Improving fatty acid availability for bio-hydrocarbon production in Escherichia coli by metabolic engineering.
Previous studies have demonstrated the feasibility of producing fatty-acid-derived hydrocarbons in Escherichia coli. However, product titers and yields remain low. In this work, we demonstrate new methods for improving fatty acid production by modifying central carbon metabolism and storing fatty ac...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3798384?pdf=render |
| _version_ | 1818503471076212736 |
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| author | Fengming Lin Yu Chen Robert Levine Kilho Lee Yingjin Yuan Xiaoxia Nina Lin |
| author_facet | Fengming Lin Yu Chen Robert Levine Kilho Lee Yingjin Yuan Xiaoxia Nina Lin |
| author_sort | Fengming Lin |
| collection | DOAJ |
| description | Previous studies have demonstrated the feasibility of producing fatty-acid-derived hydrocarbons in Escherichia coli. However, product titers and yields remain low. In this work, we demonstrate new methods for improving fatty acid production by modifying central carbon metabolism and storing fatty acids in triacylglycerol. Based on suggestions from a computational model, we deleted seven genes involved in aerobic respiration, mixed-acid fermentation, and glyoxylate bypass (in the order of cyoA, nuoA, ndh, adhE, dld, pta, and iclR) to modify the central carbon metabolic/regulatory networks. These gene deletions led to increased total fatty acids, which were the highest in the mutants containing five or six gene knockouts. Additionally, when two key enzymes in the fatty acid biosynthesis pathway were over-expressed, we observed further increase in strain △cyoA△adhE△nuoA△ndh△pta△dld, leading to 202 mg/g dry cell weight of total fatty acids, ~250% of that in the wild-type strain. Meanwhile, we successfully introduced a triacylglycerol biosynthesis pathway into E. coli through heterologous expression of wax ester synthase/acyl-coenzyme:diacylglycerol acyltransferase (WS/DGAT) enzymes. The added pathway improved both the amount and fuel quality of the fatty acids. These new metabolic engineering strategies are providing promising directions for future investigation. |
| first_indexed | 2024-12-10T21:24:26Z |
| format | Article |
| id | doaj.art-ef5841c656cb4474b325938c2fbd27f4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T21:24:26Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ef5841c656cb4474b325938c2fbd27f42022-12-22T01:33:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7859510.1371/journal.pone.0078595Improving fatty acid availability for bio-hydrocarbon production in Escherichia coli by metabolic engineering.Fengming LinYu ChenRobert LevineKilho LeeYingjin YuanXiaoxia Nina LinPrevious studies have demonstrated the feasibility of producing fatty-acid-derived hydrocarbons in Escherichia coli. However, product titers and yields remain low. In this work, we demonstrate new methods for improving fatty acid production by modifying central carbon metabolism and storing fatty acids in triacylglycerol. Based on suggestions from a computational model, we deleted seven genes involved in aerobic respiration, mixed-acid fermentation, and glyoxylate bypass (in the order of cyoA, nuoA, ndh, adhE, dld, pta, and iclR) to modify the central carbon metabolic/regulatory networks. These gene deletions led to increased total fatty acids, which were the highest in the mutants containing five or six gene knockouts. Additionally, when two key enzymes in the fatty acid biosynthesis pathway were over-expressed, we observed further increase in strain △cyoA△adhE△nuoA△ndh△pta△dld, leading to 202 mg/g dry cell weight of total fatty acids, ~250% of that in the wild-type strain. Meanwhile, we successfully introduced a triacylglycerol biosynthesis pathway into E. coli through heterologous expression of wax ester synthase/acyl-coenzyme:diacylglycerol acyltransferase (WS/DGAT) enzymes. The added pathway improved both the amount and fuel quality of the fatty acids. These new metabolic engineering strategies are providing promising directions for future investigation.http://europepmc.org/articles/PMC3798384?pdf=render |
| spellingShingle | Fengming Lin Yu Chen Robert Levine Kilho Lee Yingjin Yuan Xiaoxia Nina Lin Improving fatty acid availability for bio-hydrocarbon production in Escherichia coli by metabolic engineering. PLoS ONE |
| title | Improving fatty acid availability for bio-hydrocarbon production in Escherichia coli by metabolic engineering. |
| title_full | Improving fatty acid availability for bio-hydrocarbon production in Escherichia coli by metabolic engineering. |
| title_fullStr | Improving fatty acid availability for bio-hydrocarbon production in Escherichia coli by metabolic engineering. |
| title_full_unstemmed | Improving fatty acid availability for bio-hydrocarbon production in Escherichia coli by metabolic engineering. |
| title_short | Improving fatty acid availability for bio-hydrocarbon production in Escherichia coli by metabolic engineering. |
| title_sort | improving fatty acid availability for bio hydrocarbon production in escherichia coli by metabolic engineering |
| url | http://europepmc.org/articles/PMC3798384?pdf=render |
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