Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus Detection
Lab-on-Chip (LoC) devices for performing real-time PCR are advantageous compared to standard equipment since these systems allow to conduct in-field quick analysis. The development of LoCs, where the components for performing the nucleic acid amplification are all integrated, can be an issue. In thi...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/5/544 |
| _version_ | 1827741871180349440 |
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| author | Francesca Costantini Nicola Lovecchio Manasa Nandimandalam Ariana Manglli Francesco Faggioli Mara Biasin Cesare Manetti Pio Federico Roversi Augusto Nascetti Giampiero de Cesare Domenico Caputo |
| author_facet | Francesca Costantini Nicola Lovecchio Manasa Nandimandalam Ariana Manglli Francesco Faggioli Mara Biasin Cesare Manetti Pio Federico Roversi Augusto Nascetti Giampiero de Cesare Domenico Caputo |
| author_sort | Francesca Costantini |
| collection | DOAJ |
| description | Lab-on-Chip (LoC) devices for performing real-time PCR are advantageous compared to standard equipment since these systems allow to conduct in-field quick analysis. The development of LoCs, where the components for performing the nucleic acid amplification are all integrated, can be an issue. In this work, we present a LoC-PCR device where thermalization, temperature control and detection elements are all integrated on a single glass substrate named System-on-Glass (SoG) obtained using metal thin-film deposition. By using a microwell plate optically coupled with the SoG, real-time reverse transcriptase PCR of RNA extracted from both a plant and human virus has been carried out in the developed LoC-PCR device. The limit of detection and time of analysis for the detection of the two viruses by using the LoC-PCR were compared with those achieved by standard equipment. The results showed that the two systems can detect the same concentration of RNA; however, the LoC-PCR performs the analysis in half of the time compared to the standard thermocycler, with the advantage of the portability, leading to a point-of-care device for several diagnostic applications. |
| first_indexed | 2024-03-11T03:54:17Z |
| format | Article |
| id | doaj.art-ef677ce84fdb41a9a52f9c60191ce37f |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T03:54:17Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-ef677ce84fdb41a9a52f9c60191ce37f2023-11-18T00:41:13ZengMDPI AGBiosensors2079-63742023-05-0113554410.3390/bios13050544Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus DetectionFrancesca Costantini0Nicola Lovecchio1Manasa Nandimandalam2Ariana Manglli3Francesco Faggioli4Mara Biasin5Cesare Manetti6Pio Federico Roversi7Augusto Nascetti8Giampiero de Cesare9Domenico Caputo10CREA Research Centre for Plant Protection and Certification, 00156 Rome, ItalyDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, ItalyCREA Research Centre for Plant Protection and Certification, 00156 Rome, ItalyCREA Research Centre for Plant Protection and Certification, 00156 Rome, ItalyDepartment of Biomedical and Clinical Sciences, University of Milan, Via G.B. Grassi, 20122 Milan, ItalyDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, ItalyCREA Research Centre for Plant Protection and Certification, 00156 Rome, ItalySchool of Aerospace Engineering, Sapienza University of Rome, 00138 Rome, ItalyDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, ItalyLab-on-Chip (LoC) devices for performing real-time PCR are advantageous compared to standard equipment since these systems allow to conduct in-field quick analysis. The development of LoCs, where the components for performing the nucleic acid amplification are all integrated, can be an issue. In this work, we present a LoC-PCR device where thermalization, temperature control and detection elements are all integrated on a single glass substrate named System-on-Glass (SoG) obtained using metal thin-film deposition. By using a microwell plate optically coupled with the SoG, real-time reverse transcriptase PCR of RNA extracted from both a plant and human virus has been carried out in the developed LoC-PCR device. The limit of detection and time of analysis for the detection of the two viruses by using the LoC-PCR were compared with those achieved by standard equipment. The results showed that the two systems can detect the same concentration of RNA; however, the LoC-PCR performs the analysis in half of the time compared to the standard thermocycler, with the advantage of the portability, leading to a point-of-care device for several diagnostic applications.https://www.mdpi.com/2079-6374/13/5/544Lab-on-Chipreal-time RT-PCRSystem-on-Glassvirus detection |
| spellingShingle | Francesca Costantini Nicola Lovecchio Manasa Nandimandalam Ariana Manglli Francesco Faggioli Mara Biasin Cesare Manetti Pio Federico Roversi Augusto Nascetti Giampiero de Cesare Domenico Caputo Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus Detection Biosensors Lab-on-Chip real-time RT-PCR System-on-Glass virus detection |
| title | Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus Detection |
| title_full | Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus Detection |
| title_fullStr | Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus Detection |
| title_full_unstemmed | Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus Detection |
| title_short | Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus Detection |
| title_sort | biomolecular monitoring tool based on lab on chip for virus detection |
| topic | Lab-on-Chip real-time RT-PCR System-on-Glass virus detection |
| url | https://www.mdpi.com/2079-6374/13/5/544 |
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