Characterization and functional analysis of the Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) gene family in poplar
Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) divides the mass flux to H, G and S units in monolignol biosynthesis and affects lignin content. Ten HCT homologs were identified in the Populus trichocarpa (Torr. & Gray) genome. Both genome duplication and tandem duplication re...
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PeerJ Inc.
2021-02-01
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| Online Access: | https://peerj.com/articles/10741.pdf |
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| author | Nan Chao Qi Qi Shuang Li Brent Ruan Xiangning Jiang Ying Gai |
| author_facet | Nan Chao Qi Qi Shuang Li Brent Ruan Xiangning Jiang Ying Gai |
| author_sort | Nan Chao |
| collection | DOAJ |
| description | Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) divides the mass flux to H, G and S units in monolignol biosynthesis and affects lignin content. Ten HCT homologs were identified in the Populus trichocarpa (Torr. & Gray) genome. Both genome duplication and tandem duplication resulted in the expansion of HCT orthologs in Populus. Comprehensive analysis including motif analysis, phylogenetic analysis, expression profiles and co-expression analysis revealed the divergence and putative function of these candidate PoptrHCTs. PoptrHCT1 and 2 were identified as likely involved in lignin biosynthesis. PoptrHCT9 and 10- are likely to be involved in plant development and the response to cold stress. Similar functional divergence was also identified in Populus tomentosa Carr. Enzymatic assay of PtoHCT1 showed that PtoHCT1 was able to synthesize caffeoyl shikimate using caffeoyl-CoA and shikimic acid as substrates. |
| first_indexed | 2024-03-09T08:02:04Z |
| format | Article |
| id | doaj.art-ef6522a54578478d914a03bfe6dfa2b0 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:02:04Z |
| publishDate | 2021-02-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-ef6522a54578478d914a03bfe6dfa2b02023-12-03T00:41:45ZengPeerJ Inc.PeerJ2167-83592021-02-019e1074110.7717/peerj.10741Characterization and functional analysis of the Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) gene family in poplarNan Chao0Qi Qi1Shuang Li2Brent Ruan3Xiangning Jiang4Ying Gai5School of Life Science, Tsinghua University, Beijing, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaDepartment of Agricultural and Biological Engineering, University of Illinois at Urbana Champaign, Urbana Champaign, IL, USACollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaHydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) divides the mass flux to H, G and S units in monolignol biosynthesis and affects lignin content. Ten HCT homologs were identified in the Populus trichocarpa (Torr. & Gray) genome. Both genome duplication and tandem duplication resulted in the expansion of HCT orthologs in Populus. Comprehensive analysis including motif analysis, phylogenetic analysis, expression profiles and co-expression analysis revealed the divergence and putative function of these candidate PoptrHCTs. PoptrHCT1 and 2 were identified as likely involved in lignin biosynthesis. PoptrHCT9 and 10- are likely to be involved in plant development and the response to cold stress. Similar functional divergence was also identified in Populus tomentosa Carr. Enzymatic assay of PtoHCT1 showed that PtoHCT1 was able to synthesize caffeoyl shikimate using caffeoyl-CoA and shikimic acid as substrates.https://peerj.com/articles/10741.pdfHydroxycinnamoyl- CoA: shikimate hydroxycinnamoyl transferaseEnzymatic synthesisDivergenceGene familyMonolignolPopulus |
| spellingShingle | Nan Chao Qi Qi Shuang Li Brent Ruan Xiangning Jiang Ying Gai Characterization and functional analysis of the Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) gene family in poplar PeerJ Hydroxycinnamoyl- CoA: shikimate hydroxycinnamoyl transferase Enzymatic synthesis Divergence Gene family Monolignol Populus |
| title | Characterization and functional analysis of the Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) gene family in poplar |
| title_full | Characterization and functional analysis of the Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) gene family in poplar |
| title_fullStr | Characterization and functional analysis of the Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) gene family in poplar |
| title_full_unstemmed | Characterization and functional analysis of the Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) gene family in poplar |
| title_short | Characterization and functional analysis of the Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) gene family in poplar |
| title_sort | characterization and functional analysis of the hydroxycinnamoyl coa shikimate hydroxycinnamoyl transferase hct gene family in poplar |
| topic | Hydroxycinnamoyl- CoA: shikimate hydroxycinnamoyl transferase Enzymatic synthesis Divergence Gene family Monolignol Populus |
| url | https://peerj.com/articles/10741.pdf |
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