First report of Bartonella henselae infection in a cat in North Macedonia
The genus Bartonella are fastidious, Gram-negative intracellular vector-borne bacteria with zoonotic potential. Cats have a crucial epidemiological role in the transmission of Bartonella henselae, the primary causal agent of cat-scratch disease in humans. Here we describe the first report of B. hens...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Ss.Cyril and Methodius University, Skopje
2023-10-01
|
| Series: | Macedonian Veterinary Review |
| Subjects: | |
| Online Access: | https://macvetrev.mk/LoadArticleContent?DOI=10.2478_macvetrev_2023_0028 |
| _version_ | 1827791970481733632 |
|---|---|
| author | Ivana Shikoska Igor Djadjovski Kiril Krstevski Zagorka Popova Hristovska Aleksandar Janevski Ljubica Rashikj Aleksandar Cvetkovikj Iskra Cvetkovikj |
| author_facet | Ivana Shikoska Igor Djadjovski Kiril Krstevski Zagorka Popova Hristovska Aleksandar Janevski Ljubica Rashikj Aleksandar Cvetkovikj Iskra Cvetkovikj |
| author_sort | Ivana Shikoska |
| collection | DOAJ |
| description | The genus Bartonella are fastidious, Gram-negative intracellular vector-borne bacteria with zoonotic potential. Cats have a crucial epidemiological role in the transmission of Bartonella henselae, the primary causal agent of cat-scratch disease in humans. Here we describe the first report of B. henselae infection in a cat in North Macedonia after a suspected Bartonella infection in a 7-year-old boy. A combination of cultural and molecular methods for detecting B. henselae in the asymptomatic cat was used. A blood sample was cultured on a blood agar plate, where colonies became visible after nine days of incubation. The DNA of a single colony was extracted, and the isolate was confirmed as B. henselae by sequencing the bacterial 16S ribosomal RNA gene and performing a Bartonella - specific PCR. The phylogenetic analysis demonstrated 100% similarity with B. henselae sequences, clustering on a single branch and establishing a distinct group. This finding indicates that B. henselae infection in cats circulates in North Macedonia and may affect feline and human health. |
| first_indexed | 2024-03-11T17:55:36Z |
| format | Article |
| id | doaj.art-ef71d882132246ef9fb27878d5a77c0c |
| institution | Directory Open Access Journal |
| issn | 1409-7621 1857-7415 |
| language | English |
| last_indexed | 2024-03-11T17:55:36Z |
| publishDate | 2023-10-01 |
| publisher | Ss.Cyril and Methodius University, Skopje |
| record_format | Article |
| series | Macedonian Veterinary Review |
| spelling | doaj.art-ef71d882132246ef9fb27878d5a77c0c2023-10-17T13:40:21ZengSs.Cyril and Methodius University, SkopjeMacedonian Veterinary Review1409-76211857-74152023-10-0146220721310.2478/macvetrev-2023-0028First report of Bartonella henselae infection in a cat in North MacedoniaIvana Shikoska0https://orcid.org/0000-0002-7605-3825Igor Djadjovski1https://orcid.org/0000-0002-4820-3590Kiril Krstevski2https://orcid.org/0000-0001-5453-3329Zagorka Popova Hristovska3https://orcid.org/0009-0007-0521-7603Aleksandar Janevski4https://orcid.org/0009-0000-5276-4991Ljubica Rashikj5https://orcid.org/0000-0002-9617-5811Aleksandar Cvetkovikj6https://orcid.org/0000-0002-6582-7020Iskra Cvetkovikj7https://orcid.org/0000-0001-6697-8034Veterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, North MacedoniaVeterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, North MacedoniaVeterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, North MacedoniaVeterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, North MacedoniaVeterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, North MacedoniaVeterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, North MacedoniaVeterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, North MacedoniaVeterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, North MacedoniaThe genus Bartonella are fastidious, Gram-negative intracellular vector-borne bacteria with zoonotic potential. Cats have a crucial epidemiological role in the transmission of Bartonella henselae, the primary causal agent of cat-scratch disease in humans. Here we describe the first report of B. henselae infection in a cat in North Macedonia after a suspected Bartonella infection in a 7-year-old boy. A combination of cultural and molecular methods for detecting B. henselae in the asymptomatic cat was used. A blood sample was cultured on a blood agar plate, where colonies became visible after nine days of incubation. The DNA of a single colony was extracted, and the isolate was confirmed as B. henselae by sequencing the bacterial 16S ribosomal RNA gene and performing a Bartonella - specific PCR. The phylogenetic analysis demonstrated 100% similarity with B. henselae sequences, clustering on a single branch and establishing a distinct group. This finding indicates that B. henselae infection in cats circulates in North Macedonia and may affect feline and human health.https://macvetrev.mk/LoadArticleContent?DOI=10.2478_macvetrev_2023_0028bartonella henselaezoonosispublic healthdetection |
| spellingShingle | Ivana Shikoska Igor Djadjovski Kiril Krstevski Zagorka Popova Hristovska Aleksandar Janevski Ljubica Rashikj Aleksandar Cvetkovikj Iskra Cvetkovikj First report of Bartonella henselae infection in a cat in North Macedonia Macedonian Veterinary Review bartonella henselae zoonosis public health detection |
| title | First report of Bartonella henselae infection in a cat in North Macedonia |
| title_full | First report of Bartonella henselae infection in a cat in North Macedonia |
| title_fullStr | First report of Bartonella henselae infection in a cat in North Macedonia |
| title_full_unstemmed | First report of Bartonella henselae infection in a cat in North Macedonia |
| title_short | First report of Bartonella henselae infection in a cat in North Macedonia |
| title_sort | first report of bartonella henselae infection in a cat in north macedonia |
| topic | bartonella henselae zoonosis public health detection |
| url | https://macvetrev.mk/LoadArticleContent?DOI=10.2478_macvetrev_2023_0028 |
| work_keys_str_mv | AT ivanashikoska firstreportofbartonellahenselaeinfectioninacatinnorthmacedonia AT igordjadjovski firstreportofbartonellahenselaeinfectioninacatinnorthmacedonia AT kirilkrstevski firstreportofbartonellahenselaeinfectioninacatinnorthmacedonia AT zagorkapopovahristovska firstreportofbartonellahenselaeinfectioninacatinnorthmacedonia AT aleksandarjanevski firstreportofbartonellahenselaeinfectioninacatinnorthmacedonia AT ljubicarashikj firstreportofbartonellahenselaeinfectioninacatinnorthmacedonia AT aleksandarcvetkovikj firstreportofbartonellahenselaeinfectioninacatinnorthmacedonia AT iskracvetkovikj firstreportofbartonellahenselaeinfectioninacatinnorthmacedonia |