Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus
In the terrestrial ciliate Colpoda cucullus, an elevation of the intracellular cAMP concentration was reported to be involved in environment-resistant resting cyst formation. In the present study, cAMP-dependently phosphorylated proteins of encystment-induced C. cucullus were isolated with Phos-tag...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Modena and Reggio Emilia
2014-06-01
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| Series: | Invertebrate Survival Journal |
| Subjects: | |
| Online Access: | https://isj02.unimore.it/index.php/ISJ/article/view/323 |
| _version_ | 1818915838415077376 |
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| author | Y Sogame K Kojima T Takeshita E Kinoshita T Matsuoka |
| author_facet | Y Sogame K Kojima T Takeshita E Kinoshita T Matsuoka |
| author_sort | Y Sogame |
| collection | DOAJ |
| description | In the terrestrial ciliate Colpoda cucullus, an elevation of the intracellular cAMP concentration was reported to be involved in environment-resistant resting cyst formation. In the present study, cAMP-dependently phosphorylated proteins of encystment-induced C. cucullus were isolated with Phos-tag agarose phosphate-affinity beads and subsequent SDS-PAGE. In a liquid
chromatography/tandem mass spectrometry analysis of these phosphoproteins, 27-, 37- and 43-kDa proteins (p27, p37 and p43) were identified as Rieske iron-sulfur protein, histone H4 (hyperacetylated form), and actin, respectively. |
| first_indexed | 2024-12-20T00:08:38Z |
| format | Article |
| id | doaj.art-ef6460ae411947eb8b3ed137aa46b8b7 |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-20T00:08:38Z |
| publishDate | 2014-06-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-ef6460ae411947eb8b3ed137aa46b8b72022-12-21T20:00:34ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2014-06-01111Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullusY Sogame0K Kojima1T Takeshita2E Kinoshita3T Matsuoka4Department of Biological Science, Faculty of Science, Kochi University, Kochi 780-8520, JapanDepartment of Microbiology and Immunology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, JapanDepartment of Microbiology and Immunology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, JapanDepartment of Functional Molecular Science, Institute of Biomedical & Health Sciences, Hiroshima University, Kasumi 1-2-3, Hiroshima 734-8553, JapanDepartment of Biological Science, Faculty of Science, Kochi University, Kochi 780-8520, JapanIn the terrestrial ciliate Colpoda cucullus, an elevation of the intracellular cAMP concentration was reported to be involved in environment-resistant resting cyst formation. In the present study, cAMP-dependently phosphorylated proteins of encystment-induced C. cucullus were isolated with Phos-tag agarose phosphate-affinity beads and subsequent SDS-PAGE. In a liquid chromatography/tandem mass spectrometry analysis of these phosphoproteins, 27-, 37- and 43-kDa proteins (p27, p37 and p43) were identified as Rieske iron-sulfur protein, histone H4 (hyperacetylated form), and actin, respectively.https://isj02.unimore.it/index.php/ISJ/article/view/323environment-resistant cystColpodaencystmentprotein phosphorylationcAMP |
| spellingShingle | Y Sogame K Kojima T Takeshita E Kinoshita T Matsuoka Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus Invertebrate Survival Journal environment-resistant cyst Colpoda encystment protein phosphorylation cAMP |
| title | Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus |
| title_full | Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus |
| title_fullStr | Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus |
| title_full_unstemmed | Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus |
| title_short | Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus |
| title_sort | identification of camp dependent phosphorylated proteins involved in the formation of environment resistant resting cysts by the terrestrial ciliate colpoda cucullus |
| topic | environment-resistant cyst Colpoda encystment protein phosphorylation cAMP |
| url | https://isj02.unimore.it/index.php/ISJ/article/view/323 |
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