Fine-needle aspiration for diagnosis of tuberculous lymphadenitis in children in Bangui, Central African Republic
<p>Abstract</p> <p>Background</p> <p>Tuberculosis (TB) is a major cause of childhood morbidity and mortality in developing countries. One of the main difficulties is obtaining adequate specimens for bacteriological confirmation of the disease in children.</p> <...
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| Format: | Article |
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BMC
2012-12-01
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| Series: | BMC Pediatrics |
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| Online Access: | http://www.biomedcentral.com/1471-2431/12/191 |
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| author | Fanny Minime-Lingoupou Beyam Narcisse Gody Jean Chrusostome Zandanga G Yango F Manirakiza Alexandre Rigouts Leen Pierre-Audigier Catherine Gicquel Brigitte Bobossi Gustave |
| author_facet | Fanny Minime-Lingoupou Beyam Narcisse Gody Jean Chrusostome Zandanga G Yango F Manirakiza Alexandre Rigouts Leen Pierre-Audigier Catherine Gicquel Brigitte Bobossi Gustave |
| author_sort | Fanny Minime-Lingoupou |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Tuberculosis (TB) is a major cause of childhood morbidity and mortality in developing countries. One of the main difficulties is obtaining adequate specimens for bacteriological confirmation of the disease in children.</p> <p>The aim of this study is to evaluate the adequacy of fine-needle aspiration (FNA) for the diagnosis of TB.</p> <p>Methods</p> <p>In a prospective study conducted at the paediatric hospital in Bangui in 2007–2009, we used fine-needle aspiration to obtain samples for diagnosis of TB from 131 children aged 0–17 years with persistent lymphadenitis.</p> <p>Results</p> <p>Fine-needle aspiration provided samples that could be used for bacteriological confirmation of TB. Ziehl-Neelsen staining for acid-fast bacilli was positive in 42.7% of samples, and culture identified TB in 67.2% of cases. Of 75 samples that were stain-negative, 49 (65.3%) were culture-positive, while 12 stain-positive samples remained culture-negative. Ten of the 12 stain-positive, culture-negative samples were from patients who had received previous antimicrobial therapy. With regard to phenotypic drug susceptibility, 81/88 strains (91.1%) were fully susceptible to isoniazid, rifampicin, ethambutol and streptomycin, six (6.8%) were resistant to one drug, and one multidrug-resistant strain was found.</p> <p>Conclusions</p> <p>Fine-needle aspiration is simple, cost-effective and non-invasive and can be performed by trained staff. Combined with rapid molecular diagnostic tests, fine-needle aspirates could improve the diagnosis of TB and provide valuable information for appropriate treatment and drug resistance.</p> |
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| institution | Directory Open Access Journal |
| issn | 1471-2431 |
| language | English |
| last_indexed | 2024-12-17T15:17:32Z |
| publishDate | 2012-12-01 |
| publisher | BMC |
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| series | BMC Pediatrics |
| spelling | doaj.art-ef5b2f73b7904ac3bdaa5a2886f981de2022-12-21T21:43:29ZengBMCBMC Pediatrics1471-24312012-12-0112119110.1186/1471-2431-12-191Fine-needle aspiration for diagnosis of tuberculous lymphadenitis in children in Bangui, Central African RepublicFanny Minime-LingoupouBeyam NarcisseGody Jean ChrusostomeZandanga GYango FManirakiza AlexandreRigouts LeenPierre-Audigier CatherineGicquel BrigitteBobossi Gustave<p>Abstract</p> <p>Background</p> <p>Tuberculosis (TB) is a major cause of childhood morbidity and mortality in developing countries. One of the main difficulties is obtaining adequate specimens for bacteriological confirmation of the disease in children.</p> <p>The aim of this study is to evaluate the adequacy of fine-needle aspiration (FNA) for the diagnosis of TB.</p> <p>Methods</p> <p>In a prospective study conducted at the paediatric hospital in Bangui in 2007–2009, we used fine-needle aspiration to obtain samples for diagnosis of TB from 131 children aged 0–17 years with persistent lymphadenitis.</p> <p>Results</p> <p>Fine-needle aspiration provided samples that could be used for bacteriological confirmation of TB. Ziehl-Neelsen staining for acid-fast bacilli was positive in 42.7% of samples, and culture identified TB in 67.2% of cases. Of 75 samples that were stain-negative, 49 (65.3%) were culture-positive, while 12 stain-positive samples remained culture-negative. Ten of the 12 stain-positive, culture-negative samples were from patients who had received previous antimicrobial therapy. With regard to phenotypic drug susceptibility, 81/88 strains (91.1%) were fully susceptible to isoniazid, rifampicin, ethambutol and streptomycin, six (6.8%) were resistant to one drug, and one multidrug-resistant strain was found.</p> <p>Conclusions</p> <p>Fine-needle aspiration is simple, cost-effective and non-invasive and can be performed by trained staff. Combined with rapid molecular diagnostic tests, fine-needle aspirates could improve the diagnosis of TB and provide valuable information for appropriate treatment and drug resistance.</p>http://www.biomedcentral.com/1471-2431/12/191TuberculosisLymphadenitisFine needle aspirationDiagnosis |
| spellingShingle | Fanny Minime-Lingoupou Beyam Narcisse Gody Jean Chrusostome Zandanga G Yango F Manirakiza Alexandre Rigouts Leen Pierre-Audigier Catherine Gicquel Brigitte Bobossi Gustave Fine-needle aspiration for diagnosis of tuberculous lymphadenitis in children in Bangui, Central African Republic BMC Pediatrics Tuberculosis Lymphadenitis Fine needle aspiration Diagnosis |
| title | Fine-needle aspiration for diagnosis of tuberculous lymphadenitis in children in Bangui, Central African Republic |
| title_full | Fine-needle aspiration for diagnosis of tuberculous lymphadenitis in children in Bangui, Central African Republic |
| title_fullStr | Fine-needle aspiration for diagnosis of tuberculous lymphadenitis in children in Bangui, Central African Republic |
| title_full_unstemmed | Fine-needle aspiration for diagnosis of tuberculous lymphadenitis in children in Bangui, Central African Republic |
| title_short | Fine-needle aspiration for diagnosis of tuberculous lymphadenitis in children in Bangui, Central African Republic |
| title_sort | fine needle aspiration for diagnosis of tuberculous lymphadenitis in children in bangui central african republic |
| topic | Tuberculosis Lymphadenitis Fine needle aspiration Diagnosis |
| url | http://www.biomedcentral.com/1471-2431/12/191 |
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