Molecular detection of Anaplasma infections in ixodid ticks from the Qinghai-Tibet Plateau
Abstract Anaplasma species are tick-transmitted obligate intracellular bacteria that infect many wild and domestic animals and humans. The prevalence of Anaplasma spp. in ixodid ticks of Qinghai Province is poorly understood. In this study, a total of 1104 questing adult ticks were investigated for...
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| Format: | Article |
| Language: | English |
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BMC
2019-02-01
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| Series: | Infectious Diseases of Poverty |
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| Online Access: | http://link.springer.com/article/10.1186/s40249-019-0522-z |
| _version_ | 1811263324977889280 |
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| author | Rong Han Ji-Fei Yang Muhammad Uzair Mukhtar Ze Chen Qing-Li Niu Yuan-Qing Lin Guang-Yuan Liu Jian-Xun Luo Hong Yin Zhi-Jie Liu |
| author_facet | Rong Han Ji-Fei Yang Muhammad Uzair Mukhtar Ze Chen Qing-Li Niu Yuan-Qing Lin Guang-Yuan Liu Jian-Xun Luo Hong Yin Zhi-Jie Liu |
| author_sort | Rong Han |
| collection | DOAJ |
| description | Abstract Anaplasma species are tick-transmitted obligate intracellular bacteria that infect many wild and domestic animals and humans. The prevalence of Anaplasma spp. in ixodid ticks of Qinghai Province is poorly understood. In this study, a total of 1104 questing adult ticks were investigated for the infection of Anaplasma species. As a result, we demonstrated the total infection rates of 3.1, 11.1, 5.6, and 4.5% for A. phagocytophilum, A. bovis, A. ovis and A. capra, respectively. All of the tick samples were negative for A. marginale. The positive rates of A. phagocytophilum, A. ovis and A. capra in different tick species were significantly different. The positive rates of A. capra and A. bovis in the male ticks were significantly higher than that in the female ticks. Sequence analysis of A. ovis showed 99.5–100% identity to the previous reported isolates. The sequences of A. phagocytophilum had 100% identity to strains Ap-SHX21, JC3–3 and ZAM dog-181 from sheep, Mongolian gazelles, and dogs. Two genotypes of A. capra were found based on 16S rRNA, citrate synthase (gltA) gene and heat shock protein (groEL) gene analysis. In conclusion, A. bovis, A. ovis, A. phagocytophilum, and A. capra were present in the ticks in Qinghai Province. Anaplasma infection is associated with tick species, gender and distribution. These data will be helpful for understanding prevalence status of Anaplasma infections in ticks in Qinghai-Tibet Plateau. |
| first_indexed | 2024-04-12T19:42:35Z |
| format | Article |
| id | doaj.art-ef6498d3902d49efae6076629192ca2c |
| institution | Directory Open Access Journal |
| issn | 2049-9957 |
| language | English |
| last_indexed | 2024-04-12T19:42:35Z |
| publishDate | 2019-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Infectious Diseases of Poverty |
| spelling | doaj.art-ef6498d3902d49efae6076629192ca2c2022-12-22T03:19:03ZengBMCInfectious Diseases of Poverty2049-99572019-02-01811810.1186/s40249-019-0522-zMolecular detection of Anaplasma infections in ixodid ticks from the Qinghai-Tibet PlateauRong Han0Ji-Fei Yang1Muhammad Uzair Mukhtar2Ze Chen3Qing-Li Niu4Yuan-Qing Lin5Guang-Yuan Liu6Jian-Xun Luo7Hong Yin8Zhi-Jie Liu9State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesQinghai Provincial Center for Animal Disease Control and PreventionState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesAbstract Anaplasma species are tick-transmitted obligate intracellular bacteria that infect many wild and domestic animals and humans. The prevalence of Anaplasma spp. in ixodid ticks of Qinghai Province is poorly understood. In this study, a total of 1104 questing adult ticks were investigated for the infection of Anaplasma species. As a result, we demonstrated the total infection rates of 3.1, 11.1, 5.6, and 4.5% for A. phagocytophilum, A. bovis, A. ovis and A. capra, respectively. All of the tick samples were negative for A. marginale. The positive rates of A. phagocytophilum, A. ovis and A. capra in different tick species were significantly different. The positive rates of A. capra and A. bovis in the male ticks were significantly higher than that in the female ticks. Sequence analysis of A. ovis showed 99.5–100% identity to the previous reported isolates. The sequences of A. phagocytophilum had 100% identity to strains Ap-SHX21, JC3–3 and ZAM dog-181 from sheep, Mongolian gazelles, and dogs. Two genotypes of A. capra were found based on 16S rRNA, citrate synthase (gltA) gene and heat shock protein (groEL) gene analysis. In conclusion, A. bovis, A. ovis, A. phagocytophilum, and A. capra were present in the ticks in Qinghai Province. Anaplasma infection is associated with tick species, gender and distribution. These data will be helpful for understanding prevalence status of Anaplasma infections in ticks in Qinghai-Tibet Plateau.http://link.springer.com/article/10.1186/s40249-019-0522-zAnaplasmaTickSequence analysisPrevalence |
| spellingShingle | Rong Han Ji-Fei Yang Muhammad Uzair Mukhtar Ze Chen Qing-Li Niu Yuan-Qing Lin Guang-Yuan Liu Jian-Xun Luo Hong Yin Zhi-Jie Liu Molecular detection of Anaplasma infections in ixodid ticks from the Qinghai-Tibet Plateau Infectious Diseases of Poverty Anaplasma Tick Sequence analysis Prevalence |
| title | Molecular detection of Anaplasma infections in ixodid ticks from the Qinghai-Tibet Plateau |
| title_full | Molecular detection of Anaplasma infections in ixodid ticks from the Qinghai-Tibet Plateau |
| title_fullStr | Molecular detection of Anaplasma infections in ixodid ticks from the Qinghai-Tibet Plateau |
| title_full_unstemmed | Molecular detection of Anaplasma infections in ixodid ticks from the Qinghai-Tibet Plateau |
| title_short | Molecular detection of Anaplasma infections in ixodid ticks from the Qinghai-Tibet Plateau |
| title_sort | molecular detection of anaplasma infections in ixodid ticks from the qinghai tibet plateau |
| topic | Anaplasma Tick Sequence analysis Prevalence |
| url | http://link.springer.com/article/10.1186/s40249-019-0522-z |
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