Dual loss of human POLQ and LIG4 abolishes random integration
Homologous recombination mediated gene targeting is highly inefficient in human cells due to random integration events, Here the authors show that dual repression of polymerase θ and DNA ligase IV eliminate random integration events.
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms16112 |
| _version_ | 1818345392610213888 |
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| author | Shinta Saito Ryo Maeda Noritaka Adachi |
| author_facet | Shinta Saito Ryo Maeda Noritaka Adachi |
| author_sort | Shinta Saito |
| collection | DOAJ |
| description | Homologous recombination mediated gene targeting is highly inefficient in human cells due to random integration events, Here the authors show that dual repression of polymerase θ and DNA ligase IV eliminate random integration events. |
| first_indexed | 2024-12-13T17:01:39Z |
| format | Article |
| id | doaj.art-ef54135e9bea42c6a525eac4bbb609dc |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-13T17:01:39Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-ef54135e9bea42c6a525eac4bbb609dc2022-12-21T23:37:48ZengNature PortfolioNature Communications2041-17232017-07-018111010.1038/ncomms16112Dual loss of human POLQ and LIG4 abolishes random integrationShinta Saito0Ryo Maeda1Noritaka Adachi2Department of Life and Environmental System Science, Graduate School of Nanobioscience, Yokohama City UniversityDepartment of Biology, Graduate School of Science, Chiba UniversityDepartment of Life and Environmental System Science, Graduate School of Nanobioscience, Yokohama City UniversityHomologous recombination mediated gene targeting is highly inefficient in human cells due to random integration events, Here the authors show that dual repression of polymerase θ and DNA ligase IV eliminate random integration events.https://doi.org/10.1038/ncomms16112 |
| spellingShingle | Shinta Saito Ryo Maeda Noritaka Adachi Dual loss of human POLQ and LIG4 abolishes random integration Nature Communications |
| title | Dual loss of human POLQ and LIG4 abolishes random integration |
| title_full | Dual loss of human POLQ and LIG4 abolishes random integration |
| title_fullStr | Dual loss of human POLQ and LIG4 abolishes random integration |
| title_full_unstemmed | Dual loss of human POLQ and LIG4 abolishes random integration |
| title_short | Dual loss of human POLQ and LIG4 abolishes random integration |
| title_sort | dual loss of human polq and lig4 abolishes random integration |
| url | https://doi.org/10.1038/ncomms16112 |
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