Soluble CD163 correlates with lipid metabolic adaptations in type 1 diabetes patients during ketoacidosis
Abstract Introduction Diabetic ketoacidosis (DKA) is associated with inflammation and increased lipolysis. The macrophage activation marker, soluble CD163 (sCD163), is associated with obesity, non‐alcoholic fatty liver disease and type 2 diabetes. We aimed to investigate whether sCD163 correlates wi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Diabetes Investigation |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jdi.12869 |
| _version_ | 1818609047267442688 |
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| author | Mads Svart Nikolaj Rittig Niels Møller Holger J Møller Henning Gronbaek |
| author_facet | Mads Svart Nikolaj Rittig Niels Møller Holger J Møller Henning Gronbaek |
| author_sort | Mads Svart |
| collection | DOAJ |
| description | Abstract Introduction Diabetic ketoacidosis (DKA) is associated with inflammation and increased lipolysis. The macrophage activation marker, soluble CD163 (sCD163), is associated with obesity, non‐alcoholic fatty liver disease and type 2 diabetes. We aimed to investigate whether sCD163 correlates with key elements of lipolysis in type 1 diabetes patients during mild DKA. Materials and Methods We investigated nine patients with type 1 diabetes twice during: (i) euglycemic control conditions and a bolus of saline; and (ii) hyperglycemic ketotic conditions induced by lipopolysaccharide administration combined with insulin deprivation. Blood samples, indirect calorimetry, palmitate tracer and adipose tissue biopsies were used to investigate lipid metabolism. Results We observed a significant increase in plasma sCD163 levels after lipopolysaccharide exposure (P < 0.001). Concentrations of sCD163 were positively correlated with plasma concentrations of free fatty acids, palmitate rate of appearance and lipid oxidation rates, and negatively correlated to the expression of G0/G1 switch 2 gene messenger ribonucleic acid content in adipose tissue (P < 0.01 for all). Furthermore, sCD163 levels correlated positively with plasma peak concentrations of cortisol, glucagon, tumor necrosis factor‐α, interleukin‐6 and interleukin‐10 (P < 0.01 for all). Data on lipolysis and inflammation have previously been published. Conclusions Macrophage activation assessed by sCD163 might play an important role in DKA, as it correlates strongly with important components of lipid metabolism including free fatty acids, palmitate, lipid oxidation, G0/G1 switch 2 gene and pro‐inflammatory cytokines during initial steps of DKA. These results are novel and add important knowledge to the field of DKA. |
| first_indexed | 2024-12-16T14:52:19Z |
| format | Article |
| id | doaj.art-ef58201c45e5488d814c2189148f91b2 |
| institution | Directory Open Access Journal |
| issn | 2040-1116 2040-1124 |
| language | English |
| last_indexed | 2024-12-16T14:52:19Z |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Investigation |
| spelling | doaj.art-ef58201c45e5488d814c2189148f91b22022-12-21T22:27:34ZengWileyJournal of Diabetes Investigation2040-11162040-11242019-01-01101677210.1111/jdi.12869Soluble CD163 correlates with lipid metabolic adaptations in type 1 diabetes patients during ketoacidosisMads Svart0Nikolaj Rittig1Niels Møller2Holger J Møller3Henning Gronbaek4Department of Internal Medicine and Endocrinology (MEA) and Medical Research Laboratory Aarhus University Hospital Aarhus C DenmarkDepartment of Internal Medicine and Endocrinology (MEA) and Medical Research Laboratory Aarhus University Hospital Aarhus C DenmarkDepartment of Internal Medicine and Endocrinology (MEA) and Medical Research Laboratory Aarhus University Hospital Aarhus C DenmarkDepartment of Clinical Biochemistry Aarhus University Hospital Aarhus NDenmarkDepartment of Hepatology and Gastroenterology Aarhus University Aarhus C DenmarkAbstract Introduction Diabetic ketoacidosis (DKA) is associated with inflammation and increased lipolysis. The macrophage activation marker, soluble CD163 (sCD163), is associated with obesity, non‐alcoholic fatty liver disease and type 2 diabetes. We aimed to investigate whether sCD163 correlates with key elements of lipolysis in type 1 diabetes patients during mild DKA. Materials and Methods We investigated nine patients with type 1 diabetes twice during: (i) euglycemic control conditions and a bolus of saline; and (ii) hyperglycemic ketotic conditions induced by lipopolysaccharide administration combined with insulin deprivation. Blood samples, indirect calorimetry, palmitate tracer and adipose tissue biopsies were used to investigate lipid metabolism. Results We observed a significant increase in plasma sCD163 levels after lipopolysaccharide exposure (P < 0.001). Concentrations of sCD163 were positively correlated with plasma concentrations of free fatty acids, palmitate rate of appearance and lipid oxidation rates, and negatively correlated to the expression of G0/G1 switch 2 gene messenger ribonucleic acid content in adipose tissue (P < 0.01 for all). Furthermore, sCD163 levels correlated positively with plasma peak concentrations of cortisol, glucagon, tumor necrosis factor‐α, interleukin‐6 and interleukin‐10 (P < 0.01 for all). Data on lipolysis and inflammation have previously been published. Conclusions Macrophage activation assessed by sCD163 might play an important role in DKA, as it correlates strongly with important components of lipid metabolism including free fatty acids, palmitate, lipid oxidation, G0/G1 switch 2 gene and pro‐inflammatory cytokines during initial steps of DKA. These results are novel and add important knowledge to the field of DKA.https://doi.org/10.1111/jdi.12869G0/G1 switch 2 geneKetoacidosisSoluble CD163 |
| spellingShingle | Mads Svart Nikolaj Rittig Niels Møller Holger J Møller Henning Gronbaek Soluble CD163 correlates with lipid metabolic adaptations in type 1 diabetes patients during ketoacidosis Journal of Diabetes Investigation G0/G1 switch 2 gene Ketoacidosis Soluble CD163 |
| title | Soluble CD163 correlates with lipid metabolic adaptations in type 1 diabetes patients during ketoacidosis |
| title_full | Soluble CD163 correlates with lipid metabolic adaptations in type 1 diabetes patients during ketoacidosis |
| title_fullStr | Soluble CD163 correlates with lipid metabolic adaptations in type 1 diabetes patients during ketoacidosis |
| title_full_unstemmed | Soluble CD163 correlates with lipid metabolic adaptations in type 1 diabetes patients during ketoacidosis |
| title_short | Soluble CD163 correlates with lipid metabolic adaptations in type 1 diabetes patients during ketoacidosis |
| title_sort | soluble cd163 correlates with lipid metabolic adaptations in type 1 diabetes patients during ketoacidosis |
| topic | G0/G1 switch 2 gene Ketoacidosis Soluble CD163 |
| url | https://doi.org/10.1111/jdi.12869 |
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