Model class A and class L peptides increase the production of apoA-I-containing lipoproteins in HepG2 cells
Class A peptides inhibit atherosclerosis and protect cells from class L peptide-mediated lysis. Because the cytolytic process is concentration dependent, we hypothesized that at certain concentrations both classes of peptides exert similar effect(s) on cells. To test this hypothesis, we studied the...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2004-10-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520312669 |
| _version_ | 1819072379702214656 |
|---|---|
| author | Nassrin Dashti Geeta Datta Medha Manchekar Manjula Chaddha G.M. Anantharamaiah |
| author_facet | Nassrin Dashti Geeta Datta Medha Manchekar Manjula Chaddha G.M. Anantharamaiah |
| author_sort | Nassrin Dashti |
| collection | DOAJ |
| description | Class A peptides inhibit atherosclerosis and protect cells from class L peptide-mediated lysis. Because the cytolytic process is concentration dependent, we hypothesized that at certain concentrations both classes of peptides exert similar effect(s) on cells. To test this hypothesis, we studied the effects of a class L peptide (18L = GIKKFLGSIWKFIKAFVG) and a class A peptide, 18A-Pro-18A (18A = DWLKAFYDKVAEKLKEAF) (37pA), on apolipoprotein and lipoprotein production in HepG2 cells. Secretion of 35S-labeled apolipoprotein A-I (apoA-I) was stimulated by both 18L (110%) and 37pA (135%) at 10 and 20 nM of peptides, respectively. Both peptides enhanced the secretion of 3H-labeled phospholipids by 140% and 14C-labeled HDL-cholesterol (HDL-C) by 35% but had no significant effect on the total cholesterol mass or secretion.These results indicate that class L and class A peptides cause redistribution of cholesterol among lipoproteins in favor of HDL-C. Both peptides remodeled apoA-I-containing particles forming preβ- as well as α-HDL. This study suggests that increased secretion of phospholipids and apoA-I and the formation of preβ-HDL particles might contribute to the antiatherogenic properties of these peptides. |
| first_indexed | 2024-12-21T17:36:48Z |
| format | Article |
| id | doaj.art-ef6502b3689f43c98f95731f47733361 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T17:36:48Z |
| publishDate | 2004-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-ef6502b3689f43c98f95731f477333612022-12-21T18:55:45ZengElsevierJournal of Lipid Research0022-22752004-10-01451019191928Model class A and class L peptides increase the production of apoA-I-containing lipoproteins in HepG2 cellsNassrin Dashti0Geeta Datta1Medha Manchekar2Manjula Chaddha3G.M. Anantharamaiah4Department of Medicine, Biochemistry, and Molecular Genetics, and Atherosclerosis Research Unit, University of Alabama at Birmingham, Birmingham, AL 35294Department of Medicine, Biochemistry, and Molecular Genetics, and Atherosclerosis Research Unit, University of Alabama at Birmingham, Birmingham, AL 35294Department of Medicine, Biochemistry, and Molecular Genetics, and Atherosclerosis Research Unit, University of Alabama at Birmingham, Birmingham, AL 35294Department of Medicine, Biochemistry, and Molecular Genetics, and Atherosclerosis Research Unit, University of Alabama at Birmingham, Birmingham, AL 35294Department of Medicine, Biochemistry, and Molecular Genetics, and Atherosclerosis Research Unit, University of Alabama at Birmingham, Birmingham, AL 35294Class A peptides inhibit atherosclerosis and protect cells from class L peptide-mediated lysis. Because the cytolytic process is concentration dependent, we hypothesized that at certain concentrations both classes of peptides exert similar effect(s) on cells. To test this hypothesis, we studied the effects of a class L peptide (18L = GIKKFLGSIWKFIKAFVG) and a class A peptide, 18A-Pro-18A (18A = DWLKAFYDKVAEKLKEAF) (37pA), on apolipoprotein and lipoprotein production in HepG2 cells. Secretion of 35S-labeled apolipoprotein A-I (apoA-I) was stimulated by both 18L (110%) and 37pA (135%) at 10 and 20 nM of peptides, respectively. Both peptides enhanced the secretion of 3H-labeled phospholipids by 140% and 14C-labeled HDL-cholesterol (HDL-C) by 35% but had no significant effect on the total cholesterol mass or secretion.These results indicate that class L and class A peptides cause redistribution of cholesterol among lipoproteins in favor of HDL-C. Both peptides remodeled apoA-I-containing particles forming preβ- as well as α-HDL. This study suggests that increased secretion of phospholipids and apoA-I and the formation of preβ-HDL particles might contribute to the antiatherogenic properties of these peptides.http://www.sciencedirect.com/science/article/pii/S0022227520312669apolipoprotein A-Iapolipoprotein Bhigh density lipoprotein metabolismlow density lipoprotein metabolismtriglyceride secretioncholesterol secretion |
| spellingShingle | Nassrin Dashti Geeta Datta Medha Manchekar Manjula Chaddha G.M. Anantharamaiah Model class A and class L peptides increase the production of apoA-I-containing lipoproteins in HepG2 cells Journal of Lipid Research apolipoprotein A-I apolipoprotein B high density lipoprotein metabolism low density lipoprotein metabolism triglyceride secretion cholesterol secretion |
| title | Model class A and class L peptides increase the production of apoA-I-containing lipoproteins in HepG2 cells |
| title_full | Model class A and class L peptides increase the production of apoA-I-containing lipoproteins in HepG2 cells |
| title_fullStr | Model class A and class L peptides increase the production of apoA-I-containing lipoproteins in HepG2 cells |
| title_full_unstemmed | Model class A and class L peptides increase the production of apoA-I-containing lipoproteins in HepG2 cells |
| title_short | Model class A and class L peptides increase the production of apoA-I-containing lipoproteins in HepG2 cells |
| title_sort | model class a and class l peptides increase the production of apoa i containing lipoproteins in hepg2 cells |
| topic | apolipoprotein A-I apolipoprotein B high density lipoprotein metabolism low density lipoprotein metabolism triglyceride secretion cholesterol secretion |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520312669 |
| work_keys_str_mv | AT nassrindashti modelclassaandclasslpeptidesincreasetheproductionofapoaicontaininglipoproteinsinhepg2cells AT geetadatta modelclassaandclasslpeptidesincreasetheproductionofapoaicontaininglipoproteinsinhepg2cells AT medhamanchekar modelclassaandclasslpeptidesincreasetheproductionofapoaicontaininglipoproteinsinhepg2cells AT manjulachaddha modelclassaandclasslpeptidesincreasetheproductionofapoaicontaininglipoproteinsinhepg2cells AT gmanantharamaiah modelclassaandclasslpeptidesincreasetheproductionofapoaicontaininglipoproteinsinhepg2cells |