Application of a novel fluorogenic polyurethane analogue probe in polyester‐degrading microorganisms screening by microfluidic droplet
Abstract Application of polyester‐degrading microorganisms or enzymes should be considered as an eco‐friendly alternative to chemical recycling due to the huge plastic waste disposal nowadays. However, current impranil DLN‐based screening of polyester‐degrading microorganisms is time‐consuming, labo...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-02-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.14121 |
| _version_ | 1797944873303670784 |
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| author | Anming Xu Jiawei Liu Shixiang Cao Bin Xu Chengzhi Guo Ziyi Yu Xiaoqiang Chen Jie Zhou Weiliang Dong Min Jiang |
| author_facet | Anming Xu Jiawei Liu Shixiang Cao Bin Xu Chengzhi Guo Ziyi Yu Xiaoqiang Chen Jie Zhou Weiliang Dong Min Jiang |
| author_sort | Anming Xu |
| collection | DOAJ |
| description | Abstract Application of polyester‐degrading microorganisms or enzymes should be considered as an eco‐friendly alternative to chemical recycling due to the huge plastic waste disposal nowadays. However, current impranil DLN‐based screening of polyester‐degrading microorganisms is time‐consuming, labour‐intensive and unable to distinguish polyesterases from other protease‐ or amidase‐like enzymes. Herein, we present an approach that combined a novel synthetic fluorescent polyurethane analogue probe (FPAP), along with the droplet‐based microfluidics to screen polyurethane‐degrading microorganisms through fluorescence‐activated droplet sorting (FADS) pipeline. The fluorescent probe FPAP exhibited a fluorescence enhancement effect once hydrolysed by polyesterases, along with a strong specificity in discriminating polyesterases from other non‐active enzymes. Application of FPAP in a microfluidic droplet system demonstrated that this probe exhibited high sensitivity and efficiency in selecting positive droplets containing leaf‐branch compost cutinase (LCC) enzymes. This novel fluorogenic probe, FPAP, combined with the droplet microfluidic system has the potential to be used in the exploitation of novel PUR‐biocatalysts for biotechnological and environmental applications. |
| first_indexed | 2024-04-10T20:46:27Z |
| format | Article |
| id | doaj.art-ef6d37433489410184724d6634474e33 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-04-10T20:46:27Z |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-ef6d37433489410184724d6634474e332023-01-24T07:57:37ZengWileyMicrobial Biotechnology1751-79152023-02-0116247448010.1111/1751-7915.14121Application of a novel fluorogenic polyurethane analogue probe in polyester‐degrading microorganisms screening by microfluidic dropletAnming Xu0Jiawei Liu1Shixiang Cao2Bin Xu3Chengzhi Guo4Ziyi Yu5Xiaoqiang Chen6Jie Zhou7Weiliang Dong8Min Jiang9College of Biotechnology and Pharmaceutical Engineering Nanjing Tech University Nanjing ChinaCollege of Biotechnology and Pharmaceutical Engineering Nanjing Tech University Nanjing ChinaCollege of Biotechnology and Pharmaceutical Engineering Nanjing Tech University Nanjing ChinaCollege of Biotechnology and Pharmaceutical Engineering Nanjing Tech University Nanjing ChinaState Key Laboratory of Materials‐Oriented Chemical Engineering Nanjing Tech University Nanjing ChinaState Key Laboratory of Materials‐Oriented Chemical Engineering Nanjing Tech University Nanjing ChinaState Key Laboratory of Materials‐Oriented Chemical Engineering Nanjing Tech University Nanjing ChinaCollege of Biotechnology and Pharmaceutical Engineering Nanjing Tech University Nanjing ChinaCollege of Biotechnology and Pharmaceutical Engineering Nanjing Tech University Nanjing ChinaCollege of Biotechnology and Pharmaceutical Engineering Nanjing Tech University Nanjing ChinaAbstract Application of polyester‐degrading microorganisms or enzymes should be considered as an eco‐friendly alternative to chemical recycling due to the huge plastic waste disposal nowadays. However, current impranil DLN‐based screening of polyester‐degrading microorganisms is time‐consuming, labour‐intensive and unable to distinguish polyesterases from other protease‐ or amidase‐like enzymes. Herein, we present an approach that combined a novel synthetic fluorescent polyurethane analogue probe (FPAP), along with the droplet‐based microfluidics to screen polyurethane‐degrading microorganisms through fluorescence‐activated droplet sorting (FADS) pipeline. The fluorescent probe FPAP exhibited a fluorescence enhancement effect once hydrolysed by polyesterases, along with a strong specificity in discriminating polyesterases from other non‐active enzymes. Application of FPAP in a microfluidic droplet system demonstrated that this probe exhibited high sensitivity and efficiency in selecting positive droplets containing leaf‐branch compost cutinase (LCC) enzymes. This novel fluorogenic probe, FPAP, combined with the droplet microfluidic system has the potential to be used in the exploitation of novel PUR‐biocatalysts for biotechnological and environmental applications.https://doi.org/10.1111/1751-7915.14121 |
| spellingShingle | Anming Xu Jiawei Liu Shixiang Cao Bin Xu Chengzhi Guo Ziyi Yu Xiaoqiang Chen Jie Zhou Weiliang Dong Min Jiang Application of a novel fluorogenic polyurethane analogue probe in polyester‐degrading microorganisms screening by microfluidic droplet Microbial Biotechnology |
| title | Application of a novel fluorogenic polyurethane analogue probe in polyester‐degrading microorganisms screening by microfluidic droplet |
| title_full | Application of a novel fluorogenic polyurethane analogue probe in polyester‐degrading microorganisms screening by microfluidic droplet |
| title_fullStr | Application of a novel fluorogenic polyurethane analogue probe in polyester‐degrading microorganisms screening by microfluidic droplet |
| title_full_unstemmed | Application of a novel fluorogenic polyurethane analogue probe in polyester‐degrading microorganisms screening by microfluidic droplet |
| title_short | Application of a novel fluorogenic polyurethane analogue probe in polyester‐degrading microorganisms screening by microfluidic droplet |
| title_sort | application of a novel fluorogenic polyurethane analogue probe in polyester degrading microorganisms screening by microfluidic droplet |
| url | https://doi.org/10.1111/1751-7915.14121 |
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