Traditional and molecular diagnosis of Haemonchus contortus in sheep in Babylon province, Iraq
<em>Haemonchus contortus</em> one of gastrointestinal helminthes characterized by miner intraspecific variation and a major variation among species which exploited to determine species simultaneously depend on PCR techniques, by amplification of DNA from parasites so goal of study was tr...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2022-04-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://vetmedmosul.com/article_172920_ae6ef3ff652bdd060ea278e2d9eecd54.pdf |
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| author | Balkes F. Hade Suha T. Al-Biatee Haider M. Al-Rubaie |
| author_facet | Balkes F. Hade Suha T. Al-Biatee Haider M. Al-Rubaie |
| author_sort | Balkes F. Hade |
| collection | DOAJ |
| description | <em>Haemonchus contortus</em> one of gastrointestinal helminthes characterized by miner intraspecific variation and a major variation among species which exploited to determine species simultaneously depend on PCR techniques, by amplification of DNA from parasites so goal of study was traditional and molecular detection of this parasite in sheep. Experiment was conducted using adult worms collected from abomasum of sheep obtained from abattoir in Babylon province. All worms divided into two groups, first group for morphological study and second group stored in 70% ethanol for DNA extraction, ITS-2 spacer and 28S gene were amplified using PCR technique sequenced and analysis with a phylogenic tree.According to the available data this study recorded<em>Haemonchus contortus</em> in sheep depend on ITS-2 spacer and 28S gene sequences for the first time in Iraq with accession no. LC552170 and LC552171 using molecular data.The phylogeny analysis depended on ITS-2 spacer and 28S gene partial sequences were closely related and high identity 94% with Germany <em>H. similis</em> sequence MN708992.1 and 93% identity with New-Zealand and Austria <em>H. contortus</em> sequence KC998713.1 and KJ724288.1 respectively, with a low genetic variation among all comparison sequenced isolates. |
| first_indexed | 2024-12-13T07:25:19Z |
| format | Article |
| id | doaj.art-ef689a125c27443b943675fb1f6e542e |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-12-13T07:25:19Z |
| publishDate | 2022-04-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-ef689a125c27443b943675fb1f6e542e2022-12-21T23:55:20ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552022-04-0136247948110.33899/ijvs.2021.130533.1842172920Traditional and molecular diagnosis of Haemonchus contortus in sheep in Babylon province, IraqBalkes F. Hade0Suha T. Al-Biatee1Haider M. Al-Rubaie2Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Baghdad, IraqDepartment of Parasitology, College of Veterinary Medicine, University of Baghdad, Baghdad, IraqDepartment of Parasitology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq<em>Haemonchus contortus</em> one of gastrointestinal helminthes characterized by miner intraspecific variation and a major variation among species which exploited to determine species simultaneously depend on PCR techniques, by amplification of DNA from parasites so goal of study was traditional and molecular detection of this parasite in sheep. Experiment was conducted using adult worms collected from abomasum of sheep obtained from abattoir in Babylon province. All worms divided into two groups, first group for morphological study and second group stored in 70% ethanol for DNA extraction, ITS-2 spacer and 28S gene were amplified using PCR technique sequenced and analysis with a phylogenic tree.According to the available data this study recorded<em>Haemonchus contortus</em> in sheep depend on ITS-2 spacer and 28S gene sequences for the first time in Iraq with accession no. LC552170 and LC552171 using molecular data.The phylogeny analysis depended on ITS-2 spacer and 28S gene partial sequences were closely related and high identity 94% with Germany <em>H. similis</em> sequence MN708992.1 and 93% identity with New-Zealand and Austria <em>H. contortus</em> sequence KC998713.1 and KJ724288.1 respectively, with a low genetic variation among all comparison sequenced isolates.https://vetmedmosul.com/article_172920_ae6ef3ff652bdd060ea278e2d9eecd54.pdfhaemonchus contortussheepits-2 spacer28s genes |
| spellingShingle | Balkes F. Hade Suha T. Al-Biatee Haider M. Al-Rubaie Traditional and molecular diagnosis of Haemonchus contortus in sheep in Babylon province, Iraq Iraqi Journal of Veterinary Sciences haemonchus contortus sheep its-2 spacer 28s genes |
| title | Traditional and molecular diagnosis of Haemonchus contortus in sheep in Babylon province, Iraq |
| title_full | Traditional and molecular diagnosis of Haemonchus contortus in sheep in Babylon province, Iraq |
| title_fullStr | Traditional and molecular diagnosis of Haemonchus contortus in sheep in Babylon province, Iraq |
| title_full_unstemmed | Traditional and molecular diagnosis of Haemonchus contortus in sheep in Babylon province, Iraq |
| title_short | Traditional and molecular diagnosis of Haemonchus contortus in sheep in Babylon province, Iraq |
| title_sort | traditional and molecular diagnosis of haemonchus contortus in sheep in babylon province iraq |
| topic | haemonchus contortus sheep its-2 spacer 28s genes |
| url | https://vetmedmosul.com/article_172920_ae6ef3ff652bdd060ea278e2d9eecd54.pdf |
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