Apoptosis Cell Death Effect of Scrophularia Variegata on Breast Cancer Cells via Mitochondrial Intrinsic Pathway
Purpose: Scrophularia variegata M. Beib. (Scrophulariaceae) is an Iranian medicinal plant which is used for various inflammatory disorders in traditional medicine. In this study we evaluated the anti-cancer and cytotoxic effects of the Scrophularia variegata (S. variegata) ethanolic extract on the h...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2015-09-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-5-443.pdf |
| _version_ | 1819088484618469376 |
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| author | Abbas Azadmehr Reza Hajiaghaee Behzad Baradaran Hashem Haghdoost-Yazdi |
| author_facet | Abbas Azadmehr Reza Hajiaghaee Behzad Baradaran Hashem Haghdoost-Yazdi |
| author_sort | Abbas Azadmehr |
| collection | DOAJ |
| description | Purpose: Scrophularia variegata M. Beib. (Scrophulariaceae) is an Iranian medicinal
plant which is used for various inflammatory disorders in traditional medicine.
In this study we evaluated the anti-cancer and cytotoxic effects of the Scrophularia variegata (S. variegata) ethanolic extract on the human breast cancer cell line.
Methods: The cytotoxicity effect of the extract on MCF-7 cells
was evaluated by MTT assay. In addition, Caspase activity, DNA ladder and Cell
death were evaluated by ELISA, gel electrophoresis and Annexin V-FITC/PI
staining, respectively.
Results: The S. variegata extract showed significant effect cytotoxicity on MCF-7
human breast cancer cell line. Treatment with the extract induced
apoptosis on the breast cancer cells by cell cycle arrest in G2/M phase. The
results indicated that cytotoxicity activity was associated with an increase of
apoptosis as demonstrated by DNA fragmentation as well as an increase of the
amount of caspase 3 and caspase 9. In addition, the phytochemical assay showed
that the extract had antioxidant capacity and also flavonoids, phenolic
compounds and phenyl propanoids were presented in the extract.
Conclusion:
Our findings indicated that S. variegata extract
induced apoptosis via mitochondrial intrinsic pathway on breast cancer by cell
cycle arrest in G2/M phase and an increase of caspase 3 and caspase 9. However
future studies are needed. |
| first_indexed | 2024-12-21T21:52:47Z |
| format | Article |
| id | doaj.art-ef5771fa128a43c7b070e585681baa3f |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-12-21T21:52:47Z |
| publishDate | 2015-09-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-ef5771fa128a43c7b070e585681baa3f2022-12-21T18:49:03ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082015-09-015344344610.15171/apb.2015.060APB_2693_20150125145129Apoptosis Cell Death Effect of Scrophularia Variegata on Breast Cancer Cells via Mitochondrial Intrinsic PathwayAbbas Azadmehr0Reza Hajiaghaee1Behzad Baradaran2Hashem Haghdoost-Yazdi3 Immunology Department, Qazvin University of Medical Sciences, Qazvin, Iran. Pharmacognosy and Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Physiology Department, Qazvin University of Medical Sciences, Qazvin, Iran.Purpose: Scrophularia variegata M. Beib. (Scrophulariaceae) is an Iranian medicinal plant which is used for various inflammatory disorders in traditional medicine. In this study we evaluated the anti-cancer and cytotoxic effects of the Scrophularia variegata (S. variegata) ethanolic extract on the human breast cancer cell line. Methods: The cytotoxicity effect of the extract on MCF-7 cells was evaluated by MTT assay. In addition, Caspase activity, DNA ladder and Cell death were evaluated by ELISA, gel electrophoresis and Annexin V-FITC/PI staining, respectively. Results: The S. variegata extract showed significant effect cytotoxicity on MCF-7 human breast cancer cell line. Treatment with the extract induced apoptosis on the breast cancer cells by cell cycle arrest in G2/M phase. The results indicated that cytotoxicity activity was associated with an increase of apoptosis as demonstrated by DNA fragmentation as well as an increase of the amount of caspase 3 and caspase 9. In addition, the phytochemical assay showed that the extract had antioxidant capacity and also flavonoids, phenolic compounds and phenyl propanoids were presented in the extract. Conclusion: Our findings indicated that S. variegata extract induced apoptosis via mitochondrial intrinsic pathway on breast cancer by cell cycle arrest in G2/M phase and an increase of caspase 3 and caspase 9. However future studies are needed.http://journals.tbzmed.ac.ir/APB/Manuscript/APB-5-443.pdfScrophularia variegataApoptosisBreast cancer cell lineCaspase |
| spellingShingle | Abbas Azadmehr Reza Hajiaghaee Behzad Baradaran Hashem Haghdoost-Yazdi Apoptosis Cell Death Effect of Scrophularia Variegata on Breast Cancer Cells via Mitochondrial Intrinsic Pathway Advanced Pharmaceutical Bulletin Scrophularia variegata Apoptosis Breast cancer cell line Caspase |
| title | Apoptosis
Cell Death Effect of Scrophularia Variegata on Breast Cancer Cells via
Mitochondrial Intrinsic Pathway |
| title_full | Apoptosis
Cell Death Effect of Scrophularia Variegata on Breast Cancer Cells via
Mitochondrial Intrinsic Pathway |
| title_fullStr | Apoptosis
Cell Death Effect of Scrophularia Variegata on Breast Cancer Cells via
Mitochondrial Intrinsic Pathway |
| title_full_unstemmed | Apoptosis
Cell Death Effect of Scrophularia Variegata on Breast Cancer Cells via
Mitochondrial Intrinsic Pathway |
| title_short | Apoptosis
Cell Death Effect of Scrophularia Variegata on Breast Cancer Cells via
Mitochondrial Intrinsic Pathway |
| title_sort | apoptosis cell death effect of scrophularia variegata on breast cancer cells via mitochondrial intrinsic pathway |
| topic | Scrophularia variegata Apoptosis Breast cancer cell line Caspase |
| url | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-5-443.pdf |
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