Oxidative stress-apoptosis mediated STZ-induced diabetic cataract and the interventions of puerarin
AIM: To explore the involvement of oxidative stress and apoptosis in the pathogenesis of diabetic cataract induced by Streptozotocin(STZ)and the interventions of puerarin in order to supply references for clinical treatment.<p>METHODS: Male SD rats were divided into four groups randomly, contr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Press of International Journal of Ophthalmology (IJO PRESS)
2014-10-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://www.ies.net.cn/cn_publish/2014/10/201410008.pdf |
| Summary: | AIM: To explore the involvement of oxidative stress and apoptosis in the pathogenesis of diabetic cataract induced by Streptozotocin(STZ)and the interventions of puerarin in order to supply references for clinical treatment.<p>METHODS: Male SD rats were divided into four groups randomly, control group, diabetic group, apocynin group and puerarin group. The diabetic group were replicated by single injection of STZ(65mg/kg, ip). The expression of p22, p47, p67, Bax/Bcl2, Caspase 3 and P53 proteins were detected by Western Blotting. <p>RESULTS: The diabetic rats were replicated successfully and the expression of Bcl2 was downregulated while the expression of p22, p47, p67, Bax, Caspase 3 and P53 were upregulated in diabetic group with a significant statistical differences when compared with control group(<i>P</i><0.05). Apocynin and prerarin can reverse the abnormal expression of the aforementioned proteins dramatically(<i>P</i><0.05).<p>CONCLUSION: NADPH oxidase mediated oxidative stress and P53, Bax/Bcl2 mediated apoptosis are involved in the pathogenesis of diabetic cataract and puerarin can alleviate cataract greatly by inhibiting the aforementioned signal pathway. |
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| ISSN: | 1672-5123 1672-5123 |