Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells
Cells of Saccharum officinarum submitted to hydrolyzated chitin for 1 to 8h produced phenolic compounds. These alterations were observed through cytochemical methods using Toluidine Blue and Phloroglucinol/HCl. After 4 h, besides cell wall change, there was a change in nuclear pattern of chitin trea...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2010-04-01
|
| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200002 |
| _version_ | 1819046477184368640 |
|---|---|
| author | Maria Izabel Gallão Ângelo Luiz Cortelazzo Manuel Pedro Salema Fevereiro Edy Sousa de Brito |
| author_facet | Maria Izabel Gallão Ângelo Luiz Cortelazzo Manuel Pedro Salema Fevereiro Edy Sousa de Brito |
| author_sort | Maria Izabel Gallão |
| collection | DOAJ |
| description | Cells of Saccharum officinarum submitted to hydrolyzated chitin for 1 to 8h produced phenolic compounds. These alterations were observed through cytochemical methods using Toluidine Blue and Phloroglucinol/HCl. After 4 h, besides cell wall change, there was a change in nuclear pattern of chitin treated cells. There was a 96% increase in nuclear area in 6 h chitin treated material, as observed by Feulgen reaction. The treated cells showed chromatin compacted regions and a degeneration process of nucleoli. In the outer areas of cell wall, there was a polysaccharide desagregation, confirming results obtained for different plants with the use of other elicitors. Peroxidase activity was maximal after 4 h and decreased progressively. PAL activity started to increase at 4 h of incubation. These results showed that chitin hydrolyzate stimulated a defense response in sugarcane cells.<br>Células de Saccharum officinarum quando submetidas a quitina hidrolisada por 1 a 8h produziram material fenólico. Essas alterações foram observadas por meio de métodos citoquímicos como o Azul de Toluidina e Floroglucinol/HCl. Após 4 h, além das mudanças nas paredes celulares houve uma mudança no padrão nuclear das células tratadas com quitina. Por observação da reação de Feulgen, houve um aumento de 96% na área nuclear no material em 6h. Para as células tratadas foram observadas regiões de cromatina compactada e um processo de degeneração do nucléolo. Nas áreas externas da parede celular existia uma desagregação dos polisacarídios confirmando os resultados obtidos para diferentes plantas com o uso de outros elicitores. A atividade da peroxidase foi maxima após 4 h e então decresceu progressivamente. A atividade da PAL aumentou a partir de 4 h de incubação. Estes resultados mostram que o hidrolisado de quitina estimula as respostas de defesa em células de cana. |
| first_indexed | 2024-12-21T10:45:05Z |
| format | Article |
| id | doaj.art-ef73235f307240f6b5d9b7294f0638e2 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-21T10:45:05Z |
| publishDate | 2010-04-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-ef73235f307240f6b5d9b7294f0638e22022-12-21T19:06:50ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242010-04-0153225326010.1590/S1516-89132010000200002Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cellsMaria Izabel GallãoÂngelo Luiz CortelazzoManuel Pedro Salema FevereiroEdy Sousa de BritoCells of Saccharum officinarum submitted to hydrolyzated chitin for 1 to 8h produced phenolic compounds. These alterations were observed through cytochemical methods using Toluidine Blue and Phloroglucinol/HCl. After 4 h, besides cell wall change, there was a change in nuclear pattern of chitin treated cells. There was a 96% increase in nuclear area in 6 h chitin treated material, as observed by Feulgen reaction. The treated cells showed chromatin compacted regions and a degeneration process of nucleoli. In the outer areas of cell wall, there was a polysaccharide desagregation, confirming results obtained for different plants with the use of other elicitors. Peroxidase activity was maximal after 4 h and decreased progressively. PAL activity started to increase at 4 h of incubation. These results showed that chitin hydrolyzate stimulated a defense response in sugarcane cells.<br>Células de Saccharum officinarum quando submetidas a quitina hidrolisada por 1 a 8h produziram material fenólico. Essas alterações foram observadas por meio de métodos citoquímicos como o Azul de Toluidina e Floroglucinol/HCl. Após 4 h, além das mudanças nas paredes celulares houve uma mudança no padrão nuclear das células tratadas com quitina. Por observação da reação de Feulgen, houve um aumento de 96% na área nuclear no material em 6h. Para as células tratadas foram observadas regiões de cromatina compactada e um processo de degeneração do nucléolo. Nas áreas externas da parede celular existia uma desagregação dos polisacarídios confirmando os resultados obtidos para diferentes plantas com o uso de outros elicitores. A atividade da peroxidase foi maxima após 4 h e então decresceu progressivamente. A atividade da PAL aumentou a partir de 4 h de incubação. Estes resultados mostram que o hidrolisado de quitina estimula as respostas de defesa em células de cana.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200002ChitinelicitationperoxidasePALsugarcanetissue culture |
| spellingShingle | Maria Izabel Gallão Ângelo Luiz Cortelazzo Manuel Pedro Salema Fevereiro Edy Sousa de Brito Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells Brazilian Archives of Biology and Technology Chitin elicitation peroxidase PAL sugarcane tissue culture |
| title | Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells |
| title_full | Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells |
| title_fullStr | Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells |
| title_full_unstemmed | Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells |
| title_short | Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells |
| title_sort | biochemical and morphological responses to abiotc elicitor chitin in suspension cultured sugarcane cells |
| topic | Chitin elicitation peroxidase PAL sugarcane tissue culture |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200002 |
| work_keys_str_mv | AT mariaizabelgallao biochemicalandmorphologicalresponsestoabiotcelicitorchitininsuspensionculturedsugarcanecells AT angeloluizcortelazzo biochemicalandmorphologicalresponsestoabiotcelicitorchitininsuspensionculturedsugarcanecells AT manuelpedrosalemafevereiro biochemicalandmorphologicalresponsestoabiotcelicitorchitininsuspensionculturedsugarcanecells AT edysousadebrito biochemicalandmorphologicalresponsestoabiotcelicitorchitininsuspensionculturedsugarcanecells |