Structural characteristics of collagen from cuttlefish skin waste extracted at optimized conditions
Aquatic by-products during fish processing cause environmental pollution and increase disposal costs. Cuttlefish skin is one of the major by-products of cuttlefish (Sepia pharaonis) processing and is generally thrown as a by-product. It can cause severe environmental problems and odor. It has been d...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | International Journal of Food Properties |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10942912.2022.2127762 |
| _version_ | 1811230747194818560 |
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| author | Sulfath Hakkim Hazeena Ming-Kuei Shih Shu-Ling Hsieh Chang-Wei Hsieh Tsu Tung Liu Min-Hung Chen Yu-Wen Huang Chih-Yao Hou |
| author_facet | Sulfath Hakkim Hazeena Ming-Kuei Shih Shu-Ling Hsieh Chang-Wei Hsieh Tsu Tung Liu Min-Hung Chen Yu-Wen Huang Chih-Yao Hou |
| author_sort | Sulfath Hakkim Hazeena |
| collection | DOAJ |
| description | Aquatic by-products during fish processing cause environmental pollution and increase disposal costs. Cuttlefish skin is one of the major by-products of cuttlefish (Sepia pharaonis) processing and is generally thrown as a by-product. It can cause severe environmental problems and odor. It has been discovered that cuttlefish skin can be an excellent resource for producing attractive amounts of collagen. This study optimized collagen extraction conditions using response surface methodology (RSM). In addition, SDS-PAGE, specific charge, and scanning electron microscopy (SEM) characteristics of extracted collagen were evaluated. The results showed that the optimal extraction conditions for cuttlefish skin collagen are pH 1.5, 20 mg/L (solid-liquid ratio), 15 U/mg (Pepsin), and the average extraction rate can be obtained as 8.79%. The results of LC/MS/MS analysis of the collagen samples extracted in this study showed that the main m/z signal of proline produced by mass spectrometry was between 2000–4000. Collagen extracted from the cuttlefish skin is type I collagen, which consists of 2 α chains and 1 β chain (α2, α1, β). SEM analysis of collagen confirmed the presence of collagen fibrils in the cuttlefish skin similar to previous reports. Using the response surface methodology, optimal collagen extraction conditions pH 1.5, 20 mg/L (solid-liquid ratio), and 15 U/mg (Pepsin) can be obtained to recycle and utilize by-products. |
| first_indexed | 2024-04-12T10:34:14Z |
| format | Article |
| id | doaj.art-ef6f3d1da6014bdea095812c7c7b17c3 |
| institution | Directory Open Access Journal |
| issn | 1094-2912 1532-2386 |
| language | English |
| last_indexed | 2024-04-12T10:34:14Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Food Properties |
| spelling | doaj.art-ef6f3d1da6014bdea095812c7c7b17c32022-12-22T03:36:47ZengTaylor & Francis GroupInternational Journal of Food Properties1094-29121532-23862022-12-012512211222210.1080/10942912.2022.2127762Structural characteristics of collagen from cuttlefish skin waste extracted at optimized conditionsSulfath Hakkim Hazeena0Ming-Kuei Shih1Shu-Ling Hsieh2Chang-Wei Hsieh3Tsu Tung Liu4Min-Hung Chen5Yu-Wen Huang6Chih-Yao Hou7Department of Seafood Science, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung, TaiwanGraduate Institute of Food Culture and Innovation, National Kaohsiung University of Hospitality and Tourism, Kaohsiung, TaiwanDepartment of Seafood Science, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung, TaiwanDepartment of Food Science and Biotechnology, National Chung Hsing University, Taichung, TaiwanDepartment of Seafood Science, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung, TaiwanYuan Marketing & Processing Division, Agriculture & Food Agency Council of Agriculture Executive, Nantou, TaiwanDepartment of Seafood Science, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung, TaiwanDepartment of Seafood Science, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung, TaiwanAquatic by-products during fish processing cause environmental pollution and increase disposal costs. Cuttlefish skin is one of the major by-products of cuttlefish (Sepia pharaonis) processing and is generally thrown as a by-product. It can cause severe environmental problems and odor. It has been discovered that cuttlefish skin can be an excellent resource for producing attractive amounts of collagen. This study optimized collagen extraction conditions using response surface methodology (RSM). In addition, SDS-PAGE, specific charge, and scanning electron microscopy (SEM) characteristics of extracted collagen were evaluated. The results showed that the optimal extraction conditions for cuttlefish skin collagen are pH 1.5, 20 mg/L (solid-liquid ratio), 15 U/mg (Pepsin), and the average extraction rate can be obtained as 8.79%. The results of LC/MS/MS analysis of the collagen samples extracted in this study showed that the main m/z signal of proline produced by mass spectrometry was between 2000–4000. Collagen extracted from the cuttlefish skin is type I collagen, which consists of 2 α chains and 1 β chain (α2, α1, β). SEM analysis of collagen confirmed the presence of collagen fibrils in the cuttlefish skin similar to previous reports. Using the response surface methodology, optimal collagen extraction conditions pH 1.5, 20 mg/L (solid-liquid ratio), and 15 U/mg (Pepsin) can be obtained to recycle and utilize by-products.https://www.tandfonline.com/doi/10.1080/10942912.2022.2127762Sepia pharaonisAquatic by-productscollagenResponse surface methodologyScanning electron microscope |
| spellingShingle | Sulfath Hakkim Hazeena Ming-Kuei Shih Shu-Ling Hsieh Chang-Wei Hsieh Tsu Tung Liu Min-Hung Chen Yu-Wen Huang Chih-Yao Hou Structural characteristics of collagen from cuttlefish skin waste extracted at optimized conditions International Journal of Food Properties Sepia pharaonis Aquatic by-products collagen Response surface methodology Scanning electron microscope |
| title | Structural characteristics of collagen from cuttlefish skin waste extracted at optimized conditions |
| title_full | Structural characteristics of collagen from cuttlefish skin waste extracted at optimized conditions |
| title_fullStr | Structural characteristics of collagen from cuttlefish skin waste extracted at optimized conditions |
| title_full_unstemmed | Structural characteristics of collagen from cuttlefish skin waste extracted at optimized conditions |
| title_short | Structural characteristics of collagen from cuttlefish skin waste extracted at optimized conditions |
| title_sort | structural characteristics of collagen from cuttlefish skin waste extracted at optimized conditions |
| topic | Sepia pharaonis Aquatic by-products collagen Response surface methodology Scanning electron microscope |
| url | https://www.tandfonline.com/doi/10.1080/10942912.2022.2127762 |
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