Quantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1.
Stable isotope labeling with amino acids in cell culture (SILAC) was used to quantitatively study the host cell gene expression profile, in order to achieve an unbiased overview of the protein expression changes in BHK-21 cells infected with FMDV serotype Asia 1. The SILAC-based approach identified...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0132384&type=printable |
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| author | Hui-Chen Guo Ye Jin Shi-Chong Han Shi-Qi Sun Yan-Quan Wei Xian-Ji Liu Xia Feng Ding Xiang Liu Xiang-Tao Liu |
| author_facet | Hui-Chen Guo Ye Jin Shi-Chong Han Shi-Qi Sun Yan-Quan Wei Xian-Ji Liu Xia Feng Ding Xiang Liu Xiang-Tao Liu |
| author_sort | Hui-Chen Guo |
| collection | DOAJ |
| description | Stable isotope labeling with amino acids in cell culture (SILAC) was used to quantitatively study the host cell gene expression profile, in order to achieve an unbiased overview of the protein expression changes in BHK-21 cells infected with FMDV serotype Asia 1. The SILAC-based approach identified overall 2,141 proteins, 153 of which showed significant alteration in the expression level 6 h post FMDV infection (57 up-regulated and 96 down-regulated). Among these proteins, six cellular proteins, including three down-regulated (VPS28, PKR, EVI5) and three up-regulated (LYPLA1, SEC62 and DARs), were selected according to the significance of the changes and/or the relationship with PKR. The expression level and pattern of the selected proteins were validated by immunoblotting and confocal microscopy. Furthermore, the functions of these cellular proteins were assessed by small interfering RNA-mediated depletion, and their functional importance in the replication of FMDV was demonstrated by western blot, reverse transcript PCR (RT-PCR) and 50% Tissue Culture Infective Dose (TCID50). The results suggest that FMDV infection may have effects on the expression of specific cellular proteins to create more favorable conditions for FMDV infection. This study provides novel data that can be utilized to understand the interactions between FMDV and the host cell. |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
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| spelling | doaj.art-ef6a856264b14e789129d163d7dde7aa2025-02-23T05:31:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01107e013238410.1371/journal.pone.0132384Quantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1.Hui-Chen GuoYe JinShi-Chong HanShi-Qi SunYan-Quan WeiXian-Ji LiuXia FengDing Xiang LiuXiang-Tao LiuStable isotope labeling with amino acids in cell culture (SILAC) was used to quantitatively study the host cell gene expression profile, in order to achieve an unbiased overview of the protein expression changes in BHK-21 cells infected with FMDV serotype Asia 1. The SILAC-based approach identified overall 2,141 proteins, 153 of which showed significant alteration in the expression level 6 h post FMDV infection (57 up-regulated and 96 down-regulated). Among these proteins, six cellular proteins, including three down-regulated (VPS28, PKR, EVI5) and three up-regulated (LYPLA1, SEC62 and DARs), were selected according to the significance of the changes and/or the relationship with PKR. The expression level and pattern of the selected proteins were validated by immunoblotting and confocal microscopy. Furthermore, the functions of these cellular proteins were assessed by small interfering RNA-mediated depletion, and their functional importance in the replication of FMDV was demonstrated by western blot, reverse transcript PCR (RT-PCR) and 50% Tissue Culture Infective Dose (TCID50). The results suggest that FMDV infection may have effects on the expression of specific cellular proteins to create more favorable conditions for FMDV infection. This study provides novel data that can be utilized to understand the interactions between FMDV and the host cell.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0132384&type=printable |
| spellingShingle | Hui-Chen Guo Ye Jin Shi-Chong Han Shi-Qi Sun Yan-Quan Wei Xian-Ji Liu Xia Feng Ding Xiang Liu Xiang-Tao Liu Quantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1. PLoS ONE |
| title | Quantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1. |
| title_full | Quantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1. |
| title_fullStr | Quantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1. |
| title_full_unstemmed | Quantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1. |
| title_short | Quantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1. |
| title_sort | quantitative proteomic analysis of bhk 21 cells infected with foot and mouth disease virus serotype asia 1 |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0132384&type=printable |
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