Increasing Student Understanding of Microscope Optics by Building and Testing the Limits of Simple, Hand-Made Model Microscopes

The ability to effectively use a microscope to observe microorganisms is a crucial skill required for many disciplines within biology, especially general microbiology and cell biology. A basic understanding of the optical properties of light microscopes is required for students to use microscopes ef...

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Main Authors: Kevin Drace, Brett Couch, Patrick J. Keeling
Format: Article
Language:English
Published: American Society for Microbiology 2012-01-01
Series:Journal of Microbiology & Biology Education
Online Access:https://journals.asm.org/doi/10.1128/jmbe.v13i1.374
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author Kevin Drace
Brett Couch
Patrick J. Keeling
author_facet Kevin Drace
Brett Couch
Patrick J. Keeling
author_sort Kevin Drace
collection DOAJ
description The ability to effectively use a microscope to observe microorganisms is a crucial skill required for many disciplines within biology, especially general microbiology and cell biology. A basic understanding of the optical properties of light microscopes is required for students to use microscopes effectively, but this subject can also be a challenge to make personally interesting to students. To explore basic optical principles of magnification and resolving power in a more engaging and hands-on fashion, students constructed handmade lenses and microscopes based on Antony van Leeuwenhoek’s design using simple materials—paper, staples, glass, and adhesive putty. Students determined the power of their lenses using a green laser pointer to magnify a copper grid of known size, which also allowed students to examine variables affecting the power and resolution of a lens such as diameter, working distance, and wavelength of light. To assess the effectiveness of the laboratory’s learning objectives, four sections of a general microbiology course were given a brief pre-activity assessment quiz to determine their background knowledge on the subject. One week after the laboratory activity, students were given the same quiz (unannounced) under similar conditions. Students showed significant gains in their understanding of microscope optics.
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spelling doaj.art-ef580c74e408494394322930a1fe97192022-12-21T19:20:16ZengAmerican Society for MicrobiologyJournal of Microbiology & Biology Education1935-78771935-78852012-01-01131454910.1128/jmbe.v13i1.374Increasing Student Understanding of Microscope Optics by Building and Testing the Limits of Simple, Hand-Made Model MicroscopesKevin Drace0Brett Couch1Patrick J. Keeling2Department of Biology, Mercer University, Macon, GA 31207Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T1Z4Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T1Z4The ability to effectively use a microscope to observe microorganisms is a crucial skill required for many disciplines within biology, especially general microbiology and cell biology. A basic understanding of the optical properties of light microscopes is required for students to use microscopes effectively, but this subject can also be a challenge to make personally interesting to students. To explore basic optical principles of magnification and resolving power in a more engaging and hands-on fashion, students constructed handmade lenses and microscopes based on Antony van Leeuwenhoek’s design using simple materials—paper, staples, glass, and adhesive putty. Students determined the power of their lenses using a green laser pointer to magnify a copper grid of known size, which also allowed students to examine variables affecting the power and resolution of a lens such as diameter, working distance, and wavelength of light. To assess the effectiveness of the laboratory’s learning objectives, four sections of a general microbiology course were given a brief pre-activity assessment quiz to determine their background knowledge on the subject. One week after the laboratory activity, students were given the same quiz (unannounced) under similar conditions. Students showed significant gains in their understanding of microscope optics.https://journals.asm.org/doi/10.1128/jmbe.v13i1.374
spellingShingle Kevin Drace
Brett Couch
Patrick J. Keeling
Increasing Student Understanding of Microscope Optics by Building and Testing the Limits of Simple, Hand-Made Model Microscopes
Journal of Microbiology & Biology Education
title Increasing Student Understanding of Microscope Optics by Building and Testing the Limits of Simple, Hand-Made Model Microscopes
title_full Increasing Student Understanding of Microscope Optics by Building and Testing the Limits of Simple, Hand-Made Model Microscopes
title_fullStr Increasing Student Understanding of Microscope Optics by Building and Testing the Limits of Simple, Hand-Made Model Microscopes
title_full_unstemmed Increasing Student Understanding of Microscope Optics by Building and Testing the Limits of Simple, Hand-Made Model Microscopes
title_short Increasing Student Understanding of Microscope Optics by Building and Testing the Limits of Simple, Hand-Made Model Microscopes
title_sort increasing student understanding of microscope optics by building and testing the limits of simple hand made model microscopes
url https://journals.asm.org/doi/10.1128/jmbe.v13i1.374
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