampling temperatures (i.e., 90°C) that do not discriminate the most volatile fraction nor cause the formation of artefacts when the sampling time is minimised.http://www.sciencedirect.com/science/article/pii/S2772582022000110Cannabis sativa inflorescencesVacuum assisted headspace solid-phase microextractionVolatilomeTerpenoidsCannabinoids |
| spellingShingle | Francesca Capetti Patrizia Rubiolo Giulia Mastellone Arianna Marengo Barbara Sgorbini Cecilia Cagliero A sustainable approach for the reliable and simultaneous determination of terpenoids and cannabinoids in hemp inflorescences by vacuum assisted headspace solid-phase microextraction Advances in Sample Preparation Cannabis sativa inflorescences Vacuum assisted headspace solid-phase microextraction Volatilome Terpenoids Cannabinoids |
| title | A sustainable approach for the reliable and simultaneous determination of terpenoids and cannabinoids in hemp inflorescences by vacuum assisted headspace solid-phase microextraction |
| title_full | A sustainable approach for the reliable and simultaneous determination of terpenoids and cannabinoids in hemp inflorescences by vacuum assisted headspace solid-phase microextraction |
| title_fullStr | A sustainable approach for the reliable and simultaneous determination of terpenoids and cannabinoids in hemp inflorescences by vacuum assisted headspace solid-phase microextraction |
| title_full_unstemmed | A sustainable approach for the reliable and simultaneous determination of terpenoids and cannabinoids in hemp inflorescences by vacuum assisted headspace solid-phase microextraction |
| title_short | A sustainable approach for the reliable and simultaneous determination of terpenoids and cannabinoids in hemp inflorescences by vacuum assisted headspace solid-phase microextraction |
| title_sort | sustainable approach for the reliable and simultaneous determination of terpenoids and cannabinoids in hemp inflorescences by vacuum assisted headspace solid phase microextraction |
| topic | Cannabis sativa inflorescences Vacuum assisted headspace solid-phase microextraction Volatilome Terpenoids Cannabinoids |
| url | http://www.sciencedirect.com/science/article/pii/S2772582022000110 |
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