Application of CRISPR-Cas System to Mitigate Superbug Infections
Multidrug resistance in bacterial strains known as superbugs is estimated to cause fatal infections worldwide. Migration and urbanization have resulted in overcrowding and inadequate sanitation, contributing to a high risk of superbug infections within and between different communities. The CRISPR-C...
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MDPI AG
2023-09-01
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author | Ali A. Rabaan Mona A. Al Fares Manar Almaghaslah Tariq Alpakistany Nawal A. Al Kaabi Saleh A. Alshamrani Ahmad A. Alshehri Ibrahim Abdullah Almazni Ahmed Saif Abdulrahim R. Hakami Faryal Khamis Mubarak Alfaresi Zainab Alsalem Zainab A. Alsoliabi Kawthar Amur Salim Al Amri Amal K. Hassoueh Ranjan K. Mohapatra Kovy Arteaga-Livias Mohammed Alissa |
author_facet | Ali A. Rabaan Mona A. Al Fares Manar Almaghaslah Tariq Alpakistany Nawal A. Al Kaabi Saleh A. Alshamrani Ahmad A. Alshehri Ibrahim Abdullah Almazni Ahmed Saif Abdulrahim R. Hakami Faryal Khamis Mubarak Alfaresi Zainab Alsalem Zainab A. Alsoliabi Kawthar Amur Salim Al Amri Amal K. Hassoueh Ranjan K. Mohapatra Kovy Arteaga-Livias Mohammed Alissa |
author_sort | Ali A. Rabaan |
collection | DOAJ |
description | Multidrug resistance in bacterial strains known as superbugs is estimated to cause fatal infections worldwide. Migration and urbanization have resulted in overcrowding and inadequate sanitation, contributing to a high risk of superbug infections within and between different communities. The CRISPR-Cas system, mainly type II, has been projected as a robust tool to precisely edit drug-resistant bacterial genomes to combat antibiotic-resistant bacterial strains effectively. To entirely opt for its potential, advanced development in the CRISPR-Cas system is needed to reduce toxicity and promote efficacy in gene-editing applications. This might involve base-editing techniques used to produce point mutations. These methods employ designed Cas9 variations, such as the adenine base editor (ABE) and the cytidine base editor (CBE), to directly edit single base pairs without causing DSBs. The CBE and ABE could change a target base pair into a different one (for example, G-C to A-T or C-G to A-T). In this review, we addressed the limitations of the CRISPR/Cas system and explored strategies for circumventing these limitations by applying diverse base-editing techniques. Furthermore, we also discussed recent research showcasing the ability of base editors to eliminate drug-resistant microbes. |
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language | English |
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publishDate | 2023-09-01 |
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series | Microorganisms |
spelling | doaj.art-ef5c5d5c7aed4f2db9e6455982cc918b2023-11-19T17:26:14ZengMDPI AGMicroorganisms2076-26072023-09-011110240410.3390/microorganisms11102404Application of CRISPR-Cas System to Mitigate Superbug InfectionsAli A. Rabaan0Mona A. Al Fares1Manar Almaghaslah2Tariq Alpakistany3Nawal A. Al Kaabi4Saleh A. Alshamrani5Ahmad A. Alshehri6Ibrahim Abdullah Almazni7Ahmed Saif8Abdulrahim R. Hakami9Faryal Khamis10Mubarak Alfaresi11Zainab Alsalem12Zainab A. Alsoliabi13Kawthar Amur Salim Al Amri14Amal K. Hassoueh15Ranjan K. Mohapatra16Kovy Arteaga-Livias17Mohammed Alissa18Molecular Diagnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi ArabiaDepartment of Internal Medicine, King Abdulaziz University Hospital, Jeddah 21589, Saudi ArabiaInfectious Disease Division, Department of Internal Medicine, Dammam Medical Complex, Dammam 32245, Saudi ArabiaBacteriology Department, Public Health Laboratory, Taif 26521, Saudi ArabiaCollege of Medicine and Health Science, Khalifa University, Abu Dhabi 127788, United Arab EmiratesDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Najran University, Najran 61441, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Najran University, Najran 61441, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Najran University, Najran 61441, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha 62223, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha 62223, Saudi ArabiaInfection Diseases Unit, Department of Internal Medicine, Royal Hospital, Muscat 1331, OmanDepartment of Pathology and Laboratory Medicine, Zayed Military Hospital, Abu Dhabi 3740, United Arab EmiratesDepartment of Epidemic Diseases Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi ArabiaPharmacy Department, Qatif Central Hospital, Qatif 32654, Saudi ArabiaInfection and Control Department, Armed Forces Hospital, Azaibah 130, OmanPharmacy Department, King Saud Medical City, Riyadh 7790, Saudi ArabiaDepartment of Chemistry, Government College of Engineering, Keonjhar 758002, IndiaEscuela de Medicina-Filial Ica, Universidad Privada San Juan Bautista, Ica 11000, PeruDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaMultidrug resistance in bacterial strains known as superbugs is estimated to cause fatal infections worldwide. Migration and urbanization have resulted in overcrowding and inadequate sanitation, contributing to a high risk of superbug infections within and between different communities. The CRISPR-Cas system, mainly type II, has been projected as a robust tool to precisely edit drug-resistant bacterial genomes to combat antibiotic-resistant bacterial strains effectively. To entirely opt for its potential, advanced development in the CRISPR-Cas system is needed to reduce toxicity and promote efficacy in gene-editing applications. This might involve base-editing techniques used to produce point mutations. These methods employ designed Cas9 variations, such as the adenine base editor (ABE) and the cytidine base editor (CBE), to directly edit single base pairs without causing DSBs. The CBE and ABE could change a target base pair into a different one (for example, G-C to A-T or C-G to A-T). In this review, we addressed the limitations of the CRISPR/Cas system and explored strategies for circumventing these limitations by applying diverse base-editing techniques. Furthermore, we also discussed recent research showcasing the ability of base editors to eliminate drug-resistant microbes.https://www.mdpi.com/2076-2607/11/10/2404superbugsCRISPR/Casbase editingCBEABEbase editors |
spellingShingle | Ali A. Rabaan Mona A. Al Fares Manar Almaghaslah Tariq Alpakistany Nawal A. Al Kaabi Saleh A. Alshamrani Ahmad A. Alshehri Ibrahim Abdullah Almazni Ahmed Saif Abdulrahim R. Hakami Faryal Khamis Mubarak Alfaresi Zainab Alsalem Zainab A. Alsoliabi Kawthar Amur Salim Al Amri Amal K. Hassoueh Ranjan K. Mohapatra Kovy Arteaga-Livias Mohammed Alissa Application of CRISPR-Cas System to Mitigate Superbug Infections Microorganisms superbugs CRISPR/Cas base editing CBE ABE base editors |
title | Application of CRISPR-Cas System to Mitigate Superbug Infections |
title_full | Application of CRISPR-Cas System to Mitigate Superbug Infections |
title_fullStr | Application of CRISPR-Cas System to Mitigate Superbug Infections |
title_full_unstemmed | Application of CRISPR-Cas System to Mitigate Superbug Infections |
title_short | Application of CRISPR-Cas System to Mitigate Superbug Infections |
title_sort | application of crispr cas system to mitigate superbug infections |
topic | superbugs CRISPR/Cas base editing CBE ABE base editors |
url | https://www.mdpi.com/2076-2607/11/10/2404 |
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