Physical properties, biological applications and biocompatibility studies on biosynthesized single phase cobalt oxide (Co3O4) nanoparticles via Sageretia thea (Osbeck.)
Cobalt oxide nanoparticles were successfully biosynthesized by complete green process using aqueous leaf extracts of Sageretia thea as chelating agent. Diverse techniques were applied for characterization. Antibacterial (with and without UV illumination), antileishmanial, antioxidant and enzyme inhi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | Arabian Journal of Chemistry |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187853521730134X |
| _version_ | 1818555713965785088 |
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| author | Ali Talha Khalil Muhammad Ovais Ikram Ullah Muhammad Ali Zabta Khan Shinwari Malik Maaza |
| author_facet | Ali Talha Khalil Muhammad Ovais Ikram Ullah Muhammad Ali Zabta Khan Shinwari Malik Maaza |
| author_sort | Ali Talha Khalil |
| collection | DOAJ |
| description | Cobalt oxide nanoparticles were successfully biosynthesized by complete green process using aqueous leaf extracts of Sageretia thea as chelating agent. Diverse techniques were applied for characterization. Antibacterial (with and without UV illumination), antileishmanial, antioxidant and enzyme inhibition applications were assessed, while freshly isolated macrophages and red blood cells were used for biocompatibility studies. Good antibacterial nature and enhancement of bactericidal nature upon UV modulation is reported. Staphylococcus aureus and Escherichia coli are indicated as most susceptible bacterial strains. Significant cytotoxic potential is revealed with IC50 calculated as 12.82 µg/ml and 3.16 µg/ml against the axenic leishmanial promastigote and amastigote cultures respectively. Biogenic cobalt oxide nanoparticles indicated DPPH free radical scavenging potential, while moderate antioxidant capacity and reducing power was demonstrated. Bioinspired cobalt oxide also demonstrated alpha amylase and protein kinase inhibition at higher concentrations. Biogenic cobalt oxide was found as more cytotoxic to macrophages (IC50 = 58.55 µg/ml) then to RBC’s (IC50 >200 µg/ml). Our results indicate green synthesis as an alternative, effective and eco-friendly method for the biosynthesis of cobalt oxide nanoparticles with numerous biological applications. Keywords: Biosynthesis, Cobalt oxide, Nanoparticles, Antimicrobial, Antileishmanial, Antioxidant |
| first_indexed | 2024-12-12T09:57:12Z |
| format | Article |
| id | doaj.art-ef74f1e5abed4d7e987975d9f83def08 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-12T09:57:12Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-ef74f1e5abed4d7e987975d9f83def082022-12-22T00:28:06ZengElsevierArabian Journal of Chemistry1878-53522020-01-01131606619Physical properties, biological applications and biocompatibility studies on biosynthesized single phase cobalt oxide (Co3O4) nanoparticles via Sageretia thea (Osbeck.)Ali Talha Khalil0Muhammad Ovais1Ikram Ullah2Muhammad Ali3Zabta Khan Shinwari4Malik Maaza5Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan; UNESCO-UNISA Africa Chair in Nanosciences and Nanotechnology, College of Graduate Studies, University of South Africa, Pretoria, South Africa; Nanosciences African Network (NANOAFNET), Materials Research Department, iThemba Labs, Westren Cape, South Africa; Corresponding author at: Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan.Department of Biotechnology, Quaid-i-Azam University, Islamabad, PakistanDepartment of Biotechnology, Quaid-i-Azam University, Islamabad, PakistanDepartment of Biotechnology, Quaid-i-Azam University, Islamabad, PakistanDepartment of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan; Pakistan Academy of Sciences, PakistanUNESCO-UNISA Africa Chair in Nanosciences and Nanotechnology, College of Graduate Studies, University of South Africa, Pretoria, South Africa; Nanosciences African Network (NANOAFNET), Materials Research Department, iThemba Labs, Westren Cape, South AfricaCobalt oxide nanoparticles were successfully biosynthesized by complete green process using aqueous leaf extracts of Sageretia thea as chelating agent. Diverse techniques were applied for characterization. Antibacterial (with and without UV illumination), antileishmanial, antioxidant and enzyme inhibition applications were assessed, while freshly isolated macrophages and red blood cells were used for biocompatibility studies. Good antibacterial nature and enhancement of bactericidal nature upon UV modulation is reported. Staphylococcus aureus and Escherichia coli are indicated as most susceptible bacterial strains. Significant cytotoxic potential is revealed with IC50 calculated as 12.82 µg/ml and 3.16 µg/ml against the axenic leishmanial promastigote and amastigote cultures respectively. Biogenic cobalt oxide nanoparticles indicated DPPH free radical scavenging potential, while moderate antioxidant capacity and reducing power was demonstrated. Bioinspired cobalt oxide also demonstrated alpha amylase and protein kinase inhibition at higher concentrations. Biogenic cobalt oxide was found as more cytotoxic to macrophages (IC50 = 58.55 µg/ml) then to RBC’s (IC50 >200 µg/ml). Our results indicate green synthesis as an alternative, effective and eco-friendly method for the biosynthesis of cobalt oxide nanoparticles with numerous biological applications. Keywords: Biosynthesis, Cobalt oxide, Nanoparticles, Antimicrobial, Antileishmanial, Antioxidanthttp://www.sciencedirect.com/science/article/pii/S187853521730134X |
| spellingShingle | Ali Talha Khalil Muhammad Ovais Ikram Ullah Muhammad Ali Zabta Khan Shinwari Malik Maaza Physical properties, biological applications and biocompatibility studies on biosynthesized single phase cobalt oxide (Co3O4) nanoparticles via Sageretia thea (Osbeck.) Arabian Journal of Chemistry |
| title | Physical properties, biological applications and biocompatibility studies on biosynthesized single phase cobalt oxide (Co3O4) nanoparticles via Sageretia thea (Osbeck.) |
| title_full | Physical properties, biological applications and biocompatibility studies on biosynthesized single phase cobalt oxide (Co3O4) nanoparticles via Sageretia thea (Osbeck.) |
| title_fullStr | Physical properties, biological applications and biocompatibility studies on biosynthesized single phase cobalt oxide (Co3O4) nanoparticles via Sageretia thea (Osbeck.) |
| title_full_unstemmed | Physical properties, biological applications and biocompatibility studies on biosynthesized single phase cobalt oxide (Co3O4) nanoparticles via Sageretia thea (Osbeck.) |
| title_short | Physical properties, biological applications and biocompatibility studies on biosynthesized single phase cobalt oxide (Co3O4) nanoparticles via Sageretia thea (Osbeck.) |
| title_sort | physical properties biological applications and biocompatibility studies on biosynthesized single phase cobalt oxide co3o4 nanoparticles via sageretia thea osbeck |
| url | http://www.sciencedirect.com/science/article/pii/S187853521730134X |
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