Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil
Two polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2001-08-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822001000400015&tlng=en |
| _version_ | 1818553872899112960 |
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| author | Vânia Maria Sabadoto Brienze Ângela Pedroso Tonon Fabrício José Tarelho Pereira Elisabete Liso Waldir Antônio Tognola Manoel Armando Azevedo dos Santos Marcelo Urbano Ferreira |
| author_facet | Vânia Maria Sabadoto Brienze Ângela Pedroso Tonon Fabrício José Tarelho Pereira Elisabete Liso Waldir Antônio Tognola Manoel Armando Azevedo dos Santos Marcelo Urbano Ferreira |
| author_sort | Vânia Maria Sabadoto Brienze |
| collection | DOAJ |
| description | Two polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in 91 patients in southeastern Brazil. Only three PCR-positive, microbiologically negative patients were found. Analysis of sequential cerebrospinal fluid samples by nested PCR detected Mycobacterium tuberculosis DNA up to 29 days after the introduction of antituberculosis chemotherapy. |
| first_indexed | 2024-12-12T09:31:30Z |
| format | Article |
| id | doaj.art-ef5b991d200f4459b4de754e93c52414 |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-12-12T09:31:30Z |
| publishDate | 2001-08-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-ef5b991d200f4459b4de754e93c524142022-12-22T00:28:51ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492001-08-01344389393Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern BrazilVânia Maria Sabadoto Brienze0Ângela Pedroso Tonon1Fabrício José Tarelho Pereira2Elisabete Liso3Waldir Antônio Tognola4Manoel Armando Azevedo dos Santos5Marcelo Urbano Ferreira6Faculdade de Medicina de São José do Rio PretoFaculdade de Medicina de São José do Rio PretoFaculdade de Medicina de São José do Rio PretoFaculdade de Medicina de São José do Rio PretoFaculdade de Medicina de São José do Rio PretoUniversidade de São PauloUniversidade de São PauloTwo polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in 91 patients in southeastern Brazil. Only three PCR-positive, microbiologically negative patients were found. Analysis of sequential cerebrospinal fluid samples by nested PCR detected Mycobacterium tuberculosis DNA up to 29 days after the introduction of antituberculosis chemotherapy.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822001000400015&tlng=enDiagnosis of tuberculous meningitisPCRMycobacterium tuberculosis |
| spellingShingle | Vânia Maria Sabadoto Brienze Ângela Pedroso Tonon Fabrício José Tarelho Pereira Elisabete Liso Waldir Antônio Tognola Manoel Armando Azevedo dos Santos Marcelo Urbano Ferreira Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil Revista da Sociedade Brasileira de Medicina Tropical Diagnosis of tuberculous meningitis PCR Mycobacterium tuberculosis |
| title | Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil |
| title_full | Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil |
| title_fullStr | Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil |
| title_full_unstemmed | Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil |
| title_short | Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil |
| title_sort | low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern brazil |
| topic | Diagnosis of tuberculous meningitis PCR Mycobacterium tuberculosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822001000400015&tlng=en |
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